Rubcn (NM_172615) Mouse Untagged Clone

Name: Rubcn (NM_172615) Mouse Untagged Clone
SKU: MC204861
Price: 6896.00

CAT＃: MC204861

1700021K19Rik (untagged) - Mouse RIKEN cDNA 1700021K19 gene (1700021K19Rik), transcript variant 2, (10ug)

Datasheet

SDS

"NM_172615" in other vectors (4)

CN¥ 6,896.00

货期*

现货

规格

Product images

经常一起买 (3)

TF81001

TurboFectin Transfection Reagent (1 mL in 1 vial)

CN¥ 4,090.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CN¥ 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CN¥ 480.00

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1700021K19Rik; 5330403K09; Kiaa0226; mKIAA0226; Rubicon
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC067390 GGAAGTGGGGGTGGCAGGGGTAGAGCGGCAGCTGAGGCAGCAGTCCTTGACTCTGTCGCTGTCAGGGTGT AGTGCATGGTTCTAAGCTCACCAGCCATGGTGCCTCCCCTCTCTTGAGGGTGCTCCGGGTACCCAGGCGG TCGAAGCGGCCTGGAGCCTCTCTCTGGCCCCGAGACGGCCTTATAGTCCGGATGCGTCCGGAGGGCGCGG GAATGGATCTTGGCGGCGGCGATGGGGAGCGTCTGCTAGAGAAGAGCAGGAGGGAGCACTGGCAGCTGCT GGGTAATTTGAAGACGACTGTGGAAGGTTTGGTGTCAGCCAACTGCCCCAATGTCTGGTCCAAGTATGGT GGCCTGGAGCGGCTGTGCAGGGACATGCAGAACATCCTGTATCATGGGCTCATCCATGACCAGGTGTGTT GCCGCCAGGCTGATTACTGGCAGTTTGTGAAAGGCATTCGGTGGCTCAGCCCGCACTCAGCCCTTCACGT GGAGAAGTTCATCAGTTTGCATGAGAGCGACCAGAGCGACACTGACAGCGTGAGTGAGCGTGCTGTTGCA GAGCTGTGGCTGCAGCATAGCCTGCAGTGCCACTGCCTCTCAGCCCAGCTCCGGCCTCTGCTCGGGGACA GACAGTACATCAGAAAATTCTACACAGAAACTGCCTTCCTGCTGAGTGACGCCCACGTCACAGCCATGCT CCAGTGCCTGGAAGCAGTGGAACAGAACAACCCCCGTCTTCTGGCTCAGATCGATGCATCCATGTTTGCC AGAAAGCAGGAGAGCCCGCTGCTGGTCACAAAGAGCCAGAGTCTGACCGCTCTGCCTGGTTCCACATACA CCCCTCCAGCTAGCTATGCTCAGCATTCCTACTTTGGGTCCTCCTCCAGCCTTCAGTCTATGCCACAGTC CAGCCACAGCTCAGAGAGAAGATCTACTTCCTTTTCACTCTCTGGCCCTTCCTGGCAACCTCAAGAAGAC AGAGAATGCCTCTCACCAGCAGAGACTCAAACCACCCCAGCTCCGCTGCCTTCAGACTCTACTCTAGCCC AGGATTCCCCACTGACAGCACAGGAGATGAGCGACAGCACTCTGACTAGCTCCCTAGAGGCATCCTGGGT CAGCAGCCAGAATGACTCCCCAAGTGATGTCAGTGAGGGGCCCGAGTACCTGGCCATTGGCAACCCAGCC CCTCATGGCCGGACTGCCAGTTGTGAGAGTCACAGCAGCAACGGCGAGAGCAGCAGCTCTCACTTGTTCT CCTCCAGCAGCTCTCAAAAGCTGGAGTCTGCTGCCTCTTCTCTAGGGGACCAAGAGGAAGGCAGGCTGAG TCAGGCAGGCAGCGTCCTTCGCAGGTCCAGCTTCTCAGAAGGGCAGACAGCCCCTGTCGCCAGCGGGACA AAGAAGAGCCATATTCGCTCCCACTCGGACACCAACATTGCCTCCAGAGGAGCCGCAGAGGGTGGTCAGT ACCTCTGCTCAGGAGAAGGCATGTTTAGAAGACCATCAGAAGGACAGTCCCTCATCAGTTACCTCTCTGA GCAAGACTTTGGCAGCTGTGCAGACCTGGAAAAGGAAAACGCCCACTTCAGCATCTCCGAGTCCTTGATT GCCGCCATTGAGCTGATGAAGTGCAACATGATGAGCCAGTGTCTAGAAGAGGAGGAAGTAGAGGAGGAAG ATAGTGACCGAGAGATCCAGGAACTGAAGCAGAAGATCCGCCTTCGGCGCCAGCAGATCCGCACCAAAAA CCTGCTCCCTGCGTACCGGGAGACTGAGAATGGAAGCTTCCGGGTCACCTCCAGCAGCTCCCAGTTCAGT TCACGGGATTCGACGCAGCTCTCTGAGTCCGGCTCTGCTGAGGATGCTGACGACTTGGAAATCCAAGACG CTGACATCCGGAGGAGTGCAGTCTCAAACGGCAAATCATCCTTCTCCCAGAATCTCTCGCACTGCTTCCT GCACTCCACATCGGCTGAGGCAGTGGCCATGGGGCTTCTGAAGCAGTTTGAGGGGATGCAGCTTCCAACT GCCTCGGAGCTCGAGTGGCTAGTCCCAGAGCATGATGCTCCACAGAAGCTCCTACCCATCCCTGACTCCC TGCCCATCTCACCAGATGACGGGCAGCACGCGGACATCTACAAGCTGCGAATCCGCGTCCGTGGCAACCT GGAGTGGGCTCCGCCCCGGCCGCAGATCATTTTTAATGTTCATCCAGCCCCCACGAGGAAGATTGCCGTG GCCAAGCAGAATTACCGCTGTGCGGGATGTGGCATCCGGACGGACCCCGACTATATCAAGCGGCTGCGGT ACTGTGAGTACCTAGGCAAGTACTTCTGCCAGTGCTGCCACGAGAACGCCCAGATGGTCGTCCCCAGCCG CATTCTGCGCAAATGGGACTTCAGCAAGTACTATGTCAGCAACTTCTCCAAGGACCTGCTCCTGAAGATC TGGAATGATCCTCTCTTCAATGTGCAGGACATCAATAGCGCACTCTACAGGAAGGTCAAGCTGCTTAACC AAGTCCGGCTGCTGCGGGTTCAGCTGTACCACATGAAGAACATGTTTAAGACATGCCGACTGGCCAAAGA GCTCCTGGATTCCTTTGACGTGGTTCCAGGCCACCTGACAGAGGACCTCCATCTGTACTCGCTGAGTGAC CTGACTGCAACCAAGAAGGGAGAACTGGGGCCCCGGCTGGCGGAGCTCACTAGAGCGGGAGCCGCCCACG TGGAGAGACGCATGCTGTGTCAGGCCAAGGGCTTCATCTGTGAATTCTGTCAGAATGAGGAGGATGTCAT CTTTCCTTTTGAGCTGCATAAGTGCCGGACTTGTGAAGAGTGTAAAGCGTGTTACCATAAAACTTGCTTC AAGTCTGGACGCTGCCCCCGGTGTGAGCGGCTGCAGGCCCGACGGGAGCTGCTGGCCAAGCAGAGCCTGG AGTCCTACCTGTCTGACTATGAGGAGGAGCCCACAGAAGCCTTGGCTCTAGAGGCCACCGTCCTAGAGAC CACCTGAGGAATGCACTAAGCCCTTTGTGTAAGGGTCTGGCGAGGGTCAGATATAGACTGAGCCACACCT TCAAGGAAGGTGGGGCCTTTTAAATATATATATATTGTCATTTTTTATGACTTGTCTGCTGTCCAAGTGT AGGCAACCTTTCTTGGTTGATGGATCCAGTCTCTTGTGACAGCTGTCTGTGGGCACTGGGTATTTTTGTC AGGGGAATGGACATCGAGACCCAGTTCTGACCCGCTCGGCCTCTTCTACTAGGACCAGATCTGGTTACAG CTGCAGCTAACGATCATACCCACTGTTGACTTAGTTCCTCAGAGTCTGAGCCAGGCCTCTCTCAGCCAGC GACCTTCTCCTTTGCTGCTGAAGCAGGAGAATGGTCTTTTCTTAGGCCTAGTCCTAACAGAGACTAAGAC TGTGCCCTGAGTCACGGGAGAGAAGGGGGGTATACAACGGGCATTCTGTCTGGCCACTTAGGTATACGGG GGGGCAGGGCAGGTAGGAACAGAACCATCCTTGTTCTAGCATCTTCGGGATCTCAAAGGGCAACCATGAC TTCCCTGTTTCAAATATTCCTGGCATAGGTGGGCACAAGCTGGTTTGTGTACTCTGGTGGGCTTGGATCA AAGACCAGATCCGAGTCTTAGGTCAGGCCACAAGCCTATAAGTGCGGCTACAGCTGGTGCTTAAGGGGCG GCTTTTTCAGATAGGCATCTCCTCCGGGGTGGTCAGTCTCTCAGTTAACTATCGTGAGTGCAGGTGTGCT TCATGCTCAGGCAAGTCCCTGATGCTACCTAAGGCGAGGATAGAAACCCCTCCCACCATCTGAATAGTGG GTTCCTTACTGCTCTGTACGACTATGTGGTCATTGTGGTTCCAGTACTGATATGACCCAACTCCATTCTC TGTCCCCTTCCTCCAGAGAGCTGCAGCCCTTCCTCTTGGCTGATCTCTTGCCTTAGTTTCTTTTCTCAGA GTTGGGGGAAGCCAGCCAACTAGTAGCACTAAGTAAAGCACTTGGCAGTTTGTGAATGTGTCTGTTTGCT CAGAGTAACAGGACCTTTATGCAGTTAACTAACTCTTCAGCTTTGTAATCTATGTCAAACCATCCATTTC ATGGTACTAATTTTAATAGACTCTTCCCCTATCACCTAATGTGCATTTTTTGTGGGAATTGCCCTGGACT AGGCTGCACATGATGGTGGTGGTGTTTGAGTTGCTGCAGCACCGACCTCGGTAAACACTGGTCAGGGCTG CCTCTCAGAGCCTGGCAGTTCTGTGAACTTAATCCACAGCCCTCTTGTTCTGGAGCTGGCCTGTGGCTTC CTGTACTTGACCGCTAGTAAAATCATTGGTCCAGGCAGTGTCGCAAGCCGTCAAATGGAGTTGCATGTTG TTGATGGAAGCTGACAGGTAGCTGTGCTTAGAGGGAACGAGGGATCTCCCAGAGAGGCAGAAGGGGCATG ACATAACAGCCAAGCTGAGCTCCAGCAGAGCAAGAGGGAGCATGCCAGGCTAGTTCCAAGGTGTGGCTCC TCTGCCTCACACTTGGGATCTGACAACTACATGGACTGGGACTGCTCTCAGTAGTCGATGAGGCAGAAAG CCGAGGCCAGAGCCAGTTCTGCAGAGCTGGTTAGAAATCCCTCCCAATTCACTTATCACTGAGTCAGACC TCAGAGACAGACTCTGACTCCAGGTCCAGTTCCTGGCTGACTTCCGTCCAGCTGCTCAGTGAAGTCACCA CACTGGAGGAGACTCCACCCATGCTCCCAGTCTACAAAGGCTCAAGCAGCCTCACTGACGTCCCTGTTCC TTTCCACCTCCCCACCCCACCCCATCCCGCTAGGTCACATACCTTCTAGAGCTCCTGAGTCCGCTGTCAT TACTCACCAAATACCCATGTCTGTTTATTTGAGGAGCCGGAGCAGGGGGTGTGGTGAAAGCACTTTGTCA CTTACAGTATCTGAGGATCCCCTGCTACACTGATGGGAGGTGGGGATGGAGCTTAGGGAGGGGAGCAGCA CTCTGTACAGAGGGGACAGCTCCAAGTGCTCTGCACTTAAGGGGCCCTTCGTCCTCTGCTGAGGGCTGGA GAGCTTTTGCCGAAGAGAAAAGGAGCAAGAGTCTAAATGAAAACGCTGTTTGTGTACAACCTGTGTTTAG AACTGGATTCTCACCTGCTGTAATTTACGCACATCCTGACAGTCCATGGTCATCTTTAATAAACTAAGGA AAATAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	NM_172615
Insert Size	2826 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC067390, AAH67390
RefSeq Size	5267 bp
RefSeq ORF	2826 bp
Locus ID	100502698
UniProt ID	Q80U62
Gene Summary	Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process (PubMed:19270693). Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform b), compared to isoform a.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MG211261	1700021K19Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700021K19 gene (1700021K19Rik)	10 ug 产品选项验证失败所有选项都是必需的	CN¥ 11,944.00
MR211261	1700021K19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700021K19 gene (1700021K19Rik), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CN¥ 10,344.00
MR211261L3	Lenti ORF clone of 1700021K19Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700021K19 gene (1700021K19Rik), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CN¥ 9,030.00
MR211261L4	Lenti ORF clone of 1700021K19Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700021K19 gene (1700021K19Rik), transcript variant 2	10 ug 产品选项验证失败所有选项都是必需的	CN¥ 9,030.00

其它Rubcn产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies