Rnaseh1 (BC019411) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC019411 CGCGCTGGCGTGAGTGATGCGCTGGCTGCTGCCGCTGTCCCGCACAGTGACACTGGCCGTCGTACGCCTG AGGCGAGGCATTTGCGGGCTCGGGATGTTCTATGCGGTGAGGAGAGGCCGCAGGACCGGAGTCTTCCTGA GTTGGAGTGAGTGCAAAGCCCAGGTGGACCGGTTTCCTGCTGCCAGGTTTAAGAAATTTGCCACAGAAGA TGAGGCCTGGGCCTTTGTCAGGAGCTCTTCAAGCCCGGATGGTTCAAAAGGGCAGGAAAGTGCACATGAG CAGAAGTCACAGGTGAAGACCAGCAAGCGGCCTCGGGAGCCCCTGGGTGAAGGGGAAGAACTTCCAGAGC CAGGGCCAAAGCACACACGACAGGACACCGAGCCCGCTGCTGTAGTGAGCAAGGACACGTTTTCTTATAT GGGAGAGTCAGTCATTGTCTACACGGATGGCTGTTGCTCCAGTAATGGACGGAAGCGGGCACGAGCAGGA ATTGGCGTTTACTGGGGCCCAGGCCACCCCTTAAATGTAGGTATAAGGCTTCCTGGGCGACAGACAAACC AGAGGGCCGAGATCCATGCAGCCTGCAAGGCCATCATGCAAGCCAAGGCTCAGAACATCAGCAAGCTGGT TCTGTACACAGACAGCATGTTCACCATCAATGGGATAACTAACTGGGTTCAGGGCTGGAAGAAGAATGGC TGGAGAACAAGTACAGGGAAAGATGTGATCAACAAGGAGGACTTCATGGAGCTGGACGAGCTCACTCAGG GCATGGACATCCAATGGATGCACATTCCTGGTCACTCAGGATTTGTGGGCAATGAAGAGGCCGACAGACT GGCACGGGAAGGAGCGAAGCAGTCTGAGGACTGAGCAGAGGAGCTGCTGCCGACTCAGACCCAAGGCAGA GGCTGTTTCTTTACCCTGCCGTGCACTGAGCTGTGCATGGAGGACACAGTGTAACCTGTGGCACAGCTTG GTGAGACGTGCTTACATAATGGAAAGGAAGTGTTAAATCCTGCTTTTGTGAGATTTCAGGGTCTGTCCCA AAATGCTCCAGCTGGATTTTAGTAGGAGTCAGTGAGACCCTGGTCAGTACGTGTGGCCTAAAAGTTCCAG AAGGCACAAGTGCAAGACTCCTGCCCCTGCAGCTGCCCTCTATCTCTGTGCATCTGAGATAATGTGGCCA CATGGGACACAGCCTACACTCAGGTGGCCTTGACTCCTGGATCACCTTGAAGGAACAGCTGAGACCTTTA AAAATCAATATATTACTAATAGATTTCTTTGCCTATTTTTTCTTAAAAATACTGTATAAATTTAAAACAT GTTGAAATTATCCTTATTTATTTTTTATAATAAAAGTATATAGGAATGACACAGAAAAAAAAAAAAAAAA AAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC019411
Insert Size	858 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC019411, AAH19411
RefSeq Size	1409 bp
RefSeq ORF	858 bp
Locus ID	19819
Gene Summary	This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which encodes 7aa in mouse. An alternately spliced transcript variant has been found which is a candidate for nonsense-mediated mRNA decay (NMD). [provided by RefSeq, Nov 2013]

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