Aldob (BC036132) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC36398
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC036132 GCCACCTCACACAGCTTCTGATACCTTGGAGGACTCTTCCCCTTTGCTGTACCTGTCATCATGGCTCACC GATTTCCAGCCCTCACCCCAGAGCAGAAGAAGGAGCTCTCTGAAATTGCCCAGCGCATTGTTGCCAATGG GAAGGGCATCCTGGCTGCAGATGAATCTGTGGGTACCATGGGAAACCGCCTGCAAAGGATAAAGGTGGAG AACACCGAAGAGAACCGAAGGCAGTTCCGAGAACTCCTCTTTAGTGTGGACAATTCCATTAGCCAGAGCA TCGGCGGAGTGATCCTTTTCCACGAGACCCTCTACCAGAAGGACAGCCAGGGAAATCTGTTCAGAAACGT TCTCAAGGAGAAGGGAATTGTGGTGGGCATCAAGTTGGACCAAGGAGGTGCCCCGCTTGCAGGAACAAAC AAGGAAACCACCATTCAAGGGCTTGATGGCCTCTCTGAACGCTGTGCTCAGTACAAGAAAGATGGAGTTG ACTTTGGGAAGTGGCGTGCTGTGTTGAGGATTGCTGACCAGTGTCCTTCCAGCCTTGCTATCCAAGAAAA TGCCAATGCTCTGGCTCGCTATGCCAGCATCTGTCAGCAGAATGGGCTGGTCCCTATTGTTGAGCCAGAG GTGCTTCCTGACGGAGACCATGACCTGGAGCACTGCCAGTATGTTTCTGAGAAGGTCCTGGCTGCTGTCT ACAAGGCTCTCAATGATCATCATGTTTACCTAGAAGGCACCTTGCTAAAGCCAAACATGGTGACTGCTGG ACATGCCTGCACGAAGAAGTATACACCAGAGCAGGTGGCTATGGCCACTGTCACAGCTCTCCACAGAACT GTTCCTGCAGCTGTTCCTGGTATCTGCTTTTTGTCTGGAGGTATGAGTGAGGAGGATGCTACACTTAACC TCAATGCTATCAACCGTTGCCCTCTACCAAGGCCCTGGAAACTAAGCTTTTCATATGGAAGAGCCCTCCA GGCCAGTGCATTGGCTGCCTGGGGTGGCAAGGCTGCAAACAAGAAGGCAACCCAGGAAGCTTTCATGAAG CGGGCTATGGCTAACTGCCAGGCGGCCCAAGGACAGTACGTTCACACAGGCTCTTCAGGTGCTGCTGCCA CCCAGTCGCTCTTCACAGCCTCCTACACCTATTAGATACTGTCACCACCAGCCAGGCTCCAGTTCTCCTA AATATTTTGGAGACCTGAATGGCTCAGTCAACCCTGGAAATTAGATAACATTTGATTGAACTTCTGAAAA CACAACACATGTGAATCATAAACACAAGAGAAAAGCCAAATCAGCTTTGAAACCCAAGAATGAGCATGTC ACCCAATAGTCTCTCAACACTTCCAGTTCCCTATGTTCCAGAACATTCATCCAAGAAAAGAACAGGCCAC GACTGAGAAAAGCAACTCAGAAGCAGAGGATGGAGAAGGGCATTTTAATAAACATAGTTACCAGGGGTTG GTTATAGAAGAGGCACAGCAACAGAAAAAGAAATAAAACGTGTATGAACCTTCCGTTGGTACAGTTTCAC TTTCCTGCTCTTGTTGAGGGATCCTGCATTTCATTGTCTTTGCCTATACCAGATACAACCCCAATAGTCA GGATCCTCAGCCCCAGATGCTCACTACAGTCAAGTGGCTGATGGCTCCCTTTAGCTGAATTACACTAGAA ACTCTACCAGATTAAATAAGAGTTCTTTTGGGGCTTCCTATATGCAGTCATACTTGAGAACAGTCAGTCT AGACTTCTAGCTTGAGAATATTTGAGAATACTAAGCTGCAACAGAGACTCATCTCTTCCAAGCTATGGAG TGTAGAGAATGAGTTGATAAACCTGACTTGGCATACTCCTCTCCAGGAATCCAGGTTTTGTGACACAGAA CTACATTTTCCTCTCAAAGTCAAGCTGGTCCCCCAGCTGTGCAATAAACAATCGTTGAATAATAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC036132
Insert Size	1095 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC036132, AAH36132
RefSeq Size	1993 bp
RefSeq ORF	1095 bp
Locus ID	230163
Gene Summary	This gene encodes a subunit of the homotetrameric enzyme aldolase B, an isozyme of the class I fructose 1,6-bisphosphate aldolase enzyme. This enzyme catalyzes the conversion of fructose 1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Homozygous knockout mice for this gene exhibit liver damage and death following fructose ingestion. A pseudogene of this gene has been identified in the genome. [provided by RefSeq, Aug 2015]

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