Mre11a (BC065144) Mouse Untagged Clone

CAT#: MC206210

Mre11a (untagged) - Mouse meiotic recombination 11 homolog A (S. cerevisiae) (cDNA clone MGC:86077 IMAGE:30627207), (10ug)



CNY 5,176.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Mre11b, Mre11
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC065144
CTGGGAGCGGTTTTCTTGTGGTTCTGGAGACTCCCGCCCCCTTTTCCTGAGAGTTTGGATTCGAGTGGTT TTTCTGACATACCTGAACACTTGGAGAGGAACCTGGTACAGAGGAACTCTACTATAAAAATGAGCCCCAC AGATCCACTTGACGATGAAGACACATTTAAAATCCTGGTTGCCACTGATATTCATCTTGGATTTATGGAG AAAGATGCAGTTAGAGGAAATGATACATTTGTGACATTTGATGAAATCTTAAGACTTGCCCTGGAAAATG AAGTGGATTTTATTTTGTTAGGTGGTGATCTTTTCCATGAAAACAAGCCCTCGAGGAAAACCCTGCACAG CTGCTTGGAGCTGCTTAGGAAGTACTGTATGGGTGACCGCCCTGTGCAGTTTGAGGTCATCAGTGATCAG TCAGTCAACTTTGGTTTTAGTAAGTTTCCATGGGTGAACTACCAGGATGGCAATCTCAACATTTCCATTC CAGTATTTAGTATCCACGGCAACCATGATGATCCCACGGGGGCAGATGCCCTCTGTGCCCTGGATGTTTT AAGCTGTGCTGGGTTTGTGAATCACTTTGGACGGTCAATGTCGGTGGAGAAGGTTGACATTAGTCCGGTT CTGCTGCAGAAAGGAAGCACAAAACTCGCTCTGTACGGCTTAGGCTCCATTCCAGATGAAAGGCTCTATC GGATGTTTGTGAATAAAAAAGTAACAATGTTGAGACCAAAGGAAGATGAGAACTCATGGTTTAACTTATT TGTGATTCATCAGAACAGGAGTAAGCATGGAAACACCAACTTCATTCCAGAGCAGTTTTTGGATGACTTC ATCGACCTCGTTATCTGGGGCCATGAACATGAGTGTAAAATTGGCCCAATCAAAAATGAGCAGCAGCTCT TCTATGTGTCTCAGCCCGGAAGCTCAGTGGTGACGTCCCTTTCCCCTGGAGAAGCTGTGAAGAAACATGT AGGCTTGCTGCGCATTAAAGGGAGAAAGATGAACATGCAGAAGCTGCCTCTCCGCACCGTGCGGCGGTTC TTCATAGAAGACGTGGTTCTGGCTAACCACCCAAACCTGTTCAACCCTGACAATCCTAAGGTGACCCAGG CCATCCAGAGCTTCTGTCTGGAGAAGATTGAAGAAATGCTTGACAGTGCTGAGCGGGAACGACTGGGGAA TCCTCAGCAGCCGGGGAAGCCTCTTATCCGACTACGGGTGGACTATAGTGGAGGCTTTGAACCTTTCAAC GTTCTTCGTTTTAGCCAGAAGTTTGTGGATCGAGTCGCTAACCCAAAAGATGTCATCCACTTTTTCAGGC ACAGGGAACAAAAGGGAAAAACAGGAGAAGAGATCAACTTTGGGATGCTCATCACAAAACCTGCTTCAGA AGGAGCAACGCTTAGAGTAGAAGACCTCGTAAAGCAGTATTTCCAAACTGCAGAGAAGAATGTTCAGCTC TCGCTGCTGACAGAAAGAGGGATGGGCGAAGCAGTTCAAGAGTTTGTGGACAAGGAAGAGAAAGATGCCA TCGAGGAATTAGTGAAGTACCAGCTGGAGAAAACACAGCGGTTTCTTAAGGAGCGCCATATTGATGCTCT GGAAGACAAGATTGATGAGGAGGTCCGACGTTTCCGAGAAAGCAGACAGAGAAATACCAACGAAGAAGAT GATGAAGTTCGAGAGGCCATGAGCAGAGCCCGGGCCCTCAGATCACAGTCAGAGACCTCCACCTCAGCCT TTAGTGCTGAGGACCTGAGCTTTGATACATCGGAGCAGACAGCAAATGACTCTGATGACAGCCTGTCAGC AGTGCCGAGCAGAGGCCGAGGCCGAGGCCGAGGGCGAAGAGGAGCCAGAGGGCAGAGCTCGGCACCTAGA GGAGGCTCTCAGAGAGGCCGAGACACTGGGCTGGAGATCACTACTCGAGGCAGGAGCTCAAAGGCCACCT CATCAACATCTAGAAACATGTCCATTATAGACGCTTTCAGATCTACCCGACAACAGCCTTCTAGAAATGT AGCCCCTAAGAATTACTCAGAGACCATTGAGGTGGATGACTCTGACGAAGATGACATTTTTCCTACCAAT TCCAGGGCTGATCAAAGGTGGTCGGGCACAACATCTAGCAAACGGATGTCCCAGAGCCAGACAGCCAAAG GGGTAGACTTTGAATCAGATGAGGATGATGACGATGACCCTTTCATGAGCAGTAGTTGCCCAAGAAGAAA CCGAAGATAATGTTTTTAATGGAACTTAGAAATTGCATGATGCAGGAAGAAATCAAAGCACCACATACTG ACTTCACAGCTGAAGATGTTTAAGTCTGGGCTTATATTTTATCGTTTTCTGAACGGTGACTCCCTTAAGC CATTTCCTAGCCCAATGATAAGGAGATTGCAGCTCTTCTTAGCCTTTAACCTGGTTGTTATTGTCTGGGG CTGCTCTGCCATATCGAGAACATTAACTTCCTGACAGAACTGTGTTTTCTGCTTGTAAGAACTTGGCTAA AATCCCATTCTTAAATGATGATTAGAGAAAACCCCTCCGTTGTTCATTTCTGCTTCATGATCAGAGGCTG TCAGCCTCTGTGCCACAGCTAGCAGTTGATGGGAAAGCTTACAGACTTGAGTGGCATGTGCTGTAACCAT CTCCTAGATCTCCAGAAAGCCAGGCCCTCCTGTGTGGGGGGCACCCAGCACCCTGCTTGTTATCAAGTGT GGATATCAGAGCTTGCTAGAGGACCGTCTACTAGGACCAGAAGGGTCTTATATTTAAATAATTAGTAGAA GGTTTTTGTTAAGAACATTTTGGCTGTTTTGGTTTTGATTTTTATTCATGTTATAGCAGAGATTCAGATC CTGTGCCCATTGGGCAGGACTCATTTTCTTGATGGCTCTGTCAGTAATGACAAGCTCTTTTTCTTTCTCG TATACTTGTCAGGAAGAAAAGGCTAATAGAATTTTTCTGTTTTCTTAAAAATACCGGAAAGCTTATAGAC TTAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI     
ACCN BC065144
Insert Size 2121 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC065144, AAH65144
RefSeq Size 3034 bp
RefSeq ORF 2121 bp
Locus ID 17535
Gene Summary Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...