Gabarapl1 (BC004602) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MNCb-0091, GECI, Apg8l
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004602 CAGGCCGCGAGATCTCCAGGTCCTTCTCTGGACGTTTAGCCACGTATGCGGCGACTGGCGCGTGGGAGCT GACGCGGGTGGAGCGATTGTGATAAGGCTGACATTGACTGGGGGTGGCCAGACGCGAGACGCAGTCATCA GCGCGCGCAGCAGGCCCCGTCCGGGGAGCCCCTACATCCTGAGGTCCATCATGAAGTTCCAGTATAAGGA GGACCACCCCTTCGAGTATCGGAAAAAGGAAGGTGAAAAGATTAGGAAGAAATACCCGGACCGGGTGCCG GTCATCGTGGAGAAGGCTCCTAAAGCCAGGGTCCCTGATCTGGATAAGAGGAAGTACCTTGTGCCCTCCG ACCTCACTGTTGGCCAGTTCTACTTCTTAATCCGGAAGAGGATCCACCTGAGACCTGAGGACGCCTTATT CTTCTTTGTCAACAACACCATCCCTCCCACCAGTGCTACCATGGGCCAGCTGTATGAGGACAACCACGAG GAAGACTATTTTCTGTATGTGGCCTACAGTGATGAAAGTGTCTATGGAAAATGAGGCAGAAGCCCAGCAG ATGGGAGCACCTGGACTTGGGGGTAGGGGAGGGGTGCGCGTGGGACTTGGGGAACCAGAGGGAGGGCTCC CAACCATGGAGGAGACGCAAGGTGAAGACATCGGGAAACATCACACCGCACACGCTGTCGTCTTTTTTTC ACAAGCTCAATTGTTACTTTTCTCTGCTTCCTCAGCCCAGGAAGAACTTGTGTTGCATTGGCTGTGAGAG CGGGATGGGGACGACATGAATCACAGCTTTGCTGTGAGCTTTCTCTCGTGCAGTTTCATCAGAGGTTTGG GAGGAGGGCAGGTGGCCGCTAGAGAAGAATACAATTTAGCAGCAACTGCCGGCTCTTTGGGCAGAGGTTT GATTTTTGGCATTTGTACAAGCCGCACGGGCAAAGGGGGAAAAGAATGCCGTAGGGGACCACACCTAAGG GACCAAAAGATACCCACTGTCACTGAAGCATTGTACCCCTAACCTCTGTCTCCATACCTTCCTCTCCTAG TCCTTTTCTTCCACCCAGGCTTCATAGCTCCGCCCCAGTTGTGGCAGGAGACATTCGAACAGACGTTTAG ACGTTTAGAGTCCTCTTGGAAAATAGGGCCGTGCGGGAACGGTTATGATTGCCAGCGTTTTGGATGGCAT TAAACTGGAGTTCAGGGTTGAACATGTCCTGCTCCTCATCCCATAAGTACCTTGGACTTTCAGGAGCCTC AGGATCAGTCCTCTAGCATTTCCTTCACCTTTCTCATGTACAGGGGCAGCGTGTCTGTGTGTGGAGGGTC CGGGTTACAAGGTAAGCTAGGTTGCCCACTTGCCCACTCGTCCTCTCCCTGCTGCAGTCTCTTGGGTCTC ACACGCACTGCAAATGCTAAGCGATGGCCAGCTGTCTCCCTCCGTGGCATCTGCTATTCAAAGAAGATGG AGTGAGAGGGGCGCCAGAGTTTCATGGGGGCTTTACTCATTCATTTCATTTAGGGACTCTGTGGGGGGAG GGGCAGGCATTGCATTCTCACATGACATTTCAATTCATCTCTACCAATGAAGAGCGTCCTCCCTGTTGGG AGCAATCTGTACGTCTGTTTTGTCAGCTTCAGGTTCTGGTACTTTGAAGTCAATGCTGTCAGGCCAGGGA AATAAAATAATTGCTTACCTTAAAAGTCAAAAAAAAAAAAAAA
Restriction Sites	EcoRI-NotI
ACCN	BC004602
Insert Size	354 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004602, AAH04602
RefSeq Size	1723 bp
RefSeq ORF	354 bp
Locus ID	57436
Gene Summary	Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (By similarity).[UniProtKB/Swiss-Prot Function]

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