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Pkm (NM_011099) Mouse Untagged Clone
CAT＃: MC203724
Pkm (untagged) - Mouse pyruvate kinase, muscle (Pkm2), (10ug)
CNY 5,944.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC016619
GTCCGCTCTAGGTATCGCAGCAGGAACCGAAGTACGCCCGAGGACTTCAGGAACCATGCCGAAGCCACAC AGTGAAGCAGGGACTGCCTTCATTCAGACCCAGCAGCTCCATGCAGCCATGGCTGACACCTTCCTGGAAC ACATGTGCCGCCTGGACATTGACTCTGCCCCCATCACGGCCCGCAACACTGGCATCATTTGTACCATTGG GCCTGCTTCCCGATCTGTGGAGATGCTGAAGGAGATGATTAAGTCTGGAATGAATGTGGCTCGGCTGAAT TTCTCTCATGGAACCCATGAGTACCATGCAGAGACCATCAAGAATGTCCGTGAAGCCACAGAAAGCTTTG CATCTGATCCCATTCTCTACCGTCCTGTTGCGGTGGCTCTGGATACAAAGGGACCTGAGATCCGGACTGG ACTCATCAAGGGCAGCGGCACCGCTGAGGTGGAGCTGAAGAAGGGAGCCACTCTGAAGATCACCCTGGAC AACGCTTACATGGAGAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACATCTGCAAGGTGGTGG AGGTGGGCAGCAAGATCTACGTGGACGATGGGCTCATCTCACTGCAGGTGAAGGAGAAAGGCGCTGACTT CCTGGTGACGGAGGTGGAGAATGGTGGCTCCTTGGGCAGCAAGAAGGGCGTGAACCTGCCGGGCGCTGCT GTGGATCTCCCCGCTGTGTCGGAAAAGGACATCCAGGACCTGAAGTTTGGGGTGGAGCAGGATGTGGACA TGGTGTTTGCATCTTTCATCCGCAAGGCAGCCGACGTGCATGAAGTCAGGAAGGTGCTGGGAGAGAAGGG CAAGAACATCAAGATCATCAGCAAAATCGAGAACCATGAAGGCGTCCGCAGGTTTGATGAGATCTTGGAG GCCAGTGATGGGATCATGGTGGCTCGTGGTGACCTGGGCATTGAGATTCCTGCAGAGAAGGTCTTCCTGG CTCAGAAGATGATGATCGGGCGATGCAACCGAGCTGGGAAGCCTGTCATCTGTGCCACACAGATGCTGGA GAGCATGATCAAGAAGCCACGCCCCACCCGTGCTGAAGGCAGTGATGTGGCCAATGCAGTCCTGGATGGA GCAGACTGCATCATGCTGTCTGGAGAAACAGCCAAGGGGGACTACCCTCTGGAGGCTGTTCGCATGCAGC ACCTGATTGCCCGAGAGGCAGAGGCTGCCATCTACCACTTGCAGCTATTCGAGGAACTCCGCCGCCTGGC GCCCATTACCAGCGACCCCACAGAAGCTGCCGCCGTGGGTGCCGTGGAGGCCTCCTTCAAGTGCTGCAGT GGGGCCATTATCGTGCTCACCAAGTCTGGCAGGAGTGCTCACCAAGTGGCCAGGTACCGCCCTCGGGCTC CTATCATTGCCGTGACTCGAAATCCCCAGACTGCTCGCCAGGCCCATCTGTACCGTGGCATCTTCCCTGT GCTGTGTAAGGATGCCGTGCTGAATGCCTGGGCTGAGGATGTCGACCTTCGTGTAAACTTGGCCATGGAT GTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCGGGTGGCGCCCTGGCT CTGGATTCACCAACACCATGCGTGTAGTGCCTGTACCTTGATGGCCCTCTGGAGCCCCTCTTCTAGCCCC TGTCCCTTCCCCTCCCCTATCCTTTCCATTAGGCCAGCAACGCTTGTAGTGCTCACTCTGGGCCATAGTG TGGCGCTGGTGGGCTGGGACACCAGGGAAAATTAATGCCTCTAAAACATGCAATAGAGACCAGCTATTAT TCAGGGCCCTACCTGAGCCAGGGGTGGAGGAGGAATGCAGGACTGGAAACCCTGACTTTATCACAGAAGG GCGGCAGCATCTCTGGGCTTTGCTTCTGTAGAAAGTTGTCAGAATTCCCAGCCCTAGCCTGGAGTCAGGA GACAGCAAAAGAGTAGGGGCTGAGGGTGTGGGGCCCAGGGTCCCAGTGTAGATGACGACTTCTGGCCCTG GCCCTGACCTGCTTTCCCAACAGCTTTGGCCTCCCCACTTCTTGTGCACTCCACTTCTGTCACTGCAGAC ACTCCACTCTCCACCTTGTATTCTGCAGAGTCTCCAGGCCTGTTGCTATAGTGCCCACCTGAATGTCAAT AAACAGCAGCTGAAGCACCTGTAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_011099 |
| Insert Size | 1596 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC016619, AAH16619 |
| RefSeq Size | 2212 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 18746 |
| UniProt ID | P52480 |
| Gene Summary | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (By similarity). Stimulates POU5F1-mediated transcriptional activation (By similarity). Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (PubMed:29996098).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an in-frame exon and contains an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (M2) is identical in length to isoform M1, but has a different sequence. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208518 | Pkm (tGFP-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 7,544.00 |
|
| MR208518 | Pkm (Myc-DDK-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 5,944.00 |
|
| MR208518L3 | Lenti ORF clone of Pkm (Myc-DDK-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 8,344.00 |
|
| MR208518L4 | Lenti ORF clone of Pkm (mGFP-tagged) - Mouse pyruvate kinase, muscle (Pkm2) |
CNY 8,344.00 |
