Psmd14 (BC003742) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Poh1, Pad1, rpm11
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003742 CGGGCTGATTCATCGCCTGTTCTCCGAGCCAGGTCCTTGTTGAGTGTGAGGCGCTGCCCTCGCCGCCGCG TCGCCACAAGGCCAGGAGAGAGAGGAAAAACTACTTGTGAAAAATTGTATCTGCCAGAATTAGCAAGACT TGGAGTTAATAACAAATTTGTCAAATTCAACAAATTGAAGTTTAACACTGTAAGAATTACAGTTACTGAA CAGAAATATGGACAGACTTCTTAGACTTGGAGGAGGTATGCCTGGACTGGGCCAGGGCCCACCTACAGAT GCTCCTGCCGTGGACACAGCAGAACAAGTTTATATCTCTTCCTTGGCCTTGCTAAAGATGTTAAAACATG GTCGTGCTGGAGTGCCTATGGAAGTTATGGGTCTAATGCTTGGTGAATTTGTTGATGATTACACCGTCAG AGTGATTGATGTGTTTGCTATGCCACAGTCAGGAACTGGTGTCAGTGTTGAAGCAGTTGATCCAGTGTTC CAAGCCAAAATGTTGGATATGCTGAAACAAACAGGAAGGCCCGAGATGGTTGTTGGTTGGTATCACAGTC ACCCTGGCTTTGGCTGTTGGCTTTCTGGTGTGGATATCAACACTCAGCAGAGCTTTGAAGCCTTGTCGGA GAGAGCTGTGGCAGTGGTTGTGGATCCCATTCAGAGTGTAAAAGGAAAGGTTGTTATTGATGCCTTCAGA CTGATCAATGCTAATATGATGGTCTTAGGACATGAACCAAGACAAACGACTTCAAATCTGGGCCACTTAA ACAAGCCATCTATCCAGGCATTAATTCACGGACTAAACAGACATTATTACTCCATCACTATTAATTACCG GAAAAATGAACTGGAACAGAAGATGCTGTTAAATTTGCATAAGAAGAGTTGGATGGAAGGATTGACACTT CAGGACTACAGTGAACACTGTAAACACAATGAATCGGTGGTAAAAGAAATGTTGGAATTAGCCAAGAATT ATAATAAGGCTGTGGAAGAAGAAGATAAGATGACACCTGAACAGCTGGCAATAAAGAATGTTGGCAAGCA GGATCCCAAACGTCATTTGGAAGAACATGTGGATGTGCTTATGACTTCAAATATTGTCCAGTGTTTGGCC GCAATGTTGGATACTGTTGTATTTAAATAAAGTGACGTAGAGAGCTGTCAGTGGCGCTCTGAGTGTATCG TCCTTCTTCGTTCCCAATGCTCACAGTTAAGGGAACTGAAGGTGATCTGGTGATGTGTGAGACACCTGTG CCACCTCCCATCTCAGTTGTGCACTCGCTTCTGTTTATTTTGTCCGGGTTTTGCAGATTCCAAAGTTGTT CAGGAATACATCAAAGTGAACAAATTTTGTTAAGATCCCATTTACTATTTGAAAAAAATCAGTAGCACAA ATATATTTTGATTGTTGCTTACAAAATAAAATACATTTACAATCCAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC003742
Insert Size	933 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003742, AAH03742
RefSeq Size	1460 bp
RefSeq ORF	933 bp
Locus ID	59029
Gene Summary	Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. The PSMD14 subunit is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the complex. Plays a role in response to double-strand breaks (DSBs): acts as a regulator of non-homologous end joining (NHEJ) by cleaving 'Lys-63'-linked polyubiquitin, thereby promoting retention of JMJD2A/KDM4A on chromatin and restricting TP53BP1 accumulation. Also involved in homologous recombination repair by promoting RAD51 loading.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.