United States
Germany
Japan
United Kingdom
China
Bcl6 (NM_009744) Mouse Untagged Clone
CAT＃: MC203091
Bcl6 (untagged) - Mouse B-cell leukemia/lymphoma 6 (Bcl6), (10ug)
CNY 7,776.00
货期*
现货
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Bcl5 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC052315
CGGTGATGCAAGAAGTTTCTAGGAAAGGCCGGACACCAGTTTTAAAGCAAAATTTTGGACTGTGAAGCAA GGCACTGGGCAAACACAACATGGCCTCCCCGGCTGACAGCTGTATCCAGTTTACCCGGCACGCTAGTGAT GTTCTTCTCAACCTTAATCGCCTCCGGAGTCGGGACATCTTGACGGACGTTGTCATCGTGGTGAGCCGTG AGCAGTTTAGAGCCCATAAGACAGTGCTCATGGCCTGCAGCGGCCTGTTCTACAGTATCTTCACTGACCA GTTGAAATGCAACCTTAGTGTAATCAATCTAGATCCTGAAATCAGCCCTGAGGGGTTTTGCATCCTCCTG GACTTCATGTACACATCTAGGCTCAACCTGAGGGAAGGCAATATCATGGCGGTGATGACCACAGCCATGT ACCTGCAGATGGAGCATGTTGTCGACACATGCAGGAAGTTCATCAAGGCCAGTGAAGCAGAAATGGCCCC TGCACTTAAACCTCCCCGTGAAGAGTTCCTGAACAGCCGGATGCTGATGCCCCATGACATCATGGCCTAC CGAGGTCGTGAGGTCGTGGAGAACAATATGCCACTGAGAAATACTCCCGGGTGTGAGAGCAGAGCTTTTG CTCCTCCTCTGTACAGTGGCCTGTCAACACCACCAGCCTCTTATCCCATGTACAGCCATCTCCCGCTCAG CACCTTCCTCTTCTCTGATGAGGAGCTCCGAGATGCCCCCCGAATGCCTGTGGCCAACCCTTTTCCCAAG GAGCGTGCCCTCCCCTGCGACAGTGCCAGGCAAGTCCCTAATGAGTATAGCAGGCCAGCCATGGAGGTGT CCCCCAGTTTGTGTCACAGCAACATCTACTCGCCCAAGGAGGCAGTCCCAGAGGAGGCTCGGAGTGACAT ACACTACAGTGTGCCTGAGGGCCCCAAGCCTGCTGTCCCTTCTGCTCGGAATGCTCCATACTTCCCCTGT GACAAAGCCAGCAAAGAAGAAGAGAGACCTTCTTCGGAGGATGAGATTGCCCTGCATTTCGAGCCCCCCA ATGCACCCTTGAACCGGAAGGGTCTGGTTAGTCCCCAGAGTCCCCAGAAATCCGACTGCCAGCCCAACTC ACCCACAGAGTCCTGCAGCAGCAAGAACGCCTGCATCCTTCAGGCCTCTGGCTCTCCGCCAGCCAAGAGC CCCACTGACCCGAAAGCCTGCAACTGGAAGAAGTATAAGTTCATCGTTCTCAACAGCCTCAATCAGAATG CCAAACCCGAGGGCTCTGAGCAGGCAGAGCTGGGTCGCCTCTCCCCTCGAGCCTACCCTGCACCGCCCGC TTGCCAGCCGCCTATGGAGCCCGCGAACCTTGATCTCCAGTCCCCGACCAAGCTCAGTGCCAGTGGGGAG GACTCTACCATCCCCCAAGCCAGCCGGCTCAATAATCTCGTGAACAGGTCCCTGGCAGGCTCCCCCCGAA GCAGCAGTGAGAGTCACTCACCACTCTACATGCACCCCCCAAAGTGCACATCCTGCGGCTCTCAGTCCCC ACAGCATACAGAGATGTGCCTCCATACTGCTGGGCCCACGTTCCCGGAGGAGATGGGGGAAACCCAGTCA GAGTATTCGGATTCTAGCTGTGAGAATGGGACCTTCTTCTGCAACGAATGTGACTGCCGTTTCTCTGAGG AGGCCTCGCTCAAGAGGCACACGCTGCAGACGCACAGTGACAAACCATACAAATGTGATCGCTGCCAGGC CTCCTTCCGCTACAAGGGCAACCTCGCCAGCCACAAGACTGTCCACACGGGTGAGAAACCCTATCGCTGT AACATTTGTGGAGCGCAGTTCAATCGGCCAGCCAACCTGAAGACCCACACTCGAATTCACTCTGGAGAAA AGCCCTACAAATGTGAAACCTGTGGGGCCAGGTTTGTTCAGGTGGCCCACCTCCGTGCCCACGTGCTCAT CCACACTGGAGAGAAGCCGTACCCCTGTGAAATCTGTGGCACTCGCTTCCGGCACCTTCAGACTCTGAAG AGCCATCTGCGCATCCACACAGGAGAGAAACCTTACCATTGTGAGAAGTGTAACCTGCACTTTCGTCACA AAAGCCAACTGCGACTTCATTTGCGCCAGAAGCACGGCGCCATCACCAACACCAAGGTGCAATACCGCGT GTCGGCCGCTGACCTGCCTCCGGAGCTCCCCAAAGCCTGCTGAATGAAGCATGGAGTGTTCCTCGCCCTT TCCTCTCCAGCCCCTTCTCAGAATCTACCCAAAGGATGCTGTAACACTTTATACAAAGGTCATCCCATGA TGTAGTGCCTCTCTCATCCACTAGTGCAAATCATAGTTGGGGTGGGGGTGGGGGTGGGGTTTGCGGGACC GGGAGCCAAGGCAGCTCCCCTTCCCACACTGCCATAAAACATTAAGAAAATACTATTGCTTCTTCTCCTA TGTGTAAGGCAAACCCTGTCAGCAAAAAGCAAATTCATTTTCTATATCAAAGTAGGGGAGAATGCAGAAG TTCTGACTTGACAGGTTGCAACCGAGGAATGTAAATGATGTGTGGGAACAGAGGTCTCTTTTGTATGTAA ATGTGTATTGTTTTAAAAGACAAGACTTCAGTATGCTGTCAAAGAGAAGGCTTTAATTTTTTTAACCAAA GGTGAAGGAATATATGGCAGAGTTGTAAATATATAAATATATATATATATAATATAAATATATAAACCTA AAAAGATATATTGAAAATATAAAACTGTGTTAAAGGCTCGATTTTGTATCTGCAGGCAGACACGGATCTG AGAATCTTTATTGAGAAAGAGCACTTAAGAGACTATTTTAAGTATTGCGTCTGTATAAGTAAGAAAATAT TTTGTCTAAAATGCCTCCGTGTATTTGTATTTTTTTGCAAGTGAAGGTTTACAAATTTACAAAGTGTGTA TTAAAAACAAAAAGAACAAAAAAAAAAGCTGCAGAAGGAGAAATGTATACTTTTGTTCCAGTTTTCAGTT TGTACATACCTGTAATGTGTCCTCACGGTGCCTTTTTACACGGAAGTTTTCAATGATGGACGGGTGTGCG CCATCCCTTTTTGAAGTGTAGGCAGACACAGGGACTTGAAGTTGTCACTAACTAAACTCTCTTTGGGAAT GTTTGACTCCTCCCACATTCTGCGTCATGCTTGTCGTTATAATTACTCCGGAGACAGGGTTTGGCTGTGT CTAAACTGCATTAGTGCGTTGTAAAATAGAGCTGTACAAAACATAAGAATAAAACATTGAAAAGTCTTGA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009744 |
| Insert Size | 2124 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC052315, AAH52315 |
| RefSeq Size | 3330 bp |
| RefSeq ORF | 2124 bp |
| Locus ID | 12053 |
| UniProt ID | P41183 |
| Gene Summary | Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210168 | Bcl6 (tGFP-tagged) - Mouse B-cell leukemia/lymphoma 6 (Bcl6) |
CNY 7,700.00 |
|
| MR210168 | Bcl6 (Myc-DDK-tagged) - Mouse B-cell leukemia/lymphoma 6 (Bcl6) |
CNY 7,776.00 |
|
| MR210168L3 | Lenti ORF clone of Bcl6 (Myc-DDK-tagged) - Mouse B-cell leukemia/lymphoma 6 (Bcl6) |
CNY 8,930.00 |
|
| MR210168L4 | Lenti ORF clone of Bcl6 (mGFP-tagged) - Mouse B-cell leukemia/lymphoma 6 (Bcl6) |
CNY 8,930.00 |
Customer
Reviews
Loading...
