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Eif4a1 (NM_144958) Mouse Untagged Clone
CAT＃: MC205882
Eif4a1 (untagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 1, (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BM-010; Ddx2a; Eif4 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC049915
GGATCATGTCTGCGAGTCAGGATTCTCGATCCAGAGACAATGGCCCCGACGGGATGGAGCCGGAAGGCGT CATCGAGAGTAACTGGAACGAGATTGTGGATAGCTTTGATGACATGAATCTCTCAGAGTCCCTCCTCCGT GGTATTTATGCCTATGGTTTTGAGAAGCCCTCTGCCATCCAGCAGCGAGCTATTCTTCCTTGTATCAAGG GTTATGATGTGATTGCTCAAGCCCAGTCTGGGACTGGGAAAACAGCTACATTTGCCATATCAATTCTGCA GCAGATTGAATTAGATCTAAAGGCCACTCAGGCTTTGGTTCTGGCACCCACACGTGAATTGGCTCAGCAG ATACAAAAGGTGGTTATGGCATTAGGAGACTACATGGGTGCCTCTTGTCATGCCTGCATTGGGGGCACCA ATGTCCGTGCTGAGGTGCAGAAGCTGCAGATGGAAGCTCCCCATATCATCGTGGGTACCCCTGGCCGGGT GTTTGACATGCTTAACCGGAGATACCTGTCCCCCAAATACATCAAGATGTTCGTACTGGATGAAGCAGAT GAAATGTTAAGCCGAGGGTTCAAGGATCAGATCTATGACATATTCCAGAAGCTCAACAGCAACACACAGG TAGTTTTGTTGTCTGCTACAATGCCTTCTGATGTCCTTGAGGTGACCAAGAAATTTATGAGAGACCCTAT TCGGATTCTTGTCAAGAAGGAAGAATTGACCCTGGAGGGTATCCGCCAATTCTACATCAATGTGGAACGA GAGGAGTGGAAGCTTGACACATTGTGTGACTTGTATGAGACGCTGACCATCACCCAGGCAGTCATCTTTA TCAACACCAGAAGGAAGGTGGACTGGCTCACCGAGAAGATGCATGCCCGAGATTTCACTGTTTCTGCCAT GCACGGAGATATGGACCAAAAGGAACGAGATGTGATCATGAGGGAGTTCCGGTCTGGCTCTAGCAGAGTA TTAATTACCACTGACCTGTTGGCCAGAGGCATTGATGTGCAGCAGGTCTCCTTAGTCATCAACTATGACC TTCCCACCAATAGGGAAAACTACATCCACAGAATCGGTCGAGGTGGTCGGTTTGGTCGTAAGGGTGTGGC TATTAACATGGTGACTGAAGAAGACAAGAGGACTCTTCGAGACATTGAGACTTTCTACAACACCTCCATT GAAGAGATGCCCCTCAACGTTGCTGACCTCATTTGAGGGGCTGTCCTGCGACCTGGCCCTAGCCCAGGGT TCAGTCCTGGGGTGGGGCTAAGGAAGAGCTGGAGGGGGGAGGGGAGGGAGCCAAGGGATGGACATCTTGT TTTTGTTTTGGTTTTTTTTTTTTGTTTTGTTTTGTTTCAGTTTTTTTCTCTGAATAAATGTCACTTTTTG AGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_144958 |
| Insert Size | 1221 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC049915, AAH49915 |
| RefSeq Size | 1457 bp |
| RefSeq ORF | 1221 bp |
| Locus ID | 13681 |
| UniProt ID | P60843 |
| Gene Summary | ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206389 | Eif4a1 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1) |
CNY 5,256.00 |
|
| MR206389 | Eif4a1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 1 |
CNY 3,656.00 |
|
| MR206389L3 | Lenti ORF clone of Eif4a1 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 1 |
CNY 4,750.00 |
|
| MR206389L4 | Lenti ORF clone of Eif4a1 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 4A1 (Eif4a1), transcript variant 1 |
CNY 6,056.00 |
