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Rtcb (NM_145422) Mouse Untagged Clone
CAT＃: MC205587
D10Wsu52e (untagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e), (10ug)
CNY 3,768.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI255213; AI463255; D10Wsu52e; FAAP; HSPC117 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC003288
CCCACGCGTCCGGCGTGGAGCGGGTGGACTGCGGGGTGGTTTCTCTTTCTAAGCGTAGCCAGGAGCTATC GCTGGCCTCGCCGCCACCATGAGTCGTAACTACAACGATGAGCTACAGTTCTTGGACAAGATCAATAAAA ACTGCTGGAGGATCAAGAAGGGCTTTGTGCCCAACATGCAGGTTGAAGGAGTGTTTTATGTGAATGATGC TCTGGAAAAACTAATGTTTGAGGAATTAAGGAACGCCTGTCGAGGTGGTGGTGTTGGTGGCTTTCTGCCA GCCATGAAGCAGATTGGCAATGTGGCAGCCCTGCCTGGAATAGTTCATCGGTCTATCGGGCTTCCTGATG TCCATTCAGGCTATGGGTTTGCCATAGGGAACATGGCTGCCTTTGATATGAATGACCCTGAGGCCGTTGT ATCCCCAGGTGGTGTCGGATTTGATATTAACTGTGGTGTCCGCTTGCTAAGAACCAATTTAGATGAGAGC GATGTACAGCCTGTGAAGGAACAACTTGCCCAAGCTATGTTTGACCACATCCCTGTTGGGGTGGGATCAA AAGGTGTCATTCCAATGAATGCCAAAGACTTGGAGGAGGCATTGGAGATGGGGGTGGACTGGTCCCTGAG GGAAGGCTATGCCTGGGCTGAAGACAAGGAGCACTGTGAGGAGTATGGAAGGATGCTGCAAGCCGACCCC AATAAGGTCTCACCCAGGGCAAAGAAAAGGGGCCTTCCTCAGTTGGGGACCCTGGGAGCAGGCAACCATT ATGCAGAAATCCAGGTTGTAGATGAGATTTTCAATGAGTATGCCGCCAAGAAGATGGGCATCGACCATAA GGGACAGGTGTGTGTGATGATCCACAGTGGAAGCAGAGGCTTGGGCCACCAAGTAGCTACAGATGCACTG GTAGCTATGGAAAAGGCCATGAAGAGAGACAAGATTATAGTCAATGACCGGCAGTTGGCTTGTGCTCGGA TTGCATCCCCAGAGGGACAAGACTATCTAAAGGGAATGGCTGCAGCTGGAAACTACGCCTGGGTTAACCG CTCCTCTATGACCTTCTTAACCCGTCAGGCTTTTGCCAAAGTCTTCAACACAACCCCTGATGACCTGGAC CTGCATGTGATCTATGATGTGTCGCACAATATCGCCAAAGTGGAGCAGCACGTGGTGGATGGGAAGGAAC GGACGCTGCTGGTGCACAGGAAGGGATCCACCCGTGCTTTCCCGCCTCACCACCCCCTCATTGCTGTGGA TTATCAACTCACAGGACAACCAGTGCTTATTGGTGGCACCATGGGGACCTGTAGTTACGTTCTGACTGGC ACTGAACAAGGCATGACTGAGACCTTTGGAACAACCTGTCATGGAGCGGGCCGTGCTTTGTCCAGAGCAA AATCACGTCGTAACTTAGATTTCCAAGATGTCTTAGACAAACTGGCAGACATGGGAATTGCAATCCGGGT TGCTTCCCCCAAGCTGGTTATGGAAGAGGCACCAGAGTCCTATAAGAATGTGACAGACGTCGTGAACACC TGCCATGATGCTGGGATCAGCAAGAAGGCCATTAAACTGAGACCAATTGCTGTTATTAAAGGGTAGATCC AAGACTATCAGAGCGTCTGAGGTGCCCATGGACTGAAATTCCTAAGACATGAGACTCAAGGCCTGACAAG ATCCGAATTGCAGAGCTGTTGCTGTGCGTGCCACCATGGCTGGTGGAGAGGCTGCTGTCACAGGGTTGGA AAATAGCCTTCCAGGAGATGTGTCTCTGATGGGAGCAGGGCAAATGAGCCTCTTCTTCCTTTGTCCCACA GGGGTTTGTGACTGTCAATTGGGGTGTGGGAAGGGGCCCAGAAATGCCCTGTTTGGTCATACAAGTCTGT AATCACCAGAGTGGGCTTCTTTATCCCAGGCCCTGTTTGTAGCCCAGAAGGTATCATGGAGAACATGCTA TTAAATAGGTTTTTGAATGTTAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_145422 |
| Insert Size | 1518 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003288, AAH03288 |
| RefSeq Size | 2003 bp |
| RefSeq ORF | 1518 bp |
| Locus ID | 28088 |
| UniProt ID | Q99LF4 |
| Gene Summary | Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs (By similarity). Essential during post-implantation development of embryos.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208125 | D10Wsu52e (tGFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
CNY 4,940.00 |
|
| MR208125 | D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
CNY 3,768.00 |
|
| MR208125L3 | Lenti ORF clone of D10Wsu52e (Myc-DDK-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
CNY 6,370.00 |
|
| MR208125L4 | Lenti ORF clone of D10Wsu52e (mGFP-tagged) - Mouse DNA segment, Chr 10, Wayne State University 52, expressed (D10Wsu52e) |
CNY 6,370.00 |
