Uhmk1 (BC058732) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4732477C12Rik; 4930500M09Rik; AA673513; AI449218; AU021979; C820018A03Rik; KIS; Kist; OTTMUSG00000021542; P-CIP2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC058732 GGTGTCATGGCGGCCTGAGGTCCGGGGAGTCGGTCGGAGCGCTGCAGGCCCCTCCCTGCGGAGCGGCGGG TCCGGGCGGGGATGTGACGGCGGGCGCCGCGGGCCTGCCTGGCGCGTCGTGTCGGCTCGCGTGTCCTCGG CCCAGCCCGCACCATGGCGGGGTCCGGCTGCGCGTGGGGCGCCGAGCCGCCGCGCTTCCTGGAGGCCTTC GGGCGGCTGTGGCAGGTCCAGAGCCGCCTGGGCAGCGGCTCGTCGGCCTCGGTGTACCGGGTGCGGTGCT GCGGTACCCCGGGCTCGCCCCCCGGCGCCCTCAAGCAGTTCCTGCCTCCGGGAACCACCGGGGCTGCGGC CTCGGCCGCGGAGTATGGTTTCCGCAAAGAGAGGGCGGCGCTGGAGCAGTTGCAGGGTCACAGGAACATC GTGACTTTATATGGAGTCTTTACCATACACTTCTCTCCCAATGTGCCATCACGCTGTCTGCTGCTTGAAC TCCTGGATGTCAGTGTTTCGGAATTGCTTTTATATTCCAGTCATCAGGGCTGCTCCATGTGGATGATACA GCACTGTGCCCGAGATGTTCTGGAGGCCCTCGCTTTTCTTCACCATGAGGGCTACGTCCATGCAGACCTC AAACCACGAAACATCCTGTGGAGTGCGGAGAATGAGTGTTTTAAGCTTATTGACTTTGGACTCAGCTTCA AAGAAGGCAATCAGGACGTAAAGTATATTCAGACAGACGGGTATCGCGCTCCTGAAGCAGAGCTGCAGAA CTGCTTGGCCCAGGCCGGCCTGCAGAGTGATACAGAATGTACCTCAGCTGTTGATCTGTGGAGCCTCGGA ATCATTTTACTGGAAATGTTCTCAGGAATGAAGCTGAAACATACAGTCAGATCTCAGGAATGGAAGGCAA ACAGTTCTGCTATTATTGATCATATATTTGCCAGTAAAGCAGTGGTGAATGCCGCAATTCCAGCCTATCA CCTCAGAGACCTTATCAAAAGCATGCTTCATGATGACCCGGGCAGAAGAATCCCTGCTGAGATGGCATTG TGCAGCCCATTCTTTAGCATTCCTTTTGCCCCTCATATTGAAGATCTGGTGATGCTTCCGACTCCAGTGC TCAGGCTCCTCAATGTGCTGGATGATGATTACCTTGAAAATGAAGATGAATATGAAGATGTTGTAGAAGA TGTGAAAGAGGAGTGTCAGAAATATGGACCAGTGGTTTCTCTGCTTGTTCCAAAGGAAAATCCTGGCAGA GGACAAGTCTTCGTTGAGTACGCGAACGCTGGTGATTCCAAAGCTGCTCAGAAGTTGCTGACTGGGAGGA TGTTTGACGGGAAGTTTGTTGTGGCTACATTCTACCCGCTGAGTGCCTACAAGAGGGGATATCTTTATCA AACCTTGCTTTAATCAGTAACCTAAGGACTACTTCCTGTTTCTCCTCTTCCATTTCTTGGATTATTATAT TCCACAGCTGAATGCAGGAGCACCCCTTTACCCATTTTAAAGGGTACCTTGTACATTTATTTAATCCTAC TAATGTGCAGCCATTGCCCAGACAGTGACTGCATTGCAAACATGGCACTGAGTAGGACCCGACCTCTCAG CTATACATTGAGGGGGTTAGAGCACCCATGTGGCCACCCTTTATTTGTGCAGTAGGGCAGGTGCTGCTCT TTACTATGTAACCTAAAAAGATGTCATTTCATGTGATGATGAAGCAAAGATGAGTTGTTTGTCATAGTAA ATACTATTAACAAATTTGTTAGGGTTGGTGACATCACAGAATATTTGTTTGTATTGTCAGGTGTATCTCC TTGTTATTCTAGCTGGCACTGGATGCTCTAATAATGTTAAGTCATTGTCAAGCAGCAGTTACCTACCGTG TTCTTAACAATGAGTTGTGAATTTTTCTAAAGGAGTACTGTAGGACTTAATATTCCTCCGAAGAAACTGC AGTGAGCCTATCAATGTTTTTAAAATTACTATTAAGGCTTTTAAAATAGAAAGCTGATGCTTGAAAAAAA AAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC058732
Insert Size	1260 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058732, AAH58732
RefSeq Size	2041 bp
RefSeq ORF	1260 bp
Locus ID	16589
Gene Summary	Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA (By similarity).[UniProtKB/Swiss-Prot Function]

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