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Plod1 (BC006599) Mouse Untagged Clone
CAT＃: MC206271
Plod1 (untagged) - Mouse procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 (cDNA clone MGC:11398 IMAGE:3965301), (10ug)
CNY 5,344.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Plod, LH1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC006599
GCGGGTTGTTCCCGAGACTCCGCTATGCGGTCCCTGCTGCTCCTGGCCCCGTTGGCCTGGCTTCTTCTGG TCCAGGCGAAGGATGACGCCAAGCTAGAGGACAACCTTCTGGTCCTCACTGTGGCCACAAAAGAGACTGA GGGCTTCCGCCGCTTCAAGCGTTCAGCTCAGTTCTTCAACTACAAGATCCAGTCACTGGGCCTGGGGGAG GACTGGAGTGTGGATGGAGGACCAGCAGCAGCAGGTGGAGGACAGAAGGTTCGGCTGCTGAAGAAGGCAC TGGAAAAGCACGCAGACAAGGAAGACCTGGTTATTCTCTTCGTTGACAGTTATGACGTGGTGTTTGCCTC GGGACCCCGGGAACTCTTAAAGAAGTTCCAGCAGGCCAAGAGCCAGGTGGTCTTCTCCGCAGAGGAGCAT ATCTACCCGGACCGGAGGCTGGAGGCCAAGTATCCCACAGTCCCCGACGGCAAGCGGTTCCTGGGCTCTG GAGGCTTCATTGGTTATGCCCCCAGCCTCAGCAAACTGGTGGCCGAGTGGGAAGGCCAGGACAGTGACAG CGACCAGCTCTTTTATACCAAGATCTTCTTGAACCCGGAGAAGAGGGAGCAAATCAATATCAGCCTGGAC CATCGCTGCCGAATCTTCCAGAACCTGGATGGAGCCTTGGATGAAGTTGTGCTCAAGTTTGAAATGGGCC ACGTGAGAGCACGAAATCTGGCCTATGACACCCTCCCAGTGGTCGTTCATGGCAATGGGCCCACCAAGCT GCAGCTGAACTACCTGGGCAACTACATTCCTCGATTCTGGACCTTCGAGACGGGCTGCACCGTGTGTGAC GAGGGCCTACGGAGCCTCAAGGGCATTGGGGATGAAGCTCTCCCCACTGTCCTGGTCGGTGTGTTCATTG AGCAGCCCACACCATTTCTATCCTTGTTTTTCCTGCGGCTGCTGCGCCTACGATACCCTCAGAAACAGAT GCGGCTCTTCATTCACAACCAGGAACGGCACCACAAACTTCAGGTAGAGCAGTTCCTGGCGGAGCACGGC AGTGAGTACCAGTCTGTGAAGCTAGTGGGTCCTGAGGTGCGCATGGCCAACGCCGACGCCAGGAACATGG GCGCAGACCTGTGCCGTCAGGACCAAACTTGCACCTACTACTTCAGTGTGGATGCTGATGTGGCTCTGAC GGAGCCGAACAGCCTAAGGCTCCTGATTGAACAAAACAAGAATGTCATTGCGCCACTCATGACCCGCCAC GGGAGGCTCTGGTCCAACTTCTGGGGGGGTCTGAGTGCAGATGGGTACTACGCCCGCTCAGAGGACTACG TGGACATTGTGCAGGGCCGTCGTGTTGGCGTCTGGAACGTGCCTTACATCTCAAACATCTACCTGATCAA GGGCAGTGCCCTGCGGGCTGAACTGCAGAACGTAGACCTTTTCCACTACAGCAAGCTGGATTCCGACATG AGTTTCTGCGCCAATGTCCGACAGCAGGAGGTGTTCATGTTCCTGACCAACCGGCACACCTTTGGCCACC TGCTTTCCCTGGATAACTACCAGACCACCCACCTACATAATGATCTCTGGGAGGTGTTCAGCAACCCTGA GGACTGGAAAGAAAAGTACATCCATGAGAATTACACCAAGGCCCTGGCGGGGAAGCTGGTGGAGACGCCT TGTCCGGATGTCTACTGGTTCCCCATCTTCACGGAGGCGGCCTGTGATGAGCTGGTGGAGGAGATGGAAC ACTATGGCCAGTGGTCTCTGGGTGATAATAAGGACAACCGGATCCAGGGTGGCTACGAAAACGTGCCCAC TATCGACATCCATATGAACCAGATCACCTTCGAGCGGGAGTGGCACAAGTTCCTGGTGGAGTACATCGCC CCCATGACAGAGAAGCTGTACCCTGGCTACTACACTAGGGCCCAGTTTGATCTAGCCTTCGTCGTCCGCT ATAAGCCCGATGAGCAGCCTTCCTTGATGCCCCACCATGACGCCTCTACCTTCACCGTCAACATAGCCCT GAACAGGGTTGGGGAAGATTATGAGGGCGGAGGTTGCCGATTTCTGCGCTACAACTGCTCCGTGAGGGCA CCAAGGAAGGGCTGGGCCCTCCTGCACCCCGGGCGGCTCACACACTATCACGAGGGGCTTCCTACTACCA AGGGCACGCGCTACATTGCTGTGTCTTTCGTCGATCCCTAACTGCCCAGTGCCTGCCTCTGTTCGACCTA TCTCATCGTTGACAACCACTGCCCAGCAGCCCTTGGGGCCAAGGAACCCAATCCAACCTCTGATTCCTGA ACTCCTGTAGCTCTTGAAGCCACCAGTTGGAACGATGGGGTGCAGGCCAGAGATGGGGCACCCACTCCAG GACTGGGGCTGCCCATGGTGCTCCCACTAATCTCAGGAGATGGTAACGCTCATCTTCAAGGAGTTCCCAG CCCCCCTTTTTCCTCTGACTTTCCCTGGGGCCAGACTTCGACGGAGCTGCCTGGGATGCTGGAGAGTCAG AAGCTTTGCCTAGAGCAGCTCTGGAAGGGCTAGCCCCCAGGATTCTGTTTCAAGTATCAGTGCCACCAAG GTCTGGGTCAACCCACCTACCCCAACTCCACCCCGAACCTAGGACTCTTGATGCCCCTCTCTTTCAGGTT TTTATCACCAAGAGCAAATAGTTGTCTCTATTGAGACTGTAACTCGAAATCCTCCCGGGGGGCAGGAAAG ACATCTGTGACAATGCTTGGTTCTCTACTGACCCTGCTTGGGGGAGGGGCATATGTCCACACTGTACTGG GTCACCCTGTCCCGATGCCTCTGGAGACAGGGACAGAGACGAGAGTTCTATTAAATGTCGTTCAAACCAC AAAAAAAAAAAAAAAA |
| Restriction Sites | EcoRI-NotI |
| ACCN | BC006599 |
| Insert Size | 2187 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006599, AAH06599 |
| RefSeq Size | 2886 bp |
| RefSeq ORF | 2187 bp |
| Locus ID | 18822 |
| Gene Summary | Part of a complex composed of PLOD1, P3H3 and P3H4 that catalyzes hydroxylation of lysine residues in collagen alpha chains and is required for normal assembly and cross-linkling of collagen fibrils (PubMed:27119146). Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens (By similarity). These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links (PubMed:27119146).[UniProtKB/Swiss-Prot Function] |
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