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Tpx2 (NM_028109) Mouse Untagged Clone
CAT＃: MC202638
Tpx2 (untagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2), transcript variant 2, (10ug)
CNY 2,000.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2610005B21Rik; DIL2; p100; REPP86 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC060619 sequence for NM_028109
GGACTCGTAGGGTTGGTGCAAGCTGAAACCGGGAAAGTAGCGTGAAGGTGAGGGGAGGCAGCGGTGCGCC GGAGGCCGTTTTTCCAGGGTTCTGAGAGGGCAGGGGAGTCCCCCGGGCGGGTCCGGACAGCCTAGACAGC GCGCGGGCTTTGGACGCCGGTGACAGGACTTCGGGGCGTTGGTATTTCTGGGAACCCCGGAGCAGAGGCT GCTCCAGGCTGTCAGCCACTAGACGGTTCTTCATGAGAAGTGTCTGCCAGACTGCAAGAATTCAAGAGAC TTGAGAACTTGATTGTGGCTTTGAGCGAGGTTGTCAGAAGACAGTCTATTGAGGAGAGTATTAGAATCCT GGAAAAAGCTTGTTTTTCTGCACTAAGTTGGGGCAATGTCACAAGTCCCTACTACTTACTCTTTCGATGC CCCCACCGACTTTATCAATTTTTCATCTTTGGATGCTGAAGAGGATACTGAAAATATAGACTCATGGTTT GATGAGAAGGCCAACTTGGAGAACAAGTTTCTTCGACAGAGGGGAATAGGCGAGCCTTTTCAGGGGAAGA ATTCCTTGAGAAAAGCCAAACTTCAACAGGGCTTCGTCACACCGTTGAAGGCAGTTGACAACACTTACCA CAAAGAGACAGAAAAGGAAAATCTTCAGAAACAGTCTATCCCATCAAATGATTGTTCTTCCCTGGATGCT AAGAGAGCTGTATCAGGAAATACTCCTGTCCAGCCTCAGAGAAGATCTATTAGACTCTCTGCTCAGAAGG ATTTGGAGCAGAAAGAGAAAAACCATGTTGCCTCTGTTGAAATGAAAGCCAAGAGATGTGTTGCTCCGGC CACTGATTGTCCCCCCCAGAAAAGAATGAAAGTTTCTGATAAAAAGAAACTGGAGGAAGAGGAGGAAGGC AGTGCTCCAGCTACTTCAAGAAAGAATGAAAGAGAAACCCTGGAGAAAGCCAAGGGCAAGCACACTGTGC CAGGTGTGCCACCTGCAAGGGAGAAGGTTCTAAAGAGTACTGAGGAGCAGGAGATAGAGAAAAGGCTGCG GATGCAGCAGGAGGTGGTGGAGCTGCGCAGGAAAAACGAAGAGTTCAAGAAGCTCGCGCTCGCAGGGCCA GGGCAACCTGTGAAGAAGTCCACGAGCCAGGTTACCAAGACAGTTGACTTTCACTTCCTCACAGATGAGC GAATCAAGCAACATCCCAAGAACCAGGAAGAGTATAAGGAAGTGAACTTCATGTCTGAACTTCGGAAGCA TTCTTCCACGCCTGCCCGAGGAACCAGAGGATGCACTATCATTAAGCCTTTCAACCTGTCCAAAGGGAAG AAAAGAACATTTGATGAAGCAGCTTCTACGTATGTGCCCATTGCACAGCAGGTTGAAGCCTTCCACAAAC GAACCCCCAATAGATACCATCTGAGGAACAAGAAGGACGAGAGCTTGTTACCCTCCAAATCTGTGAACAA GATTGCACGAGACCCCCAGACCCCCATACTGCAGACCAAATATCGTACAAGGGCTGTGACTTGCAAAAGT ACTGCAGAGCAGGAGGCCGAGGAGCTTGAGAAACTGCAACAATACAAATTCAAAGCACGGGAACTTGATC CTAGAATTTTTGAAAGTGGCCCCATCTTGCCCAAGAGAGCACCTGTTAAACCTCCTACTCAGCCTGTTGG TTTTGATTTGGAAATTGAGAAACGAATTCATGAGCGAGAGTCAAAGAAAAAAACAGAAGATGAACAATTT GAATTTCATTCTAGACCTTGTCCTACTAAGATCTTGGAAGATGTCGTGGGTGTTCCTGAAAAGAAGGTAA TTCCAGCTACTGTCCCGAAGTCGCCAGTTTTTGCATTGAAGAACAGGATCCGAGTGCCCATCAAAGATGA GGAAGAGGAGAAACCAGTAGTGATAAAAGCTCAACCTGTGCCACATTATGGGGTGCCTTATAAGCCCCAC ATCGCAGAGGCAAGAAATGTGGAGGTGTGCCCGTTCTCCTTTGATACCCGGGACAAAGAACGCCAGTTGC AGAAGGAGAAAAAAATAAAAGAGATGCAGAAAGGGGAGGTGCCCAAGTTCAAGGCACTTCCTGTGCCCCA TTTTGACACCATTAACCTGCCAGAGAAAAAGGTAAAGAACGTGACTCAAGCTGAGCCTTTCTCCCTGGAG ACAGACAAGAGAGGCGCCTACAAGGCTGAGATGTGGAAGCACCAGCTGGAGGAAGAGCAGAAGCAGCAGA AGGACGCCGCTTGCTTCAAGGCTCGTCCAAACACCGTCATCTTCCAAGAGCCCTTCGTTCCCAAGAAGGA GAAAAAATCACTGGCTGAGAACCCTTCTGGTTCTCTAGTTCAGGAACCTTTTCAGCTGGCCACCGAGAGG AGAGCCAAAGAGCGGCAGGAGCTGGAGAAGAAAATGGCTGAAGTAGAGGCCTGGAAACTGCAGCAGTTGG AGGAAGTCAGGCAGCAGGAAGAGGAGCAGCAGAAGGAGGAGTTAGCCAGGCTCCGGAAAGAACTGGTGCA CAAGGCAAATCCAATACGGAAGTACGCAGCAGTGGAGGTGAAATCTAGCGAGCTGCCTCTGACTGTGCCG GTGTCTCCTAAGTTCTCCACTCGGTTCCAGTAGAAGCTGCATCTATATCACAAGCATGGAGCACGCGTCT TCAGACTCACCCGCCATGTGTGGAAACACACCTGATAACTAATGGATGCCGGGTGTTTTGAAGATGACTC TACACAGCAACTTTGAAGCTAATGAGCCTCCACTCATTTAGACTTTAACCAGACTTCTTGTAGTTATTTC CTTCAGACATTAGCCGGCCCTTTCCTGTTGGTCTTAACGCTGGCTAGGATTTCAAGAATTCGTTTTGGCA TAGTCTACTTTTCACCTAATCCCCCCTTATTACCCCTTATATATGTATTTTAAGCATAAAATAAAGGCAG TCAATTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_028109 |
| Insert Size | 2238 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC060619, AAH60619 |
| RefSeq Size | 2994 bp |
| RefSeq ORF | 2238 bp |
| Locus ID | 72119 |
| UniProt ID | A2APB8 |
| Gene Summary | Spindle assembly factor required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation. TPX2 is inactivated upon binding to importin-alpha. At the onset of mitosis, GOLGA2 interacts with importin-alpha, liberating TPX2 from importin-alpha, allowing TPX2 to activates AURKA kinase and stimulates local microtubule nucleation.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210424 | Tpx2 (tGFP-tagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2) |
CNY 6,370.00 |
|
| MG221964 | Tpx2 (tGFP-tagged) - Mouse TPX2 microtubule-associated protein homolog (Xenopus laevis) (Tpx2) transcript variant 2, (10ug) |
CNY 6,370.00 |
|
| MR221964 | Tpx2 (Myc-DDK-tagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2), transcript variant 2 |
CNY 5,464.00 |
|
| MR221964L3 | Lenti ORF clone of Tpx2 (Myc-DDK-tagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2), transcript variant 2 |
CNY 7,700.00 |
|
| MR221964L4 | Lenti ORF clone of Tpx2 (mGFP-tagged) - Mouse TPX2, microtubule-associated protein homolog (Xenopus laevis) (Tpx2), transcript variant 2 |
CNY 7,700.00 |
