Glb1 (NM_009752) Mouse Untagged Clone

CAT#: MC202893

Glb1 (untagged) - Mouse galactosidase, beta 1 (Glb1), (10ug)



  "NM_009752" in other vectors (4)

CNY 4,824.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW125515; Bg; Bge; Bgl; Bgl-; Bgl-e; Bgl-s; Bgl-t; Bgs; Bgt; C130097A14Rik
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC028875 sequence for NM_009752
CTGCCTAACGGAGAGACCCCATCGTGGCGCGATCATGCTCCGGGTCCCCCTGTGTACGCCGCTCCCGCTC CTGGCACTGCTGCAACTGCTGGGCGCTGCGCACGGCATCTATAATGTCACCCAGAGGACATTTAAGCTCG ACTACAGCCGGGACCGCTTCCTCAAGGATGGACAGCCATTCCGATACATCTCGGGAAGCATTCATTACTT CCGGATACCCCGCTTCTACTGGGAGGACCGGCTGCTGAAGATGAAGATGGCTGGGCTGAATGCTATCCAG ATGTACGTGCCCTGGAACTTCCATGAACCCCAACCAGGACAATATGAGTTTTCTGGGGACCGTGATGTGG AGCATTTCATCCAGCTGGCTCATGAGCTGGGACTCCTGGTGATCCTGAGGCCTGGGCCCTACATCTGTGC AGAGTGGGACATGGGGGGCTTACCTGCTTGGCTACTAGAGAAACAATCTATCGTTCTCCGGTCTTCTGAC CCAGACTACCTTGTAGCTGTGGATAAATGGCTGGCAGTCCTTCTGCCCAAGATGAAGCCCCTGCTCTACC AGAACGGAGGACCGATCATAACCGTGCAGGTTGAGAATGAGTACGGGTCCTACTTTGCCTGCGATTACGA CTACCTACGCTTCCTGGTGCACCGCTTCCGCTACCATCTGGGTAATGACGTCATTCTCTTCACCACCGAC GGAGCAAGTGAAAAAATGCTGAAGTGTGGGACCCTGCAGGACCTGTACGCCACAGTGGATTTTGGAACAG GCAACAATATCACACAAGCTTTCCTGGTCCAGAGGAAGTTTGAACCTAAAGGACCTTTGATCAATTCTGA GTTCTATACTGGCTGGCTAGACCACTGGGGTAAACCCCATTCCACGGTGAAAACTAAAACACTGGCTACC TCCCTCTATAACCTGCTTGCCCGTGGGGCCAACGTGAACTTGTACATGTTTATAGGTGGGACCAATTTTG CCTATTGGAATGGTGCCAACACGCCCTATGAGCCACAGCCCACCAGCTATGACTACGACGCCCCACTGAG CGAGGCTGGGGACCTCACTAAGAAGTATTTTGCTCTTCGAGAAGTCATTCAGATGTTTAAAGAAGTCCCA GAAGGCCCTATCCCTCCGTCTACACCCAAATTTGCATATGGAAAAGTTGCTCTGAGAAAGTTCAAGACAG TGGCTGAAGCTCTGGGTATCCTGTGTCCCAATGGGCCAGTGAAAAGCCTCTATCCCCTGACATTCACTCA GGTAAAACAGTATTTTGGGTATGTGCTGTACCGAACAACGCTTCCTCAAGATTGCAGTAACCCGAAACCC ATTTTCTCTTCACCCTTCAATGGCGTCCGTGATCGGGCTTACGTCTCTGTGGACGGGGTCCCCCAAGGAA TCCTTGATCGAAACCTCATGACAGCTCTGAACATACAGGGGAAGGCTGGAGCCACGCTGGACATCCTGGT GGAGAACATGGGGCGTGTGAACTATGGCAGATTCATCAATGACTTCAAGGGTTTGATTTCCAACATGACT ATCAACTCCACTGTCCTCACCAACTGGACGGTCTTCCCACTGGACACTGAGGCCATGGTACGCAACCATC TCTGGGGCCGGGAGGCCAGTGATGGGGGTCACCTTGACGGACGGTCGACCTCCAATTCTTCGGACCTCAT ACTCCCCACCTTTTACGTGGGCAACTTCTCCATCCCCTCGGGCATCCCAGACCTGCCACAGGACACCTTC ATCCAGTTTCCTGGGTGGTCCAAGGGTCAAGTATGGATCAATGGCTTTAACCTCGGCCGATACTGGCCCA CAATGGGCCCACAAAAGACCTTGTTCGTGCCAAGGAACATCCTGACCACTTCAGCCCCAAACAACATCAC AGTGTTGGAGCTAGAGTTTGCACCCTGCAGCGAGGGGACCCCAGAGCTGTGTACAGTAGAGTTTGTTGAC ACTCCGGTCATTTCCTGACCTGACTTGACCATCGGTGGCCATTTTCCAAGCCAGTCTGGTCAAGACTCAT GGCTGAACCTCTGAGACTGAGCCTTGGGGAGCACAGCTCTACTCTGGTTACACGGATCACCTTTGTTGTG CTAGAATGGAAGCTACATTTCTGGAATGTGTGTACCCTGCTGGTGACTTTGCAGCCCCGCCTTGCCTGAC CTCCCACCCTAGTGGCCACCGGGAAGGCTGAAGGGAGTGGACACCACAGGAAGACACAGGTTGGAGCAAA ACTTGAATAATGTCCTTTATCCTGATTTGAAATAATCACGTCATCTTTCTGCTGAATAAAATTGGTTTGC AAGTTGAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_009752
Insert Size 1944 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC028875, AAH28875
RefSeq Size 2338 bp
RefSeq ORF 1944 bp
Locus ID 12091
UniProt ID P23780
Gene Summary This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a lysosomal enzyme that catalyzes the hydrolysis of terminal beta-D-galactose residues in various substrates like lactose, ganglioside GM1 and other glycoproteins. Mutations in the human gene are associated with GM1-gangliosidosis and Morquio B syndrome. Disruption of the mouse gene mirrors the symptoms of human gangliosidosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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