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Rrp8 (NM_025897) Mouse Untagged Clone
CAT＃: MC202542
Rrp8 (untagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2, (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1500003O22Rik; 2900001K19Rik; AW538116 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC046799 sequence for NM_025897
CCCGGTTTGGTCCGACTCCAGCCCTAGGATTCCCTGACGCGCGCTGCACGTGGGCCAGAGCGTAGGCTCC TCCGGCCCTGCGGGCTACGGGTGGTATGTTCGAAGAGCCCGAATGGGTGGAGGCGGCCCCAGCCATCGTG GGCCTAGGGGCCGCGACAGCACAGGTTAGGCCGGCGACTGCCCCGCCAGTCAAGGGACGCAAGCGCCGCC ATCTCTTGGCCACATTACGGGCTCTGGAAGCAGCATCTCTCTCCCAACAAACCCCCAGCCTACCTGGCAG TGACTCTGAGGAGGAGGAGGAGGTAGGAAGGAAGAAGAGACACCTCCAAAGGCCCTCACTTGCCAGCGTC TCAAAGGAAGTAGGGAAGAAAAGAAAAGGGAAATGTCAGAAACAGGCGCCATCCATCAGTGACTCTGAGG GGAAAGAAATAAGAAGAAAATGCCACAGACAAGCTCCTCCTCTTGGTGGGGTCTCTGCTGGAGAAGAAAA AGGAAAGAGAAAATGCCAGGAATATTCCTCTTTACACCTAACCCAGCCCCTGGACAGTGTTGACCAAACA GTTCACAATTCCAGGACGAGTACTGCTACAATTGACCCATCCAAGCCAAGCCCTGAGTCTATGTCACCTA ACTCCTCACACACCCTGAGCCGCAAGCAGTGGCGGAACCGGCAGAAAAATAAGCGGAGACACAAGAACAA ATTTCGGCCACTCCAGACACCAGAGCAGGCTCCTCCCAAGGCTTCCATAGAGGAGACTGAGGTGCCTCCT GTCCCAAAGTCAGATAGTCAAGAGTCTAGAGCTGGAGCCCTGCGAGCACGCATGACACAACGCCTGGATG GGGCCCGATTTCGCTACCTTAATGAACAGTTGTACTCAGGGCCCAGCAGTGCTGCCCGACGCCTATTCCA GGAAGACCCTGAGGCTTTTCTCCTTTATCACCGTGGCTTCCAGAGACAAGTAAAGAAGTGGCCACTGCAC CCAGTGGACCGTATTGCCAAAGATCTCCGCCAGAAGCCTGCATCCTTAGTGGTAGCTGACTTTGGCTGTG GAGATTGCCGCCTAGCTTCAAGTGTCCGGAACCCTGTGCACTGTTTTGATTTGGCTTCTCTGGACCCCAG GGTCACAGTATGTGACATGGCCCAGGTGCCTCTGGAGGATGAATCTGTAGATGTGGCTGTGTTTTGCCTT TCACTGATGGGAACTAACATCAGGGACTTCCTTGAGGAGGCAAATCGAGTGCTAAAAACAGGGGGTCTTC TCAAAGTAGCTGAAGTCAGCAGCCGCTTTGAGGATATTCGGACCTTTTTGGGGGCTGTGACCAAACTCGG CTTTAAGATTATCTACAAGGACCTGACCAACAGCCACTTCTTCTTGTTTGACTTTGAAAAGACCGGACCT CCTCGAGTAGGACCCAAAGCCCAACTCTCAGGCCTTAAACTTCAGCCCTGTCTCTACAAGCGCAGGTGAC CGATGGACACCCATTGGAAGGGGAGAGCTCAGACTCCAGTCTCAGAACTCTGAAGACTGTATCTACCCAG GCTATGAGCCAGGATCTGGTACCACCCTCCTTCTGTACCAAGAACACTGTGGTAAAGCCTCCAGCTCAGC TCTACTCTAATGGAGCCTTCTTGGATACAGAAAGCATAAAATTACTTGGGCAGGCTCAAGAATGAGGTGT TTATTGTGAGGGCATGGATGTGTCTAATAATTGTGGGTCACTTCAGTATTGTGGAAACTAGTCGGCTGAG AACTGATGGATCTCTGGGTTTGCTTTCTCTTCATCTCTCAGAAGGCACGTGGGCTCTCCGCTGTGTTGTC TTCTGACCAATATTGTCCACCTCTGCTCATCCATAGTTTCTGCTCCTTCAGCTTCAAGACTACAGACAGG CAGTTTTTGCCTTATGGCAGGTCCCAATGCTAGTTTGCCACTTGGGGCTGGGAGTTATTTAATTTCTTTG TTTCTTGGAGCTGGGAATCATCTAATTTTTTTCTGTGTTGCTATTGTTGTTGTTGTTGTTGTTGTTTTGA GACAGTATCTCACACTAGTCCAAGCTGACCTCAAACAAAACAAATGGCAATCCTCCTGCCTCAGCCTCCT GATTATTGGGAACACAGGCATGGGCCACCATATTTAGCTGAAGAAATCATTTACTTTCTGAATATTCTAT TTCTCATTTGAAAAATGTAACCTTCTTGTCTTACAGTATGTTAAATGGGTTCACTGAGGAAAGCTACAGC TAGAGCTGGGACTAGTAAGAGGTGCTGAATAAACCAGCTATGGCTAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_025897 |
| Insert Size | 1374 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC046799, AAH46799 |
| RefSeq Size | 2324 bp |
| RefSeq ORF | 1374 bp |
| Locus ID | 101867 |
| UniProt ID | Q9DB85 |
| Gene Summary | Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and 5' coding region, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207304 | Rrp8 (tGFP-tagged) - Mouse RIKEN cDNA 1500003O22 gene (1500003O22Rik) |
CNY 5,040.00 |
|
| MR207304 | Rrp8 (Myc-DDK-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2 |
CNY 4,560.00 |
|
| MR207304L3 | Lenti ORF clone of Rrp8 (Myc-DDK-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2 |
CNY 6,460.00 |
|
| MR207304L4 | Lenti ORF clone of Rrp8 (mGFP-tagged) - Mouse ribosomal RNA processing 8, methyltransferase, homolog (yeast) (Rrp8), transcript variant 2 |
CNY 6,460.00 |
