Ada (BC002075) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC002075 GCCGCAGTCTCTGAGAGCCATCGGGAAGCGACCCCGCCAGCGAGCCAACGCAGACCCAGAGAGCTTCGGC GGAGAGAACCGGGAGCACGCTCGGAACCATGGCCCAGACGCCCGCATTCAACAAACCCAAAGTAGAGTTA CACGTCCACCTGGATGGAGCCATCAAGCCAGAAACCATCTTATACTTTGGCAAGAAGAGAGGCATCGCCC TCCCGGCAGATACAGTGGAGGAGCTGCGCAACATTATCGGCATGGACAAGCCCCTCTCGCTCCCAGGCTT CCTGGCCAAGTTTGACTACTACATGCCTGTGATTGCGGGCTGCAGAGAGGCCATCAAGAGGATCGCCTAC GAGTTTGTGGAGATGAAGGCAAAGGAGGGCGTGGTCTATGTGGAAGTGCGCTATAGCCCACACCTGCTGG CCAATTCCAAGGTGGACCCAATGCCCTGGAACCAGACTGAAGGGGACGTCACCCCTGATGACGTTGTGGA TCTTGTGAACCAGGGCCTGCAGGAGGGAGAGCAAGCATTCGGCATCAAGGTCCGGTCCATTCTGTGCTGC ATGCGCCACCAGCCCAGCTGGTCCCTTGAAGTGTTGGAGCTGTGTAAGAAGTACAATCAGAAGACCGTGG TGGCTATGGACTTGGCTGGGGATGAGACCATTGAAGGAAGTAGCCTCTTCCCAGGCCACGTGGAAGCCTA TGAGGGCGCAGTAAAGAATGGCATTCATCGGACCGTCCACGCTGGCGAGGTGGGCTCTCCTGAGGTTGTG CGCGAGGCTGTGGACATCCTCAAGACAGAGAGGGTGGGACATGGTTACCACACCATCGAGGATGAAGCCC TCTACAACAGGCTACTGAAAGAAAACATGCACTTTGAGGTCTGCCCCTGGTCCAGCTACCTCACAGGCGC CTGGGATCCCAAAACGACGCATGCGGTTGTTCGCTTCAAGAATGATAAGGCCAACTACTCACTCAACACA GACGACCCCCTCATCTTCAAGTCTACTCTAGACACTGACTACCAGATGACCAAGAAAGACATGGGCTTCA CTGAGGAGGAGTTCAAGCGACTGAACATCAACGCAGCGAAGTCAAGCTTCCTCCCAGAGGAAGAGAAGAA GGAACTTCTGGAACGGCTCTACAGAGAATACCAATAGCCACCACAGGCTGACGGGCAGGTCCCCTGAAGA TGGCAAGGCCACTTCTCTGAGCCTCATCCTGTGGATAAAGTCTTTACAACTCTGACATACTGACCTTCAT TCCTTCCAGACCTTGGAGAGGCCAGGTCTGTCCTCTGATTGGATATCCTGGCTAGGTCCCAGGGGCCTTG ACAATCATGCACATGAATTGAAAACCTTCTTTCTAAAGCTAAAATTATGGTGTTCAATAAAGCAGCTGGT GACTGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC002075
Insert Size	1059 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC002075, AAH02075
RefSeq Size	1464 bp
RefSeq ORF	1059 bp
Locus ID	11486
Gene Summary	Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine (PubMed:9272950). Plays an important role in purine metabolism and in adenosine homeostasis (PubMed:9272950, PubMed:10720488). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (PubMed:11435465). Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (By similarity). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (PubMed:9272950).[UniProtKB/Swiss-Prot Function]

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