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Akr1b3 (NM_009658) Mouse Untagged Clone
CAT＃: MC203763
Akr1b3 (untagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3), (10ug)
CNY 2,400.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Ahr-1; Ahr1; Akr1b1; Aldor1; Aldr1; ALR2; AR |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC021655
CCACGCGTCCGTTTAAAGGTGCGCGCCCGAGCCAGCGCTGCACATTTGCCGGTCCGGGTTTGTAACGTGC AGCGATCATGGCCAGCCATCTGGAACTCAACAACGGCACCAAGATGCCCACCCTGGGCCTGGGCACCTGG AAGTCCCCTCCTGGCCAGGTGACTGAGGCCGTGAAAGTTGCTATTGACTTGGGGTACCGCCACATTGACT GCGCCCAGGTGTACCAGAATGAGAAGGAGGTGGGAGTGGCCCTCCAGGAGAAGCTCAAGGAGCAGGTGGT GAAGCGGCAGGATCTCTTCATTGTCAGCAAGCTGTGGTGCACGTTCCATGACAAGAGCATGGTGAAAGGA GCCTTCCAGAAGACACTGAGCGACCTGCAGCTGGACTACCTGGATCTCTACCTTATTCACTGGCCAACGG GGTTCAAGCCTGGGCCCGACTATTTCCCACTGGATGCCTCAGGGAACGTGATACCTAGTGACACCGATTT TGTGGACACTTGGACGGCTATGGAACAACTAGTGGATGAAGGTTTGGTGAAAACAATCGGTGTCTCCAAC TTCAACCCTCTTCAGATTGAGAGGATCTTGAACAAACCTGGCTTAAAATATAAGCCTGCGGTGAACCAGA TCGAGTGCCACCCGTACCTAACTCAGGAGAAGCTGATTGAATACTGCCACAGCAAAGGCATCGTGGTGAC AGCATATAGTCCCCTTGGTTCTCCTGACAGGCCCTGGGCCAAGCCTGAAGATCCGTCTCTCCTGGAGGAT CCCAGGATCAAAGCAATTGCAGCCAAGTACAATAAAACTACAGCCCAGGTGCTGATCCGGTTCCCCATTC AGAGGAACTTGGTAGTGATCCCCAAGTCGGTGACACCAGTGCGCATTGCTGAGAACTTGAAGGTCTTTGA CTTTGAGCTGAGCAGCGAGGACATGGCCACTCTACTCAGCTACAACAGGAACTGGAGGGTGTGCGCCTTG ATGAGCTGTGCCAAACACAAGGATTACCCCTTCCACGCCGAAGTCTGAAGCTGTGGCGAGCGGATCCCTG CTCCTCCTCAAGTGGCTTGCACCTGTTCTTTCTGCCTCATCTGCCCTTGCAAGTGTCCTGTGTCTGCACT AGGTGGCACCTTGCAAACCAGATGGTGAGAGTTAGTTAGTTTGACATAGAGTCTGGAGGGCAGTACCAGT AGCCAAGGTGTTTCTTCCAGCCTTCCTTGGTCTTTCTTCTTACCCCCCTGGAGGACTTTAACACAAGTAC CCTTTCCAACCAAAGAGAAGCAAGCTTTAGAGTCCAAGCCGTGCCACTAACACTTAAATGTGAGTGCTTA GAACTGTAATCCTTTGGGTCAGACTTCTCCTTGCCTCAAATAAAAACTGCTTTTGTGAAAAAAAAAAAAA AA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009658 |
| Insert Size | 951 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC021655, AAH21655 |
| RefSeq Size | 1402 bp |
| RefSeq ORF | 951 bp |
| Locus ID | 11677 |
| UniProt ID | P45376 |
| Gene Summary | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols (PubMed:17381426, PubMed:19010934, PubMed:7851421). Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins (PubMed:19010934). Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides (PubMed:17381426). Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls) (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204522 | Akr1b3 (tGFP-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 2,850.00 |
|
| MR204522 | Akr1b3 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 2,400.00 |
|
| MR204522L3 | Lenti ORF clone of Akr1b3 (Myc-DDK-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 4,750.00 |
|
| MR204522L4 | Lenti ORF clone of Akr1b3 (mGFP-tagged) - Mouse aldo-keto reductase family 1, member B3 (aldose reductase) (Akr1b3) |
CNY 4,750.00 |
