Slc30a7 (BC017136) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Zntl2, ZnT7, ZnT-7
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC017136 GTCTGTGTCTGTGTCTCGCGGCGGTGCCGCGGCGGTCAAGCGCTTTGGGGTCCGCGTTTGAAGCGTCACC GCTGTCACTGCCGCCACCATACCGAGTCACTTCTAGAGGGACGGAGACCCGGCCCTTCGCCGGGTGGAGA AGATGTTGCCCCTGTCCATCAAGGATGATGAATACAAACCACCCAAGTTCAATCTGTTCGGCAAGATCTC AGGCTGGTTTAGGTCCATCCTGTCGGACAAGACATCCCGGAACCTGTTTTTCTTCCTGTGCCTGAACCTC TCTTTCGCTTTTGTGGAACTACTCTACGGCATCTGGAGCAACTGCCTAGGCTTGATCTCCGACTCCTTCC ACATGTTTTTTGATAGTACCGCCATATTGGCCGGATTGGCAGCTTCTGTTATTTCAAAGTGGAGAGATAA TGACGCTTTCTCTTATGGGTATGTTAGAGCAGAAGTCCTGGCTGGCTTTGTCAATGGGCTGTTTTTGATC TTCACTGCTTTCTTTATTTTCTCAGAAGGAGTCGAGAGAGCATTGGCTCCTCCAGATGTGCACCACGAGA GACTGCTGCTTGTTTCAATTCTTGGATTTGTGGTAAACCTAGTAGGAATATTTGTTTTCAATCATGGAGG TCACGGACATTCTCATGGCTCTGGCCATGGCCACAGTCATTCCCTCTTTAATGGTGCTCTAGATCACAGC CATGGCCACGAAGATCATTGCCATAGTCACGAAGCCAAGCACGGAGCTGCGCACAGCCATGACCATGACC ACGCTCATGGCCATGGGCACCTGCATTCCCACGATGGCCCATCCTTCAAAGCAACGGCAGGACCCAGCAG ACAGATTTTACAAGGTGTATTTTTACACATTCTAGCCGATACACTGGGAAGTATTGGTGTAATTGCCTCT GCCATCATGATGCAAAATTTTGGGTTGATGATAGCAGATCCTATCTGTTCGATTCTTATAGCCATTCTTA TAGTTGTAAGTGTTATTCCACTTTTAAGAGAGTCTGTTGGAATATTAATGCAAAGAACTCCTCCCTCGTT GGAAAATACGCTTCCCCAGTGCTATCAGAGGGTGCAACAGTTACAAGGAGTTTACAACTTGCAAGAACAG CACTTCTGGACACTCTGTTCTGATGTTTACGTGGGAACCTTGAAGCTCGTGGTAGCCCCTGATGCTGACG CCAGGTGGATCTTAAGCCAAACGCATAATATTTTTACTCAGGCTGGAGTGAGACAGCTTTATGTACAGAT TGACTTTGCGGCAATGTAGTCAAAGGAAGGAAACTGATTGAGGACCAAACTTTCCCAGTACTGTGCAGTC CAAAGCATTGTACTACCCAGTGCTCTGAAACAGAGACACGACCACTACAACTCAGAAGCACCAAGGACGC AGGTAGAGTCAGCCGCGTGTTCGAGCGGTGTCACAGCCGGATCCTGCCCAGACTGCTGCCTGTGTGCTTT CCCTTCAAGCCATTCTGACTGCTGAGGGTTCTGGGTTCCTTCTAGGGTATTGGGTTATCTTTCCCCGCTG AGGGTTCTTGCTGATCACACTCACCCCATCCTTATCATCCCACCCAGCCAGTGAGAAATTCAGACTGGAG GCCTTCAACCTTCTGAATGTCTTCACTAAAGCTGCTGGAAACCACTGCTTTTATTCCCATAAAACCAGTG TTGTTTTTACAAAAAGAAGTCTGTTTTCTATGTAATGTCACAGTTGTTGACGTTCTTCAGTGTAATCATA TGTTTGTAGAAATAGTTTGTACCTTGCAAACTTACAAACCAAACCATATTGGGTTTTCAAAGTGCCTTTG TATCAGAAATATATTTGCCAGCATAGAAATGTACCATCAAAGGTCATGTGTATGTTTTTTTAAAAATGGG TATTCTGTAATTTGTACAAAATAAGACTTGCTAGTAACATACATAATTGTCTTTGTGTATTTCCAACATA GATTTATTTTGCTTTATAAAAAATCCAGTTATCACATTAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC017136
Insert Size	1137 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC017136, AAH17136
RefSeq Size	2015 bp
RefSeq ORF	1137 bp
Locus ID	66500
Gene Summary	Seems to facilitate zinc transport from the cytoplasm into the Golgi apparatus. Partly regulates cellular zinc homeostasis. Required with ZNT5 for the activation of zinc-requiring enzymes, alkaline phosphatases (ALPs). Transports zinc into the lumens of the Golgi apparatus and the vesicular compartments where ALPs locate, thus, converting apoALPs to holoALPs. Required with ZNT5 and ZNT6 for the activation of TNAP (By similarity).[UniProtKB/Swiss-Prot Function]

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