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Cyld (NM_173369) Mouse Untagged Clone
CAT＃: MC205105
Cyld (untagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 2, (10ug)
CN¥ 6,976.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2010013M14Rik; 2900009M21Rik; C130039D01Rik; CDMT; CYLD1; EAC; mKIAA0849 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC042438
CTGCGCGGGGGCCCGCGCGCCTGAGGCACTTTGAATTGCTGTCTTTTTACAACATGGATGCCAGGTTGCT AAAATCTTGCTTTGGGACTTACAGCGAGTTCATTTTTTTGGATTTTATGACAGATTTACTAGTGGCTCCT TTTTGCCAGTCAATGTTCTGAAAGTTACTGTCACAATGAGTTCAGGCCTGTGGAGCCAAGAGAAAGTTAC TTCACCCTACTGGGAAGAACGGATTTTTTATCTGCTTCTTCAAGAATGCAGTGTAACAGACAAACAAACT CAGAAGCTGCTGAAAGTACCCAAAGGGAGTATAGGACAGTACATCCAAGACCGTTCTGTGGGGCATTCAA GAGTTCCTTCCACAAAAGGCAAGAAAAATCAGATTGGATTAAAAATCTTGGAGCAACCGCATGCAGTTCT GTTTGTTGATGAAAAGGATGTTGTAGAAATAAATGAAAAATTCACAGAGTTACTGTTGGCAATTACCAAC TGTGAGGAGAGGCTCAGCCTATTTAGAAACAGACTCCGACTAAGTAAAGGCCTCCAGGTAGACGTGGGCA GTCCTGTGAAAGTACAGCTGCGATCTGGGGAAGAGAAATTTCCAGGAGTTGTACGCTTCAGAGGACCTTT ATTAGCGGAGAGGACGGTGTCGGGGATTTTCTTTGGAGTAGAATTATTGGAAGAAGGTCGTGGTCAAGGT TTCACGGATGGGGTATATCAAGGGAAGCAGCTTTTCCAGTGTGATGAGGACTGTGGCGTGTTTGTTGCAT TGGACAAACTAGAACTTATAGAAGATGATGACAATGGATTGGAAAGTGATTTTGCAGGCCCAGGAGATAC AATGCAGGTTGAACCTCCCCCTTTGGAAATAAACTCCAGAGTTTCTTTGAAGGTTGGAGAAAGTACAGAA TCTGGAACAGTAATATTCTGTGATGTTTTACCAGGAAAAGAGAGTCTAGGATATTTTGTTGGTGTGGACA TGGATAACCCTATTGGCAACTGGGATGGAAGGTTTGATGGAGTACAGCTCTGTAGTTTTGCAAGTGTTGA AAGTACAATTCTCCTGCACATCAATGACATCATCCCAGATAGCGTGACACAGGAAAGGAGGCCTCCCAAA CTTGCCTTTATGTCAAGAGGTGTAGGTGACAAAGGTTCATCTAGTCATAATAAACCAAAGGTTACAGGAT CTACCTCAGACCCTGGAAGTAGAAACAGATCTGAATTATTTTATACCTTAAATGGGTCATCTGTTGACTC ACAACAATCCAAGTCCAAAAATCCATGGTACATTGATGAAGTTGCAGAAGACCCTGCAAAGTCACTTACA GAGATGTCTTCGGACTTCGGACATTCATCTCCTCCACCGCAGCCTCCTTCCATGAACTCCTTGTCTAGCG AGAACAGATTCCACTCCTTACCCTTCAGCCTGACAAAGATGCCCAATACTAATGGCAGCATGGCTCATAG TCCACTCTCTCTGTCAGTGCAGTCTGTGATGGGGGAGCTGAACAGCACACCTGTCCAGGAGAGTCCACCC TTGCCCATCTCTTCTGGGAATGCACACGGGCTAGAGGTGGGCTCACTGGCTGAAGTAAAAGAGAACCCCC CGTTCTATGGGGTTATCCGTTGGATTGGCCAGCCACCAGGGCTCAGTGACGTGCTAGCTGGACTGGAACT GGAAGATGAATGCGCAGGCTGTACAGATGGAACTTTCAGGGGCACGCGGTATTTCACGTGTGCCCTGAAG AAGGCACTGTTTGTGAAACTGAAGAGCTGCAGACCGGACTCTAGGTTTGCATCCTTGCAGCCTGTTTCCA ATCAAATTGAAAGGTGTAACTCTTTAGCATTTGGGGGCTATTTAAGTGAAGTAGTAGAAGAAAATACTCC ACCTAAAATGGAAAAGGAAGGTTTAGAGATAATGATTGGAAAGAAGAAAGGCATCCAGGGCCATTACAAT TCTTGTTACTTAGACTCAACTTTATTCTGCTTATTTGCTTTTAGTTCTGCCCTGGACACTGTGTTACTTA GACCCAAAGAGAAGAATGATATAGAGTATTACAGTGAGACTCAGGAGCTACTGAGGACAGAGATAGTCAA TCCTCTGAGAATATATGGATATGTGTGTGCCACAAAGATTATGAAACTGAGGAAAATACTTGAAAAAGTT GAGGCTGCATCAGGTTTTACCTCTGAAGAAAAAGATCCTGAAGAATTCTTAAATATCCTGTTTCATGATA TTTTAAGGGTTGAACCATTGTTAAAAATAAGATCAGCAGGTCAAAAAGTTCAAGACTGTAACTTCTATCA AATTTTTATGGAAAAAAATGAGAAAGTTGGAGTACCCACAATTCAGCAGTTATTAGAATGGTCTTTTATC AACAGCAACCTGAAATTTGCAGAGGCACCATCATGCTTGATTATCCAGATGCCTCGGTTTGGAAAAGACT TTAAACTATTTAAAAAAATTTTTCCTTCCCTGGAATTAAATATAACAGATTTACTTGAAGACACTCCCAG GCAGTGCCGCATCTGTGGAGGACTTGCGATGTACGAGTGCAGGGAGTGCTATGACGATCCGGACATCTCA GCTGGGAAGATCAAGCAGTTCTGTAAGACCTGCAGCACTCAGGTTCACCTTCATCCCAGGAGGTTGAATC ATTCTTATCATCCAGTATCACTTCCCAAAGACTTGCCTGACTGGGACTGGAGACACGGCTGCATCCCCTG TCAGAAGATGGAGTTATTTGCTGTTCTCTGCATAGAAACGAGCCACTATGTTGCTTTTGTGAAGTATGGG AAGGATGACTCTGCCTGGCTTTTCTTTGACAGCATGGCGGATCGAGATGGTGGTCAGAATGGCTTCAACA TTCCACAAGTGACGCCCTGCCCAGAAGTGGGAGAGTACTTGAAGATGTCTCTGGAGGACCTGCACTCTTT GGATTCCAGAAGGATTCAAGGCTGTGCGCGCAGACTTCTTTGCGATGCATACATGTGCATGTACCAGAGT CCAACCATGAGCCTGTACAAATAACCAGGTTATCCGCAGTGGGCAGAGACAGCACAAACCTTAGTGCTGT GTCTTCCTCCAGCTTGTGGTTCTGTCCGGCACCCATTGCCGGCAATGGGTGTCTCTTTTGATGATGGCCC TTCAGCAAAGGATTCCTGTGTTTCCAAACAAATTGCTTTTGTGTTCCTGAAGTATTTAATAAGAAGCATT TTGCACTCTAGGAAGTATGTTTGTGTTGATTTTTTAAGAAGTCTAAATGGATTTATTAATATCTGACACT TTAAGTTAACTGCATTGATGATACGATATTTTTGGAAGCATACAATTTTAATTGTGACGTCTAAAACCTC TTTTAGTCCATTGAGGATGTAAATAAATATTTCTTCTTATGGACCAGGGATATGAAATCATTTTCCTTTT GTAGCTAATGATTGCCTTGAGAAAGACATAATATGATTTTATTAAGAGTCTGCCTTTACTCCAATTGTTG AAGGTGTGCTGAGTTTAATTGTTAATTTTCCTATATAGTGCTGATTTCCTGCATGTCTGTACTTTGCTGA GTTTCTGTGTTCCTGCAGCAGTGGTCAGAACATGCTGAAATTCCTTTCATGGTGTTATTTTTTATTTGTT CTAGTTTTGAGATAAATGAGTGACTCTGTCATAAAATGTCCATTTTTGATTTGTTCTTGAAGAATTAGGG TACAAAACAAGTATCGTAGTCATAGTAACAACTGTTACTGAACAGGTCTTCTGTAGGGGCTCCTAAGTAT GAGACAACATGCAGTGGTTTCTGAAATGACTGAGCGATAAGTAAATTATATATTCTTTTTTTAAAAGATT TATTTTATTTATATGAGTACACTGTAGCTGTCTTCAGACACACCAGAAGAGGGCATCAGATCTTATTACA GATGGTTGTGAGCCACCATGTGGTTGCTGGGAATTGAACTCAGGACCTTTGGAAGGGCAGTCAGTGCTCT TAACTGCTGAGCTATCTCTCTAGCCCCAAATTATATATTCTTATACAGACCCACAGTCCAGAGAAAGTGA AGCTGGCTCAGAGTTAGCACCAACATTTCCCTGAAAAAATGTGTTAGGTGTTTAAAAATCTCATCGTAAA TAAATTATTTTATTTTTAGTTTAAGTGAATTTCTCTTAATTTAAATAAGACTAATATTTTTGTTTGACTG AAGACCCTTGTACTCTCTGGTGTCAGTTTGGTGTCACTTGAAACAATGACAACCACGTCAGCATGTTACT GCAATGTCAGTGATGGTTTCAAAGGAGAAAATGGATGTCATCTTAGGACTGCTTGTGTGTGTATAGACCG TGGTGGATGCTGTAGAGTGCTTGTTGTCTGAGTGTTGTTTTGTGGTATGGCTGTCTCTCTGAATAATGTC AACCTTACAGAAAGAGAAGGTGTTATATGTGTAACATATGGCTTTATAAAAGATTTAATGTCAAAAAAAA AAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_173369 |
| Insert Size | 2859 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC042438, AAH42438 |
| RefSeq Size | 4501 bp |
| RefSeq ORF | 2859 bp |
| Locus ID | 74256 |
| UniProt ID | Q80TQ2 |
| Gene Summary | This gene encodes a protein that is a member of the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. This protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway. In humans mutations in this gene have been associated with cylindromatosis, an autosomal dominant predisposition to tumors of skin appendages. In mouse deficiency of this gene impairs thymocyte development and increases susceptibility to skin and colon tumors. A pseudogene of this gene has been identified on chromosome 1. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (2) encodes isoform b. Both variants 2 and 4 encode isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG211292 | Cyld (tGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld) |
CN¥ 7,890.00 |
|
| MG227170 | Cyld (tGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld) transcript variant 2, (10ug) |
CN¥ 7,890.00 |
|
| MR227170 | Cyld (Myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 2 |
CN¥ 6,976.00 |
|
| MR227170L3 | Lenti ORF clone of Cyld (Myc-DDK-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 2 |
CN¥ 9,030.00 |
|
| MR227170L4 | Lenti ORF clone of Cyld (mGFP-tagged) - Mouse cylindromatosis (turban tumor syndrome) (Cyld), transcript variant 2 |
CN¥ 9,030.00 |
