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Smug1 (NM_027885) Mouse Untagged Clone
CAT＃: MC205251
Smug1 (untagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1), (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1200013B09Rik; A930006H09Rik; C85220 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC062960
GGAGCTTGTGCAGGGACTTGCAAAGCAAAGGTCCCAGAAAACCCAGTTCGCCACACCGTCCTCAGCTTTC CAGAATGTTCTGGGAGCCCGTGTGGACAAACCAGATTTCTAGCCTGCTATGGTGACACATCCAGGTAATC CCAGCACTCGGGAGACTGAGGCAGGAAGAAGGCCACTGGTTGGAGCCAAAATGCCAGGCAGTTCATTGTT CTGAGAAAAACAGCATGAGAGAGGAAAGCTAGAACCTAGGTCACAGATTCAGGGGTTCCAGTCCCTGGCT GTTTGGCCTCGTGGCTTGTGCTGAGGCAGAACATCATGGTGGAAAGCCACGACCCACCGTGATGAACTGT ACTCTCTGAGAGATGAGATAAATCCTCCCTTCAATTTGCCTTTGCAGGTGACAGCAGCATGGCTGCGTCC CAGACCTTCCCACTGGGGCCTACCCATGAGCCTGCAAGCGCCCTGATGGAGCCTCTGCCTTGTACACGAA GCTTGGCTGAGGGCTTCCTGGAGGAGGAGCTTCGGCTCAATGCCGAGCTGAGCCAGCTGCAGTTCCCGGA GCCCGTGGGTGTCATCTACAACCCGGTGGATTATGCTTGGGAGCCACACCGTAACTATGTGACTCGCTAC TGCCAAGGCCCCAAGGAAGTGCTGTTCTTGGGCATGAACCCAGGACCTTTTGGCATGGCCCAAACAGGGG TACCCTTTGGGGAAGTGAATGTGGTCCGGGACTGGTTGGGCGTTGGGGGCCCTGTGCTGACCCCTCCACA AGAGCACCCCAAGCGACCAGTGTTGGGACTGGAGTGCCCACAGTCAGAGGTGAGCGGAGCCCGATTCTGG GGCTTTTTCCGGACCCTCTGCGGACAGCCTCAAGTCTTCTTCCGGCACTGCTTTGTCCACAATCTGTGTC CTCTACTCTTCTTGGCTCCCAGTGGACGAAACCTTACCCCAGCTGAGCTGCCCGCCAAGCAGCGGGAGCA GCTGCTGTCGATCTGCGACGCAGCCCTCTGCCGGCAGGTGCAGCTGCTAGGGGTGCGTCTGGTAGTGGGA GTGGGGCGGCTGGCAGAGCAGCGAGCCCGAAGAGCTCTAGCAGGGCTGACCCCGGAGGTGCAGGTGGAGG GGCTCCTGCATCCATCTCCTCGAAGCGCACAGGCCAACAAAGGCTGGGAGGCGGCAGCCAGGGAAAGACT CCAGGAGTTGGGGCTGCTGCCTCTGCTAACGGATGAGGGTTCAGCCAGACCTACATAGTAGAGACCCCAG CCAGTGTTGCATTCAGCCTGCTGGACTGTCCTCTAATGGCCCAGGGTGGGCAGCTCTCCTGCTGTCTTCC CCTTCCCTTGAGCCTCTCACCCTTTTGTCTCCAGTGTCTGGTCATGCACGGCATCAGCAGAGGGTTGGGC CTGTGCACTCCACCTACCACATCCTTGCCTTGGGAGTCTCTGTGTGCAGTGCAGAGTCCTAGGTCGGGAT GTCTGAAGTCATATTCGCAGTTTTGGAGGAAAGATGGTTTTTGTTTTAATCTTTCCTGTTTTGCTTTTTA TTGGCATCATGTTTGGCATCGTGTCATGTACTCTAGAAATGTTCTCTAATGCTGATCTACCTCAGCCTTT TTTGACACTAACTACATACTAGTGGGGTTCAGTGTGAGGTTTCCACACAGGCATTCAGCGTACACCGATG GAGTCCAACCCCCTTTTATCTGTCCTCTCCACACTTCCTCTTCCCCAGTCAGTGGACAATCTTTCCAGGA TCCCTGCTCATCCTTACAGATCATCTGGCAGACTCCTGTAATCCCATAACTCAGAGGCAGGAAGATCAGG AGTCCGAGGCCGCCTTCAGCTACATATTGAGTTCAAGGCCAGCTTGGAACACATTAGTCTCTGTCTCAAA AATAAGTAAGTAAATAAGTAATAAAAAAGTGAACTTCTTCAGGGAGCCCAGAGCTGTCTGAGAAGCTGTA TCTGAGGGAGACTTCTGTGTGTCTAGACTAGGAAGGATCTAGATTTTCATCTCTCCCTTTTTTTTGCTCC CTATAAGGCCTTTGAACTTAAAAAAAAATGTATCTATTTATTTTGGTTTTTCGAGGCAGGGTTTCTCTGT GTTGCCCTTACTGTCCTAGAACTCACTTTGTAGACCGGGTTGGCCTCAAACTCAGAGAACTTATATTTTT AAAAAAAATCCCTCTGTTTTGTTTCTGTTCACGTTGTCTATCTGTTTTTAAATTTCGAAACAAGGTCTCA TTGTGTTGGCCTGTAACTCATTATTATAGACCAGCTCCGCTTTGACCTCACAAGAAATCTGCCTACCTCT GCCTCCTGAGTGCTGGGATTAAAAGGATGTGCCACCCTGCCTGACCTGCTTATGAACAACCTCTCTAAGC ACTAAGATCCTTTAAAAAAAAGAGGGTGTGCTGCCTCCTAGCACTGGTGTGAGGTGTGAGGATCGACTGA GATAACACTGCAGAGTGCTTTGCAAAAGGCTGACATATGGTCCTCACTACACACTCTTTCTGCTCACCTC CCTTCGAGGTGGGGTTATATCAATGCACTACAAGGGAATTTCCCGTCTGCTCTGAAGACCCTTGCCTATA ACCCTGCTCGCTTGCTGGAGAATGACCAATGTTACGAGAAACTTAGCCTCTGACTTTTCAGACTAAGCAT TAAAGACTCCTAAGCCTTGTGTTGGGTCTCTCCTCTCTTCCTACACTTCATTGGTGGGAGAATGATTTAG GTTATCTCCTCCCTTTTCTTACCTATTCACTGTCTGAAAAAACTTTTCTCAGGTAGCTTGAGGAGGGGGG ACAGAGACCTCTGATTGCCCTAGCCAAGCTGCAGGGGTTTTGGTAGGGGCCTGCCCTGAAGTTCATCAGG TACTAGGGCAACTGTTGGGAACAATGAGGAGCTGGTGTCTCATCTGTCTCTTTAATGGTTGGTTGGATGA ATCTGTGGTAAGGGCTGGGAAGTGAGCCACAGTGAGAGCAGCAGGTCTTAGGGCCATCTTAATGAAGATA CATGGTCTCTCTATCTCCTGGGATACAAGTCATCTCCAGCATTCTAAAAATTAGTATATTTCTATCTCAG CTCCAGAGAGGGAGACTAAACCTTTGAGGATTCCCCAGTGATAGAAGTGTTGTAATTATTCATGTTGGGG GTCTCAGAGCATTCCAGAATTTGTTGAACTTTGACTCAGGATGGAGGCCAACCCTCCAGAAAGACCAGCC GTATGTATTGAGGGTTGGGGCTTAGCCTGAATGCCAGAATGGGACAAGGATAGAGATTAAGTTCAGCCAC ATGGCCCATGATCTCGACCAGTTATACCTCCACAGTGACCCTAGCCAGCTCTCTTGGACACTTGAGTTTC CACTAAGCTTGCTGGCTGGTGCACACATGTATATCTGTGTTTCTTTTTGGGTATTTGGTGCTGGTTTGTG TATTCATTCATTCATTCATTCATTCATGTGCTCTGTGTGTCATTTTGGAGTTCTGGGCATCAAGCCCAGA ATTTTGTGAATGGGAAGCATGCACTCTTCTATCACTGAGCTACATCCCTTGATTTGTATTTGTGATAAAT TGTAATAAATAAATAAACAAACACAGTGTTTTCCCCCCTGAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_027885 |
| Insert Size | 840 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC062960, AAH62960 |
| RefSeq Size | 3635 bp |
| RefSeq ORF | 840 bp |
| Locus ID | 71726 |
| UniProt ID | Q6P5C5 |
| Gene Summary | Recognizes base lesions in the genome and initiates base excision DNA repair. Acts as a monofunctional DNA glycosylase specific for uracil (U) residues in DNA with a preference for single-stranded DNA substrates. The activity is greater toward mismatches (U/G) compared to matches (U/A). Excises uracil (U), 5-formyluracil (fU) and uracil derivatives bearing an oxidized group at C5 [5-hydroxyuracil (hoU) and 5-hydroxymethyluracil (hmU)] in ssDNA and dsDNA, but not analogous cytosine derivatives (5-hydroxycytosine and 5-formylcytosine), nor other oxidized bases. The activity is damage-specific and salt-dependent. The substrate preference is the following: ssDNA > dsDNA (G pair) = dsDNA (A pair) at low salt concentration, and dsDNA (G pair) > dsDNA (A pair) > ssDNA at high salt concentration.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG203761 | Smug1 (tGFP-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 2,850.00 |
|
| MR203761 | Smug1 (Myc-DDK-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 2,400.00 |
|
| MR203761L3 | Lenti ORF clone of Smug1 (Myc-DDK-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 4,750.00 |
|
| MR203761L4 | Lenti ORF clone of Smug1 (mGFP-tagged) - Mouse single-strand selective monofunctional uracil DNA glycosylase (Smug1) |
CNY 4,750.00 |
