Gpr12 (BC055746) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Gpcr01, Gpcr20
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC055746 CGGCATGGGAGATGCAATTAGCCAATGTCGGTTTTCAGCGTTTTGGCAAGTGTGCGAGTGTGCATGTGCC GCCTCGGGAGTCCTGATCCGTGTTTCCCTCAGAGACAAACAGCATTTCGGTTGCAGACTTTAGCTTTTGT TTTTAATTCCTGAAGCTCGTGGCATTTTGACACTGATAGCTGAGCCCAGGGTTGTCTGTCTTTCTCTGTG TGTTTTGCATGATCTTGGATTGGCACCCTACTGTACCCAAACATTAAAAAGCCTGTCTTTCCGTTGAAGA GGACAGGGGTTAAAATGAACGAAGACCCGAAGGTCAATTTAAGCGGGCTGCCTCGGGACTGTATAGATGC CGGTGCTCCAGAGAACATCTCAGCCGCTGTCCCCTCCCAGGGCTCTGTTGCGGAGTCAGAACCCGAGCTC GTTGTCAACCCCTGGGACATTGTCTTGTGCAGCTCAGGAACCCTCATCTGCTGTGAAAATGCCGTTGTGG TCCTTATCATCTTCCACAGCCCCAGCCTGCGAGCCCCCATGTTCCTACTGATAGGCAGCCTGGCTCTTGC AGACCTGCTGGCTGGCCTGGGACTCATCATCAATTTTGTTTTTGCGTACCTGCTTCAGTCAGAAGCCACC AAGCTGGTCACCATCGGACTCATTGTCGCCTCTTTCTCTGCCTCTGTCTGCAGTTTGCTGGCTATTACTG TGGACCGCTACCTCTCGCTATATTACGCCCTGACGTACCACTCCGAGAGGACCGTCACCTTTACCTATGT CATGCTAGTGATGCTCTGGGGAACCTCCATCTGCCTGGGGCTGCTGCCCGTCATGGGCTGGAACTGCTTG AGGGACGAGTCCACCTGCAGCGTGGTCAGACCTCTCACTAAGAACAACGCTGCCATCCTCTCCATCTCCT TCCTCTTCATGTTTGCTCTGATGCTTCAGCTCTACATCCAGATTTGTAAGATTGTGATGAGGCACGCCCA TCAGATAGCCCTGCAGCACCACTTCCTGGCTACATCGCACTATGTGACTACCCGGAAAGGGGTCTCGACC CTGGCTCTCATCCTAGGGACCTTTGCTGCCTGCTGGATGCCTTTCACCCTCTATTCCTTGATCGCCGATT ACACCTACCCTTCGATCTATACCTATGCCACCCTCCTGCCCGCCACCTACAATTCCATCATCAACCCTGT CATTTACGCTTTCAGAAACCAAGAGATCCAGAAAGCCCTCTGCCTCATTTGCTGTGGGTGCATCCCTTCC TCGCTGTCTCAGAGAGCTCGGTCTCCCAGCGATGTGTAGCAGCCTTCTCCTCATAGGACGCTGCCTCTAC CAAGCGCTCCCACCTCCCAGGGCGGCCAGTGATTTCCTTCCTTAAATTCTTTGCACTGGATCTCACAAGC AGAAGCAATGACATCTTTTAGACACGTATTGACAGTGGAAATCATCTTACCAGTGTTTTTTAAAAAAAAA ACAAAACAAAACTCGACTTCTCGGCTCAGCATTCTGTTGTTTGGTTTGGGAGTTAGGATTTGTTTGTTTG TTTGCTTGTTTGTTTGTTTGGAGGGTGTAATGGGACCTCATGTGGCCATGAAATTATACAAAAGTCTCGG GATTTTTTAACCTAGGCTTGAAAATAAATCAAAGTTTTAAAGGAAACTGGAGAAGGAAATACTTTTTCTG AAGGAAATACTTTTTTTTTTTTAATCAAGGTAGATCTTCCATTCTGTATGTATCTAACAGGATAGGAGCT TTGCCATATAACCAAAATAGTTTATATAATTACATTTGGAAGGGCTTGTGTTTATTTCTAGGAATTCAGT AATAAGTGACCAGTAACAGAGGCGCGAACTCCTTTCTTTCCTTTCAGCAGTAGTGACTGCTCTTAAGAAT CACTTTGCAGTTTCTCTGTGTTACAGTTTGGTATGCATGGTTACCTGTGGTAGTCAGATCACTAATTGCA ATATTGCCATGTTAAACCCAGAATTAAAAGAGTCATTTTTTCTTCAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites	AscI-NotI
ACCN	BC055746
Insert Size	1005 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC055746, AAH55746
RefSeq Size	2059 bp
RefSeq ORF	1005 bp
Locus ID	14738
Gene Summary	Receptor with constitutive G(s) signaling activity that stimulates cyclic AMP production (By similarity). Promotes neurite outgrowth and blocks myelin inhibition in neurons.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.