Pja1 (BC037616) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	praja-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC037616 CCACGCGTCCGTGCAGCTCCGAGCGCGCGACTAGCCAGAAAGTTTCAGGCCATCCATGAGCCACCAGGAA AGGATTGCCAGCCAGAGGAGGACAACAGCCGAAGTCCCAATGCACAGATCAACTGCCAATCAAAGCAAGA GGAGCCGGTCACCATTTGCCAGCACACGTCGTCGCTGGGATGACAGCGAGAGCTCGGGAGCCAGCCTGGC TGTTGAGAGTGAGGATTATTCCAGGTATCCTCCAAGAGAGTACAGGGCCTCGGGGAGCCGAAGAGGATTG GCTTATGGACACATTGACACTGTAGTGGCTCGTGATAGTGAGGAGGAGGGGGCTGGGCCTGTTGACCGAC TGCCAGTGAGAGGGAAAGCTGGCAAGTTTAAGGATGATCCCGAGAAGGGGGCAAGGTCTTCCCGCTTTAC TAGTGTTAACCATGATGCGAAGGAAGAGTGTGGCAAGGTAGAATCACCCCCTGCAGCGAGGTGCTCGGCT CGCAGAGCTGAGCTCTCGAAGCAGAATGGCTCCTCGGCCTCTCAGATTTCTTCTGCTGAAGGCAGGGCAG CTGCAAAAGGTAACAACAGCTTGGAGAGGGAGAGGCAGAATTTACCAGCTCGTCCTAGCAGGGCTCCTGT GAGTATTTGTGGTGGTGGGGAAAACACCCCAAAGAGTGCCGAGGAACCGGTGGTGAGACCCAAAGTCCGG AATGTGGCGACTCCAAACTGCATGAAACCCAAAGTGTTTTTTGATACTGATGATGATGATGATGTACCAC ACAGTACTTCCAGGTGGCGGGATGCTGCCGATGCTGAGGAGGCTCATGCCGAGGGCCTAGCCAGAAGAGG CCGAGGTGAGGCTGCCAGCAGCTCAGAGCCAAGGTATGCTGAAGACCAGGATGCCAGGAGTGAACAAGCG AAGGCAGACAAAGTGCCAAGACGGCGGCGAACCATGGCAGACCCTGACTTCTGGGCATACACCGACGATT ACTACCGATACTACGAGGAAGATTCTGACAGCGACAAAGAGTGGATGGCTGCCCTGCGCAGGAAGTACCG AAGCCGAGAGCAACCCCAGTCCTCCAGCGGAGAAAGCTGGGAGCTTCTGCCAGGAAAGGAAGAACTGGAA CGTCAGCAAGCCGGAGCTGGGAGCCTCGCCAGTGCTGGCAGCAATGGCAGTGGTTATCCTGAAGAAGTAC AAGACCCATCTCTTCAGGAGGAAGAACAGGCCTCTCTGGAAGAAGGAGAAATCCCTTGGCTTCGCTACAA TGAGAATGAAAGCAGCAGCGAGGGTGATAATGAGTCTACCCATGAGCTCATACAGCCTGGGATGTTCATG CTGGATGGAAACAACAACCTGGAAGATGACTCCAGCGTGAGCGAAGACCTCGAAGTGGACTGGAGCCTGT TTGATGGGTTTGCCGATGGCTTGGGAGTGGCCGAAGCCATCTCCTACGTGGATCCTCAGTTCCTCACCTA CATGGCTCTGGAAGAGCGTCTGGCCCAGGCAATGGAGACGGCCCTGGCACACTTGGAGTCTCTCGCCGTT GATGTCGAAGTGGCCAACCCACCAGCAAGCAAGGAGAGCATTGATGCCCTTCCTGAGATCCTGGTCACCG AAGATCATGGTGCAGTGGGCCAGGAAATGTGCTGTCCTATCTGCTGCAGCGAATATGTGAAGGGGGAGGT GGCAACTGAGCTACCATGCCACCACTATTTCCACAAGCCCTGCGTGTCCATCTGGCTTCAGAAGTCTGGC ACCTGCCCAGTGTGCCGCTGCATGTTCCCTCCCCCGCTCTAAAAGCCAAGGCTCGTCGTAACAGTCAGCC TGGTTACATTCCCTGTCCGAAACCCACAATACTACAGGAGCCCTTGTTCTAAACTTACAATGAAACCAGT CAGTCAATTAGACTAAAGTTGTTGATTCCTTGTGATTATTTCCATGTGAAAATGGTTGTGTACAATGACA TTTAAAAAAAATCATCCTCTCGTTTAGAAGGTAGAAAGGGGGAAAGGAAACTTTCTAAATGCTGCTTGAG ATTGCAGTAAGAACATACATTTTCTAACCTGAAAGTTGAAACAAATCCCACTTGTTCTGTAGACTGTGTC TCTCTTACCTGTTGCTGTCAGGGTTACCTATCTGCTAAACTATGTCGGAAAGACAAAATTACTTTTGTTG CATGTCATGGGTTAATGTTCCTGTATTTGCAGTGGTGTAAAAGCTTATTAAAGTTCTTCTTTTGCTTTGA CACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC037616
Insert Size	1737 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC037616, AAH37616
RefSeq Size	2296 bp
RefSeq ORF	1737 bp
Locus ID	18744
Gene Summary	Has E2-dependent E3 ubiquitin-protein ligase activity. Ubiquitinates MAGED1 antigen leading to its subsequent degradation by proteasome. May be involved in protein sorting.[UniProtKB/Swiss-Prot Function]

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