Mapk15 (BC048082) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC56865, MGC56903
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC048082 CGCGGCTGCTGGTTCCCAAGCCAACGGATCAACAGGTCCAGCTCCAGAGAAAGAATCGGTGGACCCCTAG AGCCAGCGCCTGCCTCTCGCCATGTGTGCTGCCGAGGTGGACCGTCATGTAGCCCAGAGATACCTGATCA AGCGGAGGCTTGGGAAGGGGGCCTATGGCATTGTGTGGAAGGCCATGGACCGGAGGACTGGCGAGGTTGT GGCCATCAAGAAAATCTTTGATGCCTTTAGGGACCAGATAGATGCTCAGAGGACCTTCCGTGAGATTATG CTTCTCAAGGAGTTTGGAGGCCATCCCAACATCATCCGCCTGCTTGATGTAATCCCAGCGAAGAATGACA GGGATATTTACCTGGTGTTTGAGTCCATGGACACCGACCTGAATGCAGTCATCCAGAAGGGCAGACTACT GAAGGACATCCACAAGCGGTGCATCTTTTACCAGCTCCTGAGAGCCACCAAGTTTATCCATTCAGGGCGC GTCATCCATCGGGATCAGAAGCCAGCCAATGTTCTACTGGATTCTGCTTGCCGGGTGAAACTCTGTGACT TTGGCCTGGCACGCTCCCTCGGTGACCTCCCTGAGGGGCCTGGGGGTCAGGCCCTGACAGAGTATGTGGC CACACGCTGGTACCGAGCTCCAGAGGTGCTTCTGTCTTCCCGATGGTATACCCCTGGGGTGGACATGTGG AGCCTGGGCTGCATATTAGGAGAGATGCTTCGAGGGCAGCCACTGTTTCCCGGGACATCTACTTTCCACC AGCTGGAGCTGATCCTGAAGACCATCCCATTGCCTTCCATGGAGGAGCTCCAGGACCTTGGCTCAGACTA CAGTGCTTTGATTCTGCAGAATCTTGGGTCCAGGCCACAGCAGACGCTGGACGCCCTCCTGCCGCCAGAC ACCCCCCCAGAAGCCCTGGACCTCCTCAAGCGACTCTTGGCGTTTGCTCCGGACAAGCGCCTTAGTGCAG AGCAGGCGCTGCAACACCCCTACGTGCAGAGATTCCATTGCCCCGACCGCGAGTGGGCACGGGAGTCCGA CGTGCGGCTCCCGGTGCACGAAGGAGACCAGCTCTCTGCACCAGAGTATCGCAAACGCCTGTACCAGATT ATCCTGGAGCAAAGTGGGAACAGCCGCAGCCCTCGAGAGGAAGGCCTGGGGGTTGTGGCCTCGCGGGCTG AGCTCAGGGCTTCCCCGGCCCGGACGCAATCGCTCAAGTCGGGAGTCCTCCCCCAGGTCCCGGCGGAGAC GCCAGCGCGAAAACGCGGACCCAAACCTCCGCGTAGCCCTGGTCATGATCCTGAGCATGTGGAAGTTCGC AGGCAGAGCTCAGACCCCCTGTTCCAACTTCCGCCGCCAGGAAGGGGGGAAAGGCCCCCAGGGGCCACAG GGCAGCCACCCTCGGCACCCTCAGGGGTGAAGACTCAAGTGAGGGCGATGGCGCCGTCCCTGACTTCACA GGCAGAGGCTCAGGCGGCCAATCAGGCTCTGATCCGCAGTGATCCGGCCCGGGGCGGTGGGCCGAGGGCG GTCGGCGCGCGACGGGTCCCTTCCCGCCTGCCCCGGGAGGCCCCGGAACCCCGACCCGGCCGAAGGATGT TTGGCATCTCGGTCTCGCAGGGGGCCCAGGGCGCAGCCAGAGCTGCGCTTGGCGGCTACTCCCAGGCCTA CGGGACCGTGTGCCGCTCGGCGCTGGGCCGCCTGCCCCTGCTCCCCGGACCGCGTGCGTGAGCCACCCAC CCACCTCCTTGAGGCAACCCGGCGCCGTCCCCCAGACCCCAATGCATCTTCTCCCTCCTCTGCCTCCCTG CCTTCGAGTTCCCGGAGGGTTCTTTGAGCCTCTCTCCCCTAAGAAAGAGTAGATGGAAGTCTCCACTCCA CCCCCCAATACTTTCTTCCAATAAAGTTTTATCTTTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Restriction Sites	EcoRI-NotI
ACCN	BC048082
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC048082, AAH48082
RefSeq Size	1961 bp
RefSeq ORF	1650 bp
Locus ID	332110
Gene Summary	Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner (By similarity) (PubMed:25823377). Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3' UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression (By similarity). Also functions in a kinase activity-independent manner as a negative regulator of growth (By similarity). Phosphorylates in vitro FOS and MBP (By similarity). During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos (PubMed:23351492). Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity (By similarity).[UniProtKB/Swiss-Prot Function]

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