Ccnd2 (BC049086) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2600016F06Rik; AI256817; BF642806; C86853; cD2; Vin-1; Vin1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC049086 CGAGCTGAGGAGAGCCGGGCAGTTCGGAGGGAAGGACCGGTGCGAGTCAGGCGGCCCTTGAGGCTCCGCT CGCCCACCTTCCACTCTTCTCTCTCTCTCTCCCTCTCTCTCTTTGCCATTTCTTTCCTCTCCCAAATCTC CCATTCAGCCAAAGGAAGGAGGTAAGGGAAGCACTCCCCGACCCCCCCGCACCTCCAAAAAATAATAATA ATAAAAAAAATTTACAGTCGGGACCGAGTGGTGGCCGGCTGGCTATGGAGCTGCTGTGCTGCGAGGTGGA CCCGGTCCGCAGGGCCGTGCCGGACCGCAACCTGCTGGAAGACCGCGTTCTGCAGAACCTGTTGACCATC GAGGAGCGCTACCTCCCGCAGTGTTCCTATTTCAAGTGCGTGCAGAAGGACATCCAACCGTACATGCGCA GGATGGTGGCCACCTGGATGCTAGAGGTCTGTGAGGAACAAAAGTGTGAAGAAGAGGTCTTTCCTCTGGC CATGAATTACCTGGACCGTTTCTTGGCTGGAGTCCCGACTCCTAAGACCCATCTTCAGCTCCTGGGTGCA GTGTGCATGTTCCTAGCTTCCAAGCTGAAAGAGACCATCCCGCTGACTGCGGAAAAGCTGTGCATTTACA CCGACAACTCTGTGAAGCCCCAGGAGCTGCTGGAGTGGGAACTGGTAGTGTTGGGTAAGCTGAAGTGGAA CCTGGCCGCAGTCACCCCTCACGACTTCATTGAGCACATCCTTCGCAAGCTGCCCCAGCAAAAGGAGAAG CTGTCCCTGATCCGCAAGCATGCGCAGACCTTCATCGCTCTGTGCGCTACCGACTTCAAGTTTGCCATGT ACCCGCCGTCGATGATTGCAACTGGAAGCGTGGGAGCAGCCATCTGTGGGCTTCAGCAGGATGATGAAGT GAACACACTCACGTGTGATGCCCTGACAGAGCTGCTGGCCAAGATCACCCACACTGATGTGGATTGTCTC AAAGCCTGCCAGGAGCAAATCGAAGCTCTGCTGCTGAACAGCCTGCAGCAGTTCCGTCAAGAGCAGCATA ACGCCGGATCCAAGTCTGTGGAAGATCCGGACCAAGCCACCACCCCTACAGACGTGCGGGATGTTGACCT GTGAGGAAGCCATTCGGGCGGCAAGAGAGAGGCGTGTTCGTCATCTGCTAGCCCCTTCTCTCTCTAGTTA TGTCTTGTTCTTTGTGTTTTTAGGATGAAACTTCAAAAAAGAAAAAAAAAAAACAAAAAACAAAAACAAA ATCTGCCCCCACCTAGATCCTATTTAAAGGTCTTTTAGAAGTGAGAGGAAAAGGCCGTATAGTTGTAACG CATGTGGTGCCTGTTCAAAGTCCTGCATAAGGGGATCCCTGTACACTCGAACCGTTATTATTTGATGATG TAAAAGTCATAGTGAGATGCTTACAGGAGAACCCGCAGACTAGCTAGAGAGTGTGCGTACCTGCAACATG GGAACGAATTAGAGGAGACTGTCTTTTGTGCTTGTGACCTAGTGCATATGCCCCCTTTAGTAGAATTGCA AGGAACCGTGGGTGCCAGGACCGCATGAGTAGTTTGCAAAGCAGTGAACCCAAGGAGGAATCAGAAACGA GAAGGGACATGCCGGGGAGGTTGCGCAAAACCACCCAACCACATGACTGAACCATTTTGGATGTAAGAAG CGCCTTGATGCCTCACCACGTGTTCCCAAGACTGAGCGTCCGGGTCTCTCCGTCGCATACTCTTGCTGAC TAAAGTAGCTGCTACCTAAGGGATATGTCTTTTGCCAGTTGGACACAGGTGAACGGCTCCTAAGTCTCAT GTTTGGTGAATTTGGGCTTCTACTTCCAAACATGGTTCATTGTAGATGTATTTTACTGTTATCTGATGGA GGGAATTCGCTATGGGCCATGACCAAAACGCATCTAAATGGGGGCAGATGGAGACCATAGACGGAATCTA AAACAAAGCTGCCTTGTGTAAGGGAAGAGGGTGAGGTGATCTTTAATCCCCTTGTTAAGAAAAAAAAAAA AAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC049086
Insert Size	870 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC049086, AAH49086
RefSeq Size	2037 bp
RefSeq ORF	870 bp
Locus ID	12444
Gene Summary	Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).[UniProtKB/Swiss-Prot Function]

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