Fads2 (BC057189) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2900042M13Rik; Fadsd2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC057189 GTGTTGCGAGCCGCGGGCGCCCGGAGGCCGTAGCGCACGGGGCATCCGGGTAGACTGGCAGCATGGGGAA GGGAGGTAACCAGGGAGAGGGGAGCACCGAGCGCCAGGCTCCGATGCCCACCTTCCGTTGGGAGGAGATT CAGAAGCACAACCTGCGCACCGACCGGTGGCTCGTCATCGACCGCAAGGTCTACAACGTTACCAAATGGT CCCAGCGGCACCCGGGGGGCCACCGTGTCATCGGACACTATTCGGGAGAAGATGCTACGGATGCCTTCCG TGCCTTCCATCTGGACCTGGACTTCGTGGGCAAGTTCTTGAAGCCCCTGCTGATTGGTGAGCTGGCCCCA GAGGAGCCCAGCCTGGACCGTGGCAAAAGCTCTCAGATCACCGAGGACTTCAGGGCCCTGAAGAAGACTG CTGAGGACATGAACCTCTTCAAAACCAACCACCTGTTCTTCTTTCTCCTCCTGTCCCACATCATCGTCAT GGAAAGCCTTGCCTGGTTCATCCTCTCGTACTTCGGCACTGGCTGGATTCCTACCCTCGTCACAGCCTTT GTCCTCGCTACCTCTCAGGCCCAAGCTGGATGGCTGCAACATGACTATGGCCACCTTTCTGTCTATAAGA AATCCATATGGAACCACGTTGTCCACAAGTTTGTCATTGGCCACTTAAAGGGTGCCTCAGCCAACTGGTG GAACCACCGACATTTCCAACACCATGCCAAGCCCAACATCTTCCACAAGGACCCGGACATAAAGAGCCTG CATGTGTTTGTCCTTGGCGAGTGGCAGCCCCTTGAGTATGGCAAGAAGAAGCTGAAATACCTGCCCTACA ACCACCAGCATGAATACTTCTTCCTGATCGGACCGCCGCTGCTCATCCCTATGTACTTCCAGTACCAGAT CATCATGACAATGATCAGCCGCAGGGACTGGGTGGACTTGGCTTGGGCCATCAGCTACTATATGCGTTTC TTCTACACCTACATCCCTTTCTACGGCATCTTGGGAGCCCTGGTTTTCCTCAACTTTATCAGGTTCCTGG AGAGCCACTGGTTTGTGTGGGTCACACAGATGAACCACCTTGTCATGGAGATTGATCTTGATCACTACCG GGACTGGTTCAGCAGCCAGCTGGCAGCCACCTGCAATGTGGAGCAGTCCTTCTTCAATGACTGGTTCAGC GGGCACCTCAATTTCCAGATTGAGCACCACCTCTTCCCCACTATGCCACGTCACAACCTGCACAAGATTG CCCCACTGGTGAAGTCTCTCTGCGCCAAGCATGGCATTGAATACCAGGAGAAGCCGTTGCTGAGGGCCCT GATCGACATTGTGAGTTCACTGAAGAAGTCTGGGGAGCTGTGGCTGGATGCTTACCTCCATAAATGAAGC TGCCGTCCTCCGGGCACCCTCGGGAAAGGGGCACTGTTGGGTGACAGCCAGAGGGAGGGGAGGGCTTTTG TTCTGAAGGGTTCTCATGAGACTGAAGTGTCTGCTGCCTCACATTCCCCGGGCTTGCATCCTTTCTTGTT ATTCCTCTTCCCTCACCCTTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC057189
Insert Size	1335 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC057189, AAH57189
RefSeq Size	1608 bp
RefSeq ORF	1335 bp
Locus ID	56473
Gene Summary	Acts as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Involved in biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (PubMed:9867867). Subsequently, in the biosynthetic pathway of HUFA n-3 series, desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity). Desaturates palmitate to produce the mono-unsaturated fatty acid sapienate, the most abundant fatty acid in sebum (By similarity). Also desaturates (11E)-octadecenoate (trans-vaccenoate)(18:1n-9), a metabolite in the biohydrogenation pathway of LA (18:2n-6) (By similarity).[UniProtKB/Swiss-Prot Function]

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