Fgf15 (BC021328) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	FGF19
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC021328 CCACGCGTCCGGTGCGCCCCACGCCACAAGCGCCCAGTTGCTTTGTGGGTTGTGCCTGCCCTGCGCCTGC AACTTGAGTCCCCGCCGCATCGCAGTCTCCGCGCCACCTTTGTAACGGCCTTCAGGACCCCGAGGTGTCA TGGCGAGAAAGTGGAACGGGCGTGCGGTGGCCCGAGCCCTGGTCCTGGCCACTCTGTGGCTGGCTGTGTC TGGGCGTCCCCTGGCCCAGCAATCCCAGTCTGTGTCAGATGAAGATCCACTCTTTCTCTACGGCTGGGGC AAGATTACCCGCCTGCAGTACCTGTACTCCGCTGGTCCCTATGTCTCCAACTGCTTCCTCCGAATCCGGA GCGACGGCTCTGTGGACTGCGAGGAGGACCAAAACGAACGAAATTTGTTGGAATTCCGCGCGGTCGCTCT GAAGACGATTGCCATCAAGGACGTCAGCAGCGTGCGGTACCTCTGCATGAGCGCGGACGGCAAGATATAC GGGCTGATTCGCTACTCGGAGGAAGACTGTACCTTCAGGGAGGAAATGGACTGTTTAGGCTACAACCAGT ACAGATCCATGAAACACCATCTCCATATCATCTTCATCCAGGCCAAGCCCAGAGAACAGCTCCAGGACCA GAAACCCTCAAACTTTATCCCCGTGTTTCACCGCTCCTTCTTTGAAACCGGGGACCAGCTGAGGTCTAAA ATGTTCTCCCTGCCCCTGGAGAGTGACAGCATGGATCCGTTCAGGATGGTGGAGGATGTAGACCACCTAG TGAAGAGTCCCAGCTTCCAGAAATGACAGGATTCCGACAGGATGGAGAAAACCCCAAGGTCCCGTGAACT TCCCCCTTAGGAAGCTGTACATATTCTAAGTCTCACATGGACCCTGTTGTGTTAGTGGCTAGACTTGATC ATGAACCTAAATTGACAACCTGCCTGGCTGCCATCGGAGCCCCACTGACTTTGGAGGCTGCTGATATGTG CCTAAGTTACTCCAGTTCTGTTTGAATACCTCCACTAATAGGGAACTTACTCCTGTGAAACATTCTTAGT TTTGAGCCAAATCTGTGACTTGGATGGTTTTAGCGAGGAAGCCAGAAGGTATGAAGTCAAATGATAAAAT TCATGTATAGAAAGTGGGCTCTAAAATATATATTCCCTATATGGATCTCATGGGATCTTAGCTTGCCCCC CAAATGTCTCCTGGCCAGAACTAACTGGGGTTACAAAACTTGGAACAAAGGACAGCCTAGAAAACTTTGG GAGCCTTGAAGGATGGTCTTAGGATTACGAATTCCAGCTGACTACGTAGCTTCCCCCTTTTCCACTTATA AATGTCAGATGGAAGTGACCCTTAGCTGAGTGCATAGCCAAGCTGCCACTTAGGCCCCAGGAGCTTGTCT CTGTCCCATGACCCCAGATTTCCAGGACCTGGATCTTCTCCTCTGACCTTTCCCAGAGTTCACCTGGGCT CTCCAACCCCAGAGCAGGTAGCTTATGAGCCATCCAGTTGTGTCCCCAGCTCCTGGCTCTCAGTTCTGGT CACCAAACATTGTGAATCAACGTGTCTGCTGCCTGTGTCAACCTGGACCCCTCATTTACAAACAAGATTA GGAAGCCCCAAATTCTCCAGTGGCAGTTGGGGAACTGTGGAGTCCTTTCCCCGGCACTTACGTGGCAGCA TGATATTTATAAGTAATTTATTGTGTGTGTGTCTTCTATTTTCTTACTTTATTTATGCCCCAGATCATAT TTATGTACATGACTTGTTTTCTACATTAAAAAGGAGTTGGTTTGTAAAAAAAAAAAAAAAAAAAAAAAAA A
Restriction Sites	RsrII-NotI
ACCN	BC021328
Insert Size	657 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC021328, AAH21328
RefSeq Size	1821 bp
RefSeq ORF	657 bp
Locus ID	14170
Gene Summary	Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.