Ankrd32 (BC024900) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2700017A04Rik; Ankrd32; AW545819; Brctd1; Brctx; C730024G01Rik
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC024900 CTCAAGTTTTTCTTTAATTTACTTGAAGGTGAAGTTCGGCATTTGAGTCAAAAGTTATGTGACTGGTCAG ATTCTCAGAGTCTAAAAGTAACAGAAAAGGCAATTCTTCATGAAATTTTTTGGTCAGGAAGTGAAACTTC AGGACTTTTGACAAAACCAGTAAATATGCTTTTGGAATGGACTATATATTCTCACAAGGAAAAATGCAAG TCTAATGATGTCTTCAAACATGAACTATCTTACTTACTGACTGGAATTCTTGGTGCAGCAGTAGATTATT GGATCTTCCTTGGTATTCAGATGGGTAGAAATGTGATCCGACATATGTCTGATGACTTAGGAAGTTATAT TTCCCTTTCCTGTGATGACTTTTCTTCAAAAGAACTAGAGATCTTCATTTGTTCCTTTTCATCTTCCTGG CTTCAAATGTTTGTTGCAGAAGCAATTTTTAAAAAGCTGTGCTTACAGGGCCCCACTAGTACGTGCACTG AGCCACTCTCTCTTCAGAAAATAATAGATTCCTATTTGCCAGTTTTGGGAAAAATGGACATACATGGGGC TGGAAAGATGCAGAGTCCAAAAAAACTCTGCCAGCGGCCTTGCCTAGAGTCTCAAAGAGCATTACTAATG CTGAATGGTGCAAAACGGAAACAAGCAGAAGGTCGGCCAGAGCTCCTAGAGTTAAACCGTGCTAAATGTT CCTCATCATTGAAAAAATTGAAAAAGAAGTCAGAGGAATTGTCATGTTCCAAGGAGAATTGCCCATCTTT GGTTACAAAGATGAATTTTCATAAAACTAATCTGAAAGGGGAAACAGCTCTGCATAGAGTTTGCATAAAA AACCAAGTGGAGAAATTGATTATCCTTCTGTCTTTGCCAGGAATAGACATCAATGTTAAAGACAATGCTG GCTGGACGCCTTTGCATGAAGCCTGCAACTATGGCAACACGGAGTGTGTCCAGGAAATTCTGCAGCGTTG TCCAGAGGTGGATCTGCTCACTCAAGTGGACGGGGTGACGCCTTTGCATGATGCGCTGTCAAACGGACAT GTGGAGATTGGCAAGCTGCTTCTACAGCGTGGGGGCCCAGAACTTTTACAGCAGAGGAACTCTAAGGGGG AGTTGCCCTTGGATTATGTGCTGTCCCCTAAGGACAAGGAAGAACTATTTGCCATTACAAATATAGACGA TACAGTGGACAACTTTCATGCTAAGACACAGAAACATTTTTACCACCAACAGCTTGAATTTGGTTCGTTT TTACTTAGTAGGATGTTGATTAATTTTTGTTCAATATTTGATTTGTCCTCAGAATTTATCCTAGCTTTCA AAGGGTTAGGTCATCTAAATGAGTTGCTTATGGCATGCAATAGTGATACAGAAGCCAGCAATGCACATAC TGACTGGTTATTGGATGTTTATGCTAGGAATATAAAGACATTAAAGAAGCTCCCAAGTGTTCTTAAAGAG CTGCCTGAGAATCTAAATGTATGTCCTGGAGTGCATACTGAGGCCTTACTGGTGACACTGAAAATGATGT GTCAGTCAATCACAGAGCTGTCATGATGCCAGAAAGTATGAATCAGATGTCTGAACGTGCAACATGCTTT GTCATTTAGTATATCCATAGCTTATGGAAAAAATATTAATTTCAGTTACTCACTGACAGCAAAGTTTCAT AGCACTTATAAAACATCTACAGATCTAAAATATGGGCTCAGGATTTAACTCAGAAACATACAATAAGAAA ATGAAGTAGTTTTGTTCGGTATAAATGGTTTCACTGATTTTTAAAAAACAGATTTGTTCTTCAAATATTT ATTTATATTATATATATAGAAAATTAATTTAAATATTTTTTCAAGTCAAAATGTCCTTTCCTCTGAGTGC TACAGGTCTGAAAATCATGGCAAGGATATTCATTCAAATGTGCATCTATATAAATGTATAAAAGTTGTTT GTTCTTTATATTTAAGATGCATTTGCTTTTTTGTGTTTCTAAAATAAACCTTTCTAGGACTTTAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC024900
Insert Size	1266 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC024900, AAH24900
RefSeq Size	2075 bp
RefSeq ORF	1266 bp
Locus ID	105377
Gene Summary	Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of SLF2 and the SMC5-SMC6 complex to DNA lesions.[UniProtKB/Swiss-Prot Function]

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