Akr1d1 (BC018333) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC25814
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC018333 TAGGCACCTTTTCAAATAATGTGCCTCTGCCCTGTTCAGAATCACCCCAGAAGCTTCAGATTCTTCTCTA CGATGAACCTCAGCGCTGCACACCACCAAATATCCCTAAGTGATGGGAACAACATTCCACTCATTGGGCT TGGAACCTACTCAGATCCTAGACCGGTCCCTGGGAAGACCTATGTGGCAGTGAAGACAGCTATTGATGAG GGGTACCGGCACATTGATGGCGCCTATGTTTACCACAATGAGCATGAAGTCGGTGAGGCCATCAGAGAAA AGATAGCAGAAGGGAAGGTAAAGAGGGAAGAGATCTTCTACTGCGGAAAGTTATGGAATACAGAGCATGT CCCATCAATGGTCCTCCCAGCCCTGGAAAGGACGCTAAAGGCCCTCAAACTAGATTACATAGACCTTTAT ATTATCGAACTGCCCATGGCCTTTAAGCCTGGAAAGGAAATTTACCCTAGAGATGAAAATGGCCGAATTA TATATGACAAAACAAATCTGTGTGCCACGTGGGAGGCATTGGAAGCTTGCAAAGATGCTGGCTTGGTGAA ATCCCTTGGGGTGTCTAATTTTAACCGCAGGCAGCTGGAGCTCATCTTGAACAAGCCAGGACTCAAGTAC AAACCAGTCACTAACCAGGTGGAGTGCCACCCGTATTTCACCCAGACAAAACTCTTGAAATTCTGCCAGC AGCATGACATCGTCATTGTCGCACATAGCCCCTTAGGGACCTGCCGCAACCCATCATGGGTGAACGTTTC TTCTCCACCCTTGTTAAATGACGAACTCCTAACCTCACTGGGAAAAAAGTACAATAAGACACAAGCTCAA ATTGTGTTGCGTTTCAACATCCAGCGAGGGATAGTTGTCATTCCTAAAAGCTTTACCCCCGAAAGGATCA AAGAAAACTTTCAGATCTTTGACTTTTCTCTCACGGAAGAAGAAATGAAGGACATTGATGCCTTGAATAA AAATGTCCGCTATGTGGAGTTGCTCATGTGGAGTGACCATCCTGAATACCCATTTCATGACGAATACTGA ACATGGAAATTTCTTCAACAGAGTTTTTTTTTTTCTTCATTCAAGATCTTAAGCTGATTAATCCACTCCA GATGTAGATAAATGGGGTCTTGCTCTCTTCAGATTAAGATGGGCAGAACACACCATGCTTAACTTTTGTG TAGTATTAACTCAGCTAGCTCTGATGAAGAGAAAAGAAGATTCATCTTTATTTAATCTATCTGGATAGTT CTTTGCAAATGTCTTACTAATATCTTTAGATCAATGTCTTCTAGACTGTTCATGGAAATCACTAAACTTT ACTCAAAAGTAGCATGTAAAAGCAGGTCCAAGAATCTGAGACTAATATAGGGAGTCTGATTTGTTTAACC TTTAGCAGGACAAAGAGGTTTGCAGTTGGCACGGTTCCTGGTGCTGACCATGACCATGACCTTCCAAATG TTTGGACCATTAGTTGCAAGATGGTCACAGCCAAGGCAGAAATCCACATGCCATTCCTAATGCACCTTGA GTAGAGATAGAACTGGAAGTCTTGAAAAGTCCTAAAGAAGAACATACTGTAGGATGTTCCAGGGCTAGAG GTTCCTGCAGTGCTTGCCTGGCAGGCTGGAGGCCCAGGGTGAGGGAGGTGGGGATGAGGGCAATCGGCAA AGCCATGCTGAGTCTGTTTTTATCATTTGTTTCCATCCAGTGTGCCCATTCAGCCTTTCATGTGCGTGAA GGGTGGACAACACTGATTCTTTCTTAAATTGTTTTTGTTTAGGAGGGTGGGGAGTTGAGACAGGGTCTTG TGTAGTCCAGACTGCTCACAAACCTGCTGTGCAGTTGAGGCCAGTCTTGAATTCTGAATCCCTGGTCTTT CTCCCATTTTTAATGCCCTGAGCTTTCATGTGACTGGTAAGCCCTACTTGTACTATGTTATAATCATTCT TGCCTGCACTCATCAAGAAAACCACCATGTAAAGTGGCAAGAAACAAGATATACAAATCAAGCTTTGTAC CATAAAATCGTCTGAAGATGGTGATTTTTTTTTTAAAAAATTATTTTATGTATATGAGTGCACTGTAGCT GTCTTCAGAAACACCAGAAGAGGGCATCAGATCTCACTACAGATGGTTGTGAGCTACCACGTGGTTGCTG GGATTTGAACTCAGGAAGTTTGGAAGAGCAGCCTGTGCTCTTAACCGCTGAGCATCTTTCCAGCCCCCAA ATGATTTTTTTTTCTTTTTTAAAGAAGGATTTGAGAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC018333
Insert Size	978 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018333, AAH18333
RefSeq Size	2291 bp
RefSeq ORF	978 bp
Locus ID	208665
Gene Summary	Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one).[UniProtKB/Swiss-Prot Function]

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