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Cbfb (NM_022309) Mouse Untagged Clone
CAT＃: MC204426
Cbfb (untagged) - Mouse core binding factor beta (Cbfb), transcript variant 1, (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI893578; PEA2; Pebp2; PEBP2b; Pebpb2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC026749
CGGACGCGTGGGCGCGGGTGGGCGGTCAAGGCGGTCAGCACCGGAGCCCGCCAGCGGGTGCCCGCGCGAG CCTGGGGAGCGCGGCCGGCCGGCGCGGCCTGAGGGCGGGAAGATGCCGCGCGTCGTCCCGGACCAGAGGA GCAAGTTCGAGAACGAGGAGTTCTTCAGGAAGCTGAGCCGCGAGTGCGAGATTAAGTACACGGGCTTCAG GGACCGGCCCCACGAGGAGCGCCAGACACGCTTCCAGAACGCCTGCCGCGACGGTCGCTCGGAGATCGCT TTTGTGGCTACAGGAACCAATCTGTCTCTCCAGTTTTTTCCGGCCAGCTGGCAGGGAGAACAGCGACAAA CACCTAGCCGGGAATATGTCGACTTAGAGAGAGAAGCAGGCAAGGTATACTTGAAGGCTCCCATGATTCT GAATGGAGTGTGTGTTATATGGAAGGGCTGGATTGATCTCCACAGATTGGATGGTATGGGTTGCCTGGAG TTTGATGAGGAGCGAGCCCAGCAGGAAGATGCATTAGCACAACAGGCCTTTGAAGAGGCTCGAAGAAGAA CTCGAGAATTTGAGGATAGAGACAGGTCTCACCGGGAGGAAATGGAGGCAAGAAGACAGCAAGACCCTAG TCCTGGTTCTAACTTAGGTGGCGGTGATGATCTCAAACTTCGTTAAGTGGAGCACAGCTTATGTGCCCCA TCTCTACACACACTGCTTCTAGTTGGTATAAATAACTCATTGGGCGACCAGAAACTGTGACAACTGGAGG GACTACAGTTGGTTTCATGGCCTGAGGCAGTGAAGACGTCACCCACTGCCACGGTTTTGCACTATAATGC CTGCATTTCTGATTTTTAAAATATGTAGCCAGTAATAATTTGAAATTTTTTTCTATGCAAGCTTATCTTG TTGGCATTATTTTAGTTGTATCGAAAGTATATACTTTCTTCATTTTAACTTAAAAGCTCATGTCATTTAG AAACAAGATAAGAAATTAAAATTTGTATCAGGGTTGCTTAACATTCTTTTATACTTTCAGACGAGTTGGT TATACAGCTTTCCTCCTTAGATAAGGGTTCTTGGTTTTTGTTTGTTTTCTCTACATCATTTCGTGTTTTT GTATTCTGCACCATTTTACAAATGAAGATGTGTTTGCGGTTATTTTTTCCAGCTATAAACCTAGAATAGA GCTGTCTGCCACAGCCTCCTAAAACAAACGTTTACAGTTGTTAAAGCCACAGTATCCTTTCAAATGCTGA TAGTCACCCTATCCCCTTTAGACAAAAATTCACATTAGTATTAAATTTTGTCTCAGACCCTGTTTCAAGG TATATCTTGTAATCATGAGTTTGGGTAGAGATGCTCTTCACAGGTGAGAATGCTCTACAGAAATGCCCAA AGAAGCCACAGACTGGCTTCTGTTTTATTCAGGGATTTTTTTTTTAATCAGTCAGAAAAAGGATACTGGA ACTTGTTGTATGTAACAGCATATTAAACTGGAGGCAGTGATAAATCAGCTACACAGGTAAATTGTGTTAA AATAATGTTTAAGATAGCTGCTTTCATGTGTATTTTGTATTTTGTATTGCATGCTTTTGTAGAAACAAAA TTGGGTGATGAAGATGAGTCTTAGAAAATGTTCATCTGGGCAGAAGATGCATTTCTCCCATTGATTCTGG GCAAAACATTCACAGATATAGATGATGGTATTTTACAAAAGGACTATTAATGGTGCCTTTTGAGATGAAT CAATGTAACGATATTTTTAAAACAGGCTTAGTGTCAAATTGCAATTTTTTAAATATAAAACTGGAAAAAG TGCTAAGTCATTGGCACATGCTTACAATACTTTCCGTGGTCGTATTCATTAAATGTTACTACATTTCTGA ATTTTTGCAAAAATGTATTTTATCATAAAATGGCATTATTTTAGAAAATTTTAAAAACTGACATGGTCAA TTCAGAAAAGTCTGAATAAGGTCATTGCATTTAAAAAGCATATAAAGATACTTGATTGTGGAAGTGTGTC TTTCATTGTATATAGGTCTTATAATTCATAAACAAGTACCCTGTATCTCAAAATAAAAATATTTGTTATA TATTTGAAGTCGTGCATGATAAGGATTGTGTTTGAATTATTATAAACAATAATGTGTTACAGAAGGTGAT GCTGACCTTGTGTTACTGAGCACTATGAATGAATTTGCTTGGTATTTGGTGTTGTACAGCTCACATGTTT ACACTCTCAGTGCCCTGATGCCTCTCGGGAGTTACCTTTTTAAAGTGATCCTTTCTTTTTTAAAATAACA TCTCGGTGGTACATTTTCTCTGTTGCTTCTCGCCAAGAGCTTCCAGTCCGGCAGTCAGTAAGATCACATG GTGTTAATGTGGCCGTCCCTCTGTAAATAGCAAATGTTCCTTACTGGTTCTGCACTGCTTCTGTCTGTCT TAATGCTGGCGTTAAGATCATAAACTCCTTTTCTTCGGTAGTGCAGGCTTTGAAAATTAAGTTATTGATG CAATTTCATATTTTTCATAATCTGTATTTAAAATGACATCATTGCATCATTTTTTAAAGATTCATCTCTA TTAAAACTTGCCTTAAGCTTCCAGATTGCTTTGCCGTTAGCTTTACTGTATATTTGTATGTTTTATTTAC TTTCACATTAAAATTCTGTGTACTGAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_022309 |
| Insert Size | 564 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026749, AAH26749 |
| RefSeq Size | 2705 bp |
| RefSeq ORF | 564 bp |
| Locus ID | 12400 |
| UniProt ID | Q08024 |
| Gene Summary | Forms the heterodimeric complex core-binding factor (CBF) with RUNX family proteins (RUNX1, RUNX2, and RUNX3). RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation (PubMed:18258917).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG201754 | Cbfb (tGFP-tagged) - Mouse core binding factor beta (Cbfb) |
CNY 2,850.00 |
|
| MR201754 | Cbfb (Myc-DDK-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1 |
CNY 2,400.00 |
|
| MR201754L3 | Lenti ORF clone of Cbfb (Myc-DDK-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1 |
CNY 4,750.00 |
|
| MR201754L4 | Lenti ORF clone of Cbfb (mGFP-tagged) - Mouse core binding factor beta (Cbfb), transcript variant 1 |
CNY 4,750.00 |
