Elovl5 (BC022911) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110059L23Rik; AI747313; AU043003; HELO1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC022911 CGCTTGCACATCCTCCTGCTCGCGCGGTCCTGCTGCCGTCCGGTGCTCCCCGTAGCCGCTCAGGCAGAGA GGTTTTAAAATGGAACATTTCGATGCGTCACTCAGTACCTATTTCAAGGCCTTCCTGGGCCCCCGAGATA CAAGAGTCAAAGGATGGTTCCTCCTGGACAATTACATCCCTACGTTTGTCTGTTCTGTTATTTACTTACT CATTGTATGGCTGGGACCAAAATACATGAAGAACCGGCAGCCGTTCTCTTGCCGAGGCATCCTGCAGTTG TATAACCTTGGACTCACCCTGCTGTCTCTCTACATGTTCTATGAGTTGGTGACAGGTGTGTGGGAAGGCA AATACAACTTTTTCTGCCAGGGAACACGCAGCGCGGGAGAATCCGATATGAAGATCATCCGCGTCCTCTG GTGGTACTACTTCTCCAAACTCATCGAATTCATGGACACCTTTTTCTTCATCCTTCGCAAGAACAACCAC CAGATCACCGTGCTCCATGTCTACCACCACGCTACCATGCTCAACATCTGGTGGTTTGTGATGAACTGGG TTCCCTGCGGCCATTCATATTTTGGTGCGACACTCAACAGCTTCATCCATGTCCTCATGTACTCGTACTA TGGTCTGTCCTCCATCCCGTCCATGCGTCCCTACCTCTGGTGGAAAAAGTACATCACTCAAGGGCAGCTG GTCCAGTTTGTGCTGACAATCATCCAGACGACCTGCGGGGTCTTCTGGCCATGCTCCTTCCCTCTCGGGT GGCTGTTCTTCCAGATTGGATACATGATTTCCCTGATTGCTCTCTTCACAAACTTCTACATTCAGACTTA CAACAAGAAAGGGGCCTCTCGGAGGAAAGAACACCTGAAGGGCCACCAGAACGGGTCTGTGGCCGCCGTC AACGGACACACCAACAGCTTCCCTTCCCTGGAAAACAGCGTGAAGCCCAGGAAGCAGCGAAAGGATTGAC AAGCCGAGCCGAAGCCTCCAGACCGCGGCGTGTGATTGTAAGCACAGCGTGACTTGTGCCCTGACGCTCA TAGCAGCTGCTGTCACTAGTCTGGCCCTACTATCTGCAGTGTGACTCATGTAGGACCTTCTTCATCAGTT CAAAACCCCTAGAATATGTATACAGATTATACAAAGTAGGCATACGATTTTCTCACATTTCTGGGCTCTC ATCGACCCCCGCGCTAGACTGTGGAAAGGGAATGTTCTTGTAGTATACAACACTGCTGTTGCCTTATTAG TTGTAACACGATAGGTGCTGAATTGTGGTTCACAATTTAAAACACTGTAATCCAAACTTTTTTTTTTTAA CTGTAGACCATGCATGTGATTGTAAATTTGTACAATGTTGTTAACAGTAGAAAAACACACATGCCTTAAG ACTTAAAAAAGCAGGGCCCAGAGCTTGTTAGTTTAATTAGGGTATGTTTCAACTTTGTATTATTCATTTG TAATAGCTCTGTTTAGAAAAATCAAATGTATGATTTATGAAACAAGTGTGTGATGTGTAACTTCAAGTGA CTTGGAGATGCGAAATCTGATGGCACCCTTGGTTTTATAACGATGGCTTTAAATAAGCAGGCTCAAGTCT AGTGGAACAGTCAGTTTAACTTTTTAACAGATCTTATTTTTTTATTTTGAGTGCCACTATTAATGTGTTT AAAGAGGTGGGGAAAGGGTCCAAAGCGTCTGGAGACACTTACGTACCTGTCCCTGGTCCCCCAGAGGCTA GGGTGACTATAAGAGTAGGTCACTTTTCACTTCTGAAGTGCTTAGTGTTTTTATACAGGAGATAAAAAGG CCATTCGACTTCCCACCACTTGGAGAGAGAGAACTACCTTTCAGGAAAGGTGACGAGACATTTATTATCT GACAGACACACACACACACACACACAGTGATAAGGGACTGGTTACCTGGGTCCACATTTTTTTTTATTCT TTTCTGTAGTGCTACTTTGACATACACTTCCTTTTAAAATGTGCCTAATTTACCAAGAGACCAGGAAGCA CCATAAGCATTTTGAGGTAAAATTTAAGAGCTGGAGCGCGCAGGAACTGGGTGTTTGGGAGCAACCGCTT TTTTCAGTGTGAATCATGGTGGCATCTGTAATTTTTTTCTGATTAGCACCTAAAGATCGGTTTAAAGTGC TTGGACTGCTTATCCTAAAATCGTGTCTAGTGCGCTCGAGGCATCGGTCATACAAATGGGGACTCTTTTC ATGTGTGTCATTTTTAAAGTGTTGAAAGCTTAGCCTTTGAATACCTTCTGCATTGGTAAAAAATCCATGG AACCACTCATGGCACATCTTAGGAGATGATTGACAATGTATAAACTAATTGTGGGTTTGATATTTATGTA AATACCAGTTTACCATGCTTTAATTTTGCACATTCATATTACAAGGAGCCAGTTGGTCCTAATTAGCCTT GTGGGTTTTCTGTTTGGAAGGAGTTGCGGCATCTGTTTACATTTCCCTTGTCAGATCTAGAATGTGTGTA TGTGTGTGTCTTCTCCCTAGGTACCCGTCTGCAGGTGTCTTTACATATCTCAATACAAAAATTATAACAT TTGATAGTGTGCTGTCAAAGTGTGCTTAGCTCATCTGGATATACCCACACTGTTAAATAATGTCTAAACA TTAATCATTAAAACCTTTTTGATTACCAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC022911
Insert Size	900 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC022911, AAH22911
RefSeq Size	2702 bp
RefSeq ORF	900 bp
Locus ID	68801
Gene Summary	Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity). In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid (PubMed:24184513).[UniProtKB/Swiss-Prot Function]

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