Cyc1 (BC005620) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2610002H19Rik; AA408921; Cyct1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for BC005620, the custom clone sequence may differ by one or more nucleotides ATGGCGGCGGCGGCGGCTTCGCTTCGCCGGACGGTACTGGGCCCGCGGGGCGTGGGGCTTCCAGGTGCAA GTGCTCCGGGTCTGCTGGGCGGCGCGCGGTCCCGGCAGCTTCCATTGCGGACACCGCAGGCAGTGTCCTT GTCCTCGAAGTCTGGCCCTTCTCGAGGCCGAAAGGTGATGCTGTCAGCGTTGGGCATGCTGGCCGCGGGG GGTGCAGGGCTAGCTGTGGCCCTTCATTCTGCCGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCT ACCCATGGTCTCATCGTGGCCTCCTCTCCTCCTTGGACCACACCAGCATTCGGAGGGGTTTCCAGGTATA CAAGCAGGTGTGCTCTTCCTGCCACAGCATGGATTATGTGGCGTACCGCCACCTGGTGGGAGTGTGCTAC ACGGAGGAAGAAGCCAAGGCGCTGGCTGAGGAGGTGGAGGTCCAGGATGGCCCTAATGATGATGGGGAGA TGTTCATGCGGCCAGGGAAGTTGTCTGACTATTTTCCAAAACCATACCCTAACCCTGAGGCTGCAAGAGC TGCTAACAATGGAGCTTTACCCCCTGACCTCAGCTACATCGTTCGAGCTAGGCATGGTGGTGAGGACTAC GTATTTTCCCTGCTCACTGGCTACTGTGAGCCCCCCACTGGGGTGTCATTGCGAGAAGGCCTCTATTTCA ACCCTTACTTTCCCGGCCAGGCCATTGGCATGGCTCCTCCCATCTACACAGAAGTCTTGGAGTATGATGA TGGCACCCCAGCTACCATGTCACAAGTAGCTAAGGATGTCGCCACCTTCCTTCGCTGGGCATCAGAACCA GAGCATGACCATCGAAAACGCATGGGTCTCAAGATGTTGTTGATGATGGGCCTGCTGCTGCCCCTGACCT ATGCCATGAAGCGGCATAAGTGGTCAGTCCTGAAGAGTCGAAAGCTGGCTTATCGGCCACCCAAGTGA
Restriction Sites	EcoRI-NotI
ACCN	BC005620
Insert Size	978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC005620, AAH05620
RefSeq Size	1204 bp
RefSeq ORF	978 bp
Locus ID	66445
Gene Summary	This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain.[UniProtKB/Swiss-Prot Function]

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