Igsf8 (BC048387) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	PGRL, EWI-2, KCT-4
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC048387 GCTGGGCTCTGCGCACCCACGCTTCACTGCTCCGCTTTCTGCCCCCCACCTGGGCATGCGCCCCCCGCGG CCCTGGCCCCCCAGAGCCCACGCCGCCCCCAGGAGTCTCCTCAGAGCCGCCTACGCAGAATGGGCGTCCC TAGCCCCACGCCGCTGAGTTCGCTCCTGCTGCTGCTGCTAATACTTGGAACCAGGTGCTACGCCCGGCAG GTGCATGTCCCCAGGGGACCTCTTTACCGGGTGGCTGGCACCGCTGTCTCTATCTCCTGCAACGTGAGTG ACTATGAGGGCCCTGCCCAGCAAGACTTCGAGTGGTTCATGTACAGACCAGAGGCCCCAGCTACGTCCCT GGGCATTGTCAGCACCAAGGATAGCCAGTTCTCCTATGCTGTCTTTGGGCCTCGTGTGGCATCTGGTGAC CTGCAGGTGCAGCGCCTGAAGGGAGATTCGGTGGTGCTCAAGATTGCTCGCCTGCAGGCCCAGGACTCTG GCTTTTATGAGTGCTACACCCCCTCCACAGATACGCAGTACCTGGGCAACTACAGTGCCAAGGTGGAGCT GAGAGTTCTTCCAGATGAGCTGCAGGTATCTGCTGCCCCTCCAGGGCCCCGAGGGCGCCAGGCTGCCACC TCCCCCTCACGCCTCACAGTGCACGAGGGGCAGGAGCTTGCGCTGGGCTGCCTGGCTCAGACCAAAACAA AGAAACACACACACCTGTCGGTGTCCTTTGGGAGAGCCATTCCGGAGGCGCCAGTGGGGCGAGCCACTCT ACAGGAAGTAGTGGGACTGCACTCCGACATGGCGGTGGAGGCTGGAGCGCCCTACGCTGAGAGGCTAGCT TCTGGGGAGCTTCGGCTGAGCAAGGAAGGGACTGATCGGTACCGAATGGTGGTTGGGGGTGCCCAGGCTG GAGACTCGGGCACCTACCACTGTACGGCCGCTGAGTGGATTCAGGATCCTGACGGCTCCTGGGTCCAGGT CGCAGAGAAGAGGGCAGTCCTGGCTCATGTTGATGTGCAGACTCTGTCCAGCCAGCTGGCGGTGACCGTG GGACCTGGTGAACGTCGGATTGGCCCAGGCGAACCCTTAGAACTGCTGTGCAATGTGTCCGGTGCACTGC CCCCACCAGGCCGTCATGCTGCGTACTCTGTGGGCTGGGAGATGGCCCCTGCAGGGGCTCCTGGACCCGG CCGCCTGGTGGCCCAGCTGGACACGGAAGGTATAGGCAGCCTGGGCCCTGGCTATGAGGACCGGCACATT GCCATGGAGAAAGTAGCATCCAGAACCTACCGACTGCGGCTGGAGGCTGCCAGGCCCGCTGATGCAGGCA CCTACCGCTGCCTCGCCAAAGCCTATGTTCGAGGGTCTGGGACCCGGCTTCGTGAAGCGGCCAGTGCTCG TTCCCGGCCTCTCCCTGTGCATGTGAGAGAAGAAGGCGTGGTGCTAGAGGCTGTGGCGTGGCTAGCAGGG GGCACTGTGTACCGGGGAGAAACGGCCTCCCTGCTATGCAACATATCTGTGCGGGGCGGCCCCCCAGGGC TGCGACTAGCAGCCAGCTGGTGGGTGGAGAGGCCAGAGGAGGGCGAGCTGAGCACTGGCCCTGCTCAGCT TGTGGGTGGAGTGGGTCAGGACGGTGTGGCAGAGCTGGGAGTCCGGCCTGGAGGGGGTCCTGTCAGTGTG GAGCTGGTGGGACCCAGAAGTCATCGGCTAAGACTGCACGGCTTGGGGCCTGAGGATGAAGGCATATACC ACTGTGCCCCAAGTGCCTGGGTGCAGCATGCGGACTACAGCTGGTACCAGGCAGGCAGTGCGCGCTCCGG GCCTGTCACAGTCTACCCCTACACGCATGCTGTGGATACCCTATTTGTGCCCCTGTTGGTGGGTACAGGA GTCGCCCTAGTTACCGGCGCCAGCGTCCTGGCTACCATCACCTGCTGCTTTATGAAGAGGATGCGGAAGC GGTGATCTGTAGCACCCCAGTTCTTGCAGGTGTTGACTGTCTTCCAGCCAGTGCCACCCCTCCTCTGGTT GCCTGGATACCCTCTCCCTCTGTCCAGCCCACCTTTAATTTATTTGACCAGCCATCACCTAGAGTGGGAG ACATGGCTCCCCTGTTCCCTCCTTCCCTCAAGTTTCCTGGCTGCCGTTCTTAGTCCCACACTCATGTTGT AGGAAGTCCAGTTCCTGTCCTCAAACTAGTTTTTCTAAAATGTGAATAAACTGGTTTTGTAAAAAAAAAA AAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC048387
Insert Size	1836 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048387, AAH48387
RefSeq Size	2256 bp
RefSeq ORF	1836 bp
Locus ID	140559
Gene Summary	May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain.[UniProtKB/Swiss-Prot Function]

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