Ndufs1 (BC006660) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MGC7850, MGC19199
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC006660 CGTCCTCCAGGTCGGGCTGACAGAGGTTGCCGTGGCCGCCATATTGACTTAGCGGCCAGGGTTTTGAGGG GTCAGGGAGACCCGGACAGGTCCGCGGAACTACTCGGTGGGCTCCGGAGGAAAGCCATCATGTTAAGGAT ACCTATAAAAAGGGCCTTGATAGGCCTTTCTAATTCTCCTAAAGGATATGTTCGCACAACTGGCACAGCA GCAAGTAACTTGATTGAAGTATTTGTTGATGGTCAGTCTGTCATGGTGGAACCAGGAACCACTGTTCTGC AGGCTTGCGAGAAGGTCGGCATGCAAATCCCTCGATTCTGTTACCATGAAAGGTTGTCTGTTGCTGGAAA TTGCAGGATGTGCCTGGTAGAGATTGAGAAAGCTCCAAAGGTTGTCGCTGCTTGTGCTATGCCTGTAATG AAGGGCTGGAATATCTTGACAAACTCGGAAAAATCTAAGAAAGCCAGAGAAGGTGTGATGGAGTTCTTAT TAGCAAATCACCCATTGGATTGTCCTATTTGTGACCAGGGAGGTGAATGTGATCTGCAGGACCAGTCCAT GATGTTTGGAAGTGATAGGAGCCGATTTCTAGAGGGGAAGCGTGCTGTGGAGGACAAGAACATTGGGCCC CTAGTAAAGACCATCATGACTAGATGCATCCAGTGTACCCGGTGCATCAGGTTTGCAAGTGAGATTGCAG GAGTAGATGATTTGGGAACAACAGGAAGAGGAAATGACATGCAAGTTGGCACATACATTGAAAAAATGTT TATGTCTGAACTGTCTGGGAATGTCATTGATATCTGCCCTGTAGGGGCCCTAACCTCTAAGCCTTATGCC TTTACTGCCCGGCCTTGGGAAACAAGAAAGACAGAGTCCATTGATGTAATGGATGCAGTGGGAAGTAACA TTGTGGTTAGCACAAGAACTGGAGAGGTAATGAGGATTTTGCCAAGAATGCATGAAGATATTAATGAAGA ATGGATCTCTGATAAAACCAGGTTTGCTTATGATGGACTAAAACGTCAAAGACTTACTGAACCAATGGTC AGAAACGAAAAAGGGCTTTTAACTTATACCTCCTGGGAAGATGCACTCTCTCGTGTAGCTGGAATGTTAC AGAATTTTGAAGGCAATGCTGTGGCAGCGATTGCAGGAGGCTTGGTGGATGCTGAAGCCTTAGTAGCTCT GAAAGACTTGCTTAATAAAGTTGACTCTGACAACTTATGCACTGAAGAGATCTTCCCCACTGAAGGAGCT GGTACAGACTTACGTTCCAATTATCTTCTCAATACCACAATTGCTGGTGTGGAAGAAGCAGATGTTGTTC TTCTAGTTGGTACAAATCCACGTTTTGAGGCACCGCTGTTTAATGCTAGAATTAGAAAGAGCTGGCTTCA TAATGACCTAAAAGTGGCCCTCATCGGTAGTCCAGTGGACCTCACTTACAGATACGACCATCTAGGAGAC TCTCCTAAAATTCTGCAAGACATTGCTTCAGGGAGGCATTCATTCTGCGAGGTCTTAAAGGATGCTAAAA AACCAATGGTGGTTTTAGGCAGTTCTGCACTCCAGAGAGATGATGGAGCAGCAATTCTTGCAGCTGTGTC CAACATGGTACAAAAGATTCGAGTGACAACCGGTGTTGCTGCAGAGTGGAAAGTTATGAATATTCTGCAT AGGATTGCAAGCCAGGTAGCTGCTTTGGACCTTGGCTATAAACCTGGGGTAGAAGCGATTAGGAAGAACC CTCCCAAAATGCTGTTTCTTCTGGGAGCAGATGGAGGTTGTATCACGCGACAGGACTTGCCAAAGGATTG TTTCATTGTTTATCAAGGACACCATGGTGATGTTGGTGCTCCCATGGCTGATGTTATTCTCCCAGGGGCT GCTTACACAGAAAAGTCTGCTACTTATGTCAATACTGAGGGCAGAGCTCAGCAAACCAAAGTAGCAGTGA CGCCTCCTGGCTTGGCAAGAGAAGACTGGAAAATCATAAGAGCTCTCTCTGAGATTGCAGGTATCACTCT TCCATATGATACTCTGGATCAAGTAAGGAACCGTCTTGAAGAGGTCTCTCCTAATCTGGTTCGATATGAT GATATTGAAGAAACTAATTACTTTCAGCAAGCAAGTGAGCTTGCCAAGCTAGTAAACCAGGAGGTTCTTG CTGACCCACTCGTTCCACCTCAGCTAACTATAAAAGACTTCTATATGACAGACTCAATTAGCAGAGCCTC ACAGACAATGGCCAAGTGTGTCAAAGCTGTCACTGAGGGCGCTCAGGCAGTAGAGGAGCCGTCCATATGC TGAGACATCTACCAGGGACCCTTTTTGCTACAGACAGTAAATGGACATTGCTGTAATCCCTTATAAGTTA ATGTTTTCCAACATGAGTCTGAATGATGTAATATTTAAAGTTATACTATGCTTATCCGAAAATGGTATTG TACTTATCAAAAATCCTGGCAGTTTAAAATGTATGTAATTGTGTGCAAGCATTAACTAGTTTAATAAAAC TATCATTTTCTCTTTCATGTTAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC006660
Insert Size	2184 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC006660, AAH06660
RefSeq Size	2564 bp
RefSeq ORF	2184 bp
Locus ID	227197
Gene Summary	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).[UniProtKB/Swiss-Prot Function]

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