Cntf (BC027539) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC41235
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC027539 CGCTTCGAGGGAAAAAAAAAAAGACTGATTAAAAGGGCCCTTTGATTCCCAGGCACAAAATCCACAGCCA GGAATTTGCTGCCACCTCTGATTCAGGCAGGGGGTGGGGATGGGGGTGCACAATCCCATTAGAGAATGCC CAGTAGATTTAGTCTGTGGGAGTCACATTTCTTATTTGGACTAGTGAAGACAGAAGCAAACCAGCTCACT TGTTTCCTGGGACAGTTGATTTAGGGGATGGCTTTCGCAGAGCAATCACCTCTGACCCTTCACCGCCGGG ACCTCTGTAGCCGCTCTATCTGGCTAGCAAGGAAGATTCGTTCAGACCTGACTGCTCTTATGGAATCTTA TGTAAAACATCAAGGCCTGAATAAAAATATCAGCCTTGACTCAGTGGATGGTGTACCAGTGGCAAGCACT GATCGCTGGAGTGAGATGACTGAGGCAGAGCGACTCCAAGAGAACCTCCAGGCTTACCGTACCTTCCAAG GGATGTTAACCAAGCTTTTAGAAGACCAGAGAGTGCATTTCACCCCGACTGAAGGTGACTTCCATCAGGC AATACATACTCTTACGCTCCAAGTTTCTGCCTTCGCCTACCAGCTAGAGGAGTTAATGGCGCTTCTGGAA CAGAAGGTCCCTGAAAAAGAGGCTGATGGGATGCCTGTCACAATTGGAGATGGTGGCCTCTTTGAGAAGA AGCTGTGGGGCTTGAAGGTCCTTCAAGAGCTTTCACAGTGGACTGTGAGGTCTATCCATGACCTCCGTGT CATTTCTTCTCATCACATGGGAATCTCAGCACATGAGAGCCATTATGGGGCCAAGCAAATGTAGCTCTTA ACTCTTGCCTCTGTCTCCCTCTTTGATCTAATGGGATATCAGTAGTTCCCCAGGGGCCTAGTTTTCTCAG CATCAATTTTGGAAAACCTTAGACCACAGGCATATTTCGTCAAGTAGGGTTGACCCACGTATGAACAGAA GATAAACATGGTTATTCTGGCTGTGCATATGTGTGTACATGATTTTGTTTGTATTGGTGGTATAATAAAG TATAATTAGGGCATTATCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC027539
Insert Size	597 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC027539, AAH27539
RefSeq Size	1106 bp
RefSeq ORF	597 bp
Locus ID	12803
Gene Summary	The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. A read-through transcript variant composed of Zfp91 and Cntf sequences has been identified, but it is thought to be non-coding. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse. [provided by RefSeq, Aug 2008]

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