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Polr3b (BC068143) Mouse Untagged Clone
CAT＃: MC206192
Polr3b (untagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (cDNA clone MGC:93030 IMAGE:30362434), (10ug)
CNY 7,896.00
货期*
现货
规格
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2700078H01Rik; A330032P03Rik; C85372; RPC2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC068143
CTGCCGACTTCTTCCTCTACCGTGAATGGAATGGATGTTCTAGCCGAGGAGTTCGGGAGCCTGACTCCGG AGCAGCTGACTGCGCCGATCCCGACTGTGGAGGAAAAATGGAGGCTGCTTCCAGCATTTTTAAAGGTTAA AGGCCTGGTGAAACAGCACATAGACTCCTTTAACTATTTCATTAATGTAGAGATAAAGAAGATAATGAAA GCCAATGAAAAGGTTACAAGTGATGCTGACCCCATGTGGTACTTAAAATACCTTAATATCTATGTCGGAC TTCCTGATGTTGAAGAAAGCTTCAATGTAACTAGACCAGTGTCCCCTCATGAGTGCCGTCTGAGGGACAT GACGTACTCCGCCCCAATCACAGTGGACATTGAGTATACCCGAGGCAGCCAGAGGATAATCCGCAACGCC TTACCCATTGGCAGAATGCCCATCATGCTCCGAAGCTCGAACTGTGTTCTAACAGGGAAAACGCCGGCAG AGTTTGCCAAACTGAATGAGTGTCCACTAGACCCAGGTGGCTACTTCATTGTTAAAGGAGTGGAAAAAGT CATTCTTATCCAAGAGCAGCTGTCCAAGAACAGGATCATTGTGGAGGCCGACAGGAAAGGAGCCGTTGGA GCCTCAGTTACCAGCTCCACCCATGAGAAGAAAAGCAGAACCAACATGGCCGTGAAACAGGGTCGATTCT ATCTGAGGCACAACACCCTGTCTGAAGATATCCCCATTGTCATCATATTTAAGGCCATGGGTGTGGAGAG TGACCAGGAAATCGTGCAGATGATTGGAACAGAGGAGCACGTGATGGCTGCATTTGGGCCCAGCTTGGAG GAGTGCCAGAAAGCTCAGATCTTCACCCAGATGCAGGCATTGAAGTATATAGGGAATAAAGTAAGAAGAC AAAGAATGTGGGGAGGTGGACCAAAGAAAACCAAAATAGAAGAAGCAAGAGAACTCCTGGCTTCTACCAT TCTGACCCACGTACCAGTGAAGGAATTCAATTTCCGAGCTAAGTGTATCTACACTGCAGTGATGGTGCGA AGGGTGATCCTGGCCCAAGGAGATAACAAGGTCGATGACAGAGACTATTATGGCAACAAACGACTGGAAT TGGCAGGACAGCTCTTATCTCTTCTTTTTGAAGATTTGTTTAAAAAGTTTAATTCAGAAATGAAGAAGAT TGCAGACCAAGTGATTCCTAAACAGAGAGCAGCCCAGTTTGACGTCGTTAAACACATGCGCCAAGACCAG ATTACCAACGGCATGGTCAATGCCATCTCCACGGGAAATTGGTCTCTGAAGAGATTTAAGATGGACCGAC AGGGTGTGACCCAGGTGCTGTCTCGCTTGTCATATATATCTGCACTCGGCATGATGACAAGAATCTCTTC CCAGTTTGAAAAGACGAGGAAAGTGAGTGGCCCTCGTTCTCTGCAGCCGTCTCAGTGGGGAATGCTCTGT CCCTCCGACACACCTGAAGGGGAGGCCTGTGGTTTGGTTAAGAACTTGGCCCTCATGACACATATAACAA CTGACATGGAAGATGGACCCATTATTAAGTTAGCTGGGAACTTGGGAGTAGAAGATGTGAATTTATTATG TGGAGAAGAGCTCTCTTACCCAAATGTGTTTCTTGTCTTCCTGAATGGTAACATCCTTGGTGTCATTCGA GACCATAAAAAGCTTGTCAGCACATTCCGGCTGATGCGAAGAGCAGGCTATATCAATGAATTTGTTTCTA TCTCCACAAACCTTACGGATCGCTGTGTTTATATTTCCTCTGATGGCGGGCGCTTGTGCAGGCCTTACAT AATTGTTAAGAAACAAAAGCCAGCTGTCACAAATAAGCACATGGAAGAGTTGGCTCAAGGCTACAGGAAT TTTGAAGATTTCTTACATGAAAGTCTAGTTGAATACTTAGATGTGAATGAAGAAAATGACTGTAACATTG CGCTCTACGAACATACAATTAATAAAGACACCACCCACTTGGAGATTGAACCCTTCACTCTGCTTGGTGT TTGTGCCGGCCTGATCCCATACCCTCATCACAACCAGTCTCCAAGAAACACCTACCAGTGTGCTATGGGC AAGCAAGCCATGGGTACCATTGGCTACAATCAACGGAACAGAATTGATACACTTATGTATCTACTAGCAT ATCCACAAAAGCCCATGGTTAAAACAAAAACAATCGAGTTGATAGATTTTGAGAAGTTGCCGGCTGGCCA GAACGCAACTGTTGCCGTGATGAGCTACAGCGGCTATGACATTGAGGACGCGCTGGTTTTGAACAAGGCC TCTCTGGACAGAGGTTTTGGGCGTTGTCTTGTGTATAAAAATGCTAAGTGCACCTTGAAACGATACACCA ATCAGACCTTTGATAAGGTGATGGGTCCCATGTTGGATGCTGCTACCAGAAAACCCATCTGGCGACATGA AATTTTAGATGCAGATGGCATTTGTTCTCCAGGTGAGAAAGTGGAAAACAAACAGGTGCTTGTAAACAAG TCCATGCCCACAGTGACACAGATTCCTTTGGAAGGGAGCAACGTGCCACAGCAACCACAGTATAAAGATG TGCCTATCACGTACAAAGGTGCGACAGACTCCTACATCGAGAAAGTGATGATCTCTTCCAACGCCGAGGA TGCTTTCCTCATCAAGATGCTCCTGAGACAGACGCGGCGCCCAGAGATTGGAGACAAATTCAGCAGTCGC CATGGACAAAAGGGTGTCTGTGGCCTGATCGTCCCCCAGGAAGACATGCCGTTCTGTGATTCTGGCATCT GTCCCGACATCATAATGAACCCACACGGCTTCCCATCAAGAATGACGGTTGGAAAGTTAATTGAGCTGCT GGCTGGCAAGGCAGGCGTGTTAGACGGCAGGTTCCACTACGGCACTGCCTTTGGAGGGAGTAAAGTGAAG GATGTGTGTGAAGATCTCGTTCGCCATGGTTACAACTACCTGGGGAAGGACTACGTCACCTCAGGCATCA CAGGTGAGCCCTTAGAAGCATACATCTATTTTGGACCTGTGTATTATCAGAAACTGAAACACATGGTGCT GGATAAAATGCACGCCCGGGCCCGAGGACCACGAGCTGTCCTTACCAGGCAGCCCACTGAAGGGCGGTCG CGCGATGGTGGCTTGCGACTTGGAGAAATGGAACGCGACTGTCTAATCGGCTATGGAGCGAGTATGCTTT TGCTGGAGAGACTAATGATCTCAAGTGATGCCTTTGAGGTTGACGTCTGCGGGCAGTGTGGACTCCTGGG ATATTCTGGCTGGTGCCACTACTGCAAATCTTCGTGCCACGTGTCCTCCCTCCGCATCCCGTATGCCTGC AAGCTGCTCTTCCAGGAGTTACAGTCCATGAACATCATCCCCAGGCTGAAACTGGCCAAGTACAACGAGT AAGGCTGTGCGGTGCTCACGAGGGTGCAGTCGGCCGACGGCCTGTACAGCCTGAGGCAGGGGGCTCTCGG TGACTTCTGTGCTCATTCTGCTTCACGGAGGAGGCAGAGGAGGAGGAGGAAGAAGAGGAAGAAGGAGAGG AGGAGGAAGAGAATGAAGGAGAGGAGGAAGAGGAGCAGCAGCAGCAGCTGTCTGAGGTCCAGAGGCTGGC TTGACACCCTTGATCTGGAGCCTGAAGCCATAGGAGGTGCTGGGCCCCGCACAGCACTGAGTGGAGTCAC ACACAGATGGACACAAGCTAGAGAGAGTACCAACGCCTATTAATTTTGTTGTTTGTTTGTTTGTGTTGAG TGGGAGTCTCACTCTATCCCAGGTGGGATTTGAACTCACCTCAATTCTCCTGCCTTTGTCTCCCACAAGT GCTGGAATCATGGATATGAGCCAAGCATATCTGGTTTGTTTATGTTATTATGTTTTAAACTGTACTTGTT AGAAAAACCTTCTTCCTTTAAATTTCCTCTGAGGTCTTCTAGCTAACAGAATCCTGGGTTCTCATCAGAT GTGGAGGAGGTAAAGAATTGTTTGAATTGGGACTGAGAAGTTAGCTCAGTCAAGGGCCTGTTGCATAAGT ATGACCTGAGTTCACCCCCCAACAACCCATGTAAGGGCTGGGTGTGGCACCTAGCACCCCTGTAAAAGTC AGATACTGAGGGGGTGGGGGTGGGGAGCAGACAGACTGACCCCTGGCGCTTTCTGACCAGCTGGCATAAC CAGTCAGTGCAATTCAGGTTCAATGAGGGACCCCTGTGTCAAGGGCTAGTGTGAACAGTGACTGAGGAGG ACCCCAGTGTCCCTCTGGCCTCCACACACACATCCAAAGAATGGGTAGATGTGTGGGCACAACTTTTGCT GAGGTGGGAAGAAGTAAACTTTCTCTCAGAGGACAGCAAGTGACATTTGTGACTCTGAAAGACCAGCAGT CACAGTCTGCTGTCACATTGTGAGTCAGAGCTTGCTCAGACATCGATCTTGATGGAATGTGATCTGTGAA GTCTACCTTTTAAGACTCTCCTCCTACTTCTTTTGAACCTGCAGGATACCTAGGTCTAATCCATGGGCCC CTGGTCACTCATTTCCTACCTTTCCATTGCTACCAGAAAGTTAGCTGTCCACTCTCTCAGTTTGTTCTTA ACTGTTGTGCCCTGGCTCCAGATAAATGTTTATATGGAGAAGTGTTCAGAGAGAGAGTGTGCCCCAGCAG CATTGAGTGTGCGGATGAGGCTGAGGAGGGAGTGCGAGGACTTTGCAGATCTCACTCCAGGCTCATCCGG ATGAGTCACGACTTAAAGACTCAACCCTGTTTGTTTTCTTCACAATAAAGATGTGGCAGTCAGAACGGTA AAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | BC068143 |
| Insert Size | 3402 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068143, AAH68143 |
| RefSeq Size | 4858 bp |
| RefSeq ORF | 3402 bp |
| Locus ID | 70428 |
| Gene Summary | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity).[UniProtKB/Swiss-Prot Function] |
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