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Col3a1 (NM_009930) Mouse Untagged Clone
CAT＃: MC202519
Col3a1 (untagged) - Mouse collagen, type III, alpha 1 (Col3a1), (10ug)
CNY 10,024.00
货期*
现货
规格
| Cited in 1 publication. |
Product images
经常一起买 (3)
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW550625; Col3; Col3a-1; Tsk; Tsk-; Tsk-2; Tsk2 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC058724 sequence for NM_009930
ACCATTCTGATGTTGGAGCGGCCGCGTGCTGAAGGGCAGGGACAACTGATGGTGCTACTCTGAGCTGCTT CTTCCTCTCTCCTCTTTTGCACAAAGAGTCTCATGTCTGATATTTAGACATGATGAGCTTTGTGCAAAGT GGAACCTGGTTTCTTCTCACCCTTCTTCATCCCACTCTTATTTTGGCACAGCAGTCCAACGTAGATGAAT TGGGATGCAGCCACCTTGGTCAGTCCTATGAGTCTAGAGATGTCTGGAAGCCAGAACCATGTCAAATATG TGTCTGTGACTCAGGATCTGTCCTTTGCGATGACATAATCTGTGATGAGGAGCCACTAGACTGCCCCAAC CCAGAGATCCCATTTGGAGAATGTTGTGCAATATGCCCACAGCCTTCTACACCTGCTCCTGTGCTTCCTG ATGGCCATGGACCTCAAGGCCCCAAGGGAGATCCGGGTCCTCCTGGCATTCCTGGGAGAAATGGTGACCC TGGCCTTCCAGGACAACCAGGTCTCCCTGGGCCTCCTGGCTCCCCTGGAATCTGTGAATCATGTCCAACT GGTGGCCAAAATTATTCTCCCCAATTCGACTCATATGATGTCAAGTCTGGAGTGGGAGGAATGGGTGGCT ATCCGGGACCAGCTGGTCCACCAGGCCCTCCAGGACCCCCTGGTTCTTCTGGACATCCTGGATCTCCTGG TTCTCCTGGATACCAAGGTCCCCCTGGTGAACCTGGTCAAGCTGGTCCGGCAGGCCCTCCAGGACCTCCT GGTGCTCTTGGTCCAGCTGGTCCTGCTGGAAAGGATGGAGAGTCAGGAAGACCTGGACGACCTGGAGAGC GTGGACTGCCTGGACCTCCAGGTATCAAAGGCCCAGCTGGCATGCCTGGATTCCCTGGTATGAAAGGACA CAGAGGCTTTGATGGACGCAATGGAGAAAAGGGAGAAACAGGTGCTCCTGGACTGAAGGGTGAAAATGGT CTTCCAGGAGACAACGGAGCTCCTGGCCCCATGGGTCCTAGAGGGGCTCCTGGTGAGCGAGGACGACCAG GCCTTCCTGGAGCTGCAGGTGCTCGAGGCAATGATGGTGCTCGGGGCAGTGATGGGCAACCTGGTCCCCC TGGCCCTCCTGGAACTGCAGGATTCCCTGGATCCCCTGGTGCTAAGGGTGAAGTTGGACCTGCAGGGTCC CCTGGCTCAAATGGCTCACCAGGACAAAGAGGGGAGCCTGGACCGCAGGGACATGCTGGTGCTCAAGGGC CTCCTGGCCCTCCCGGGAATAACGGCAGTCCTGGTGGAAAGGGTGAAATGGGTCCAGCTGGCATTCCTGG AGCCCCTGGACTAATAGGAGCAAGGGGCCCCCCTGGACCAGCAGGAACTAATGGTATACCCGGAACACGA GGTCCTTCAGGTGAACCCGGCAAGAACGGTGCGAAGGGAGAGCCAGGAGCACGCGGTGAACGGGGCGAAG CTGGTTCCCCAGGAATTCCAGGACCTAAGGGCGAAGATGGCAAAGATGGATCACCTGGAGAACCTGGTGC AAATGGGCTTCCAGGAGCCGCAGGAGAAAGGGGTCCTAGTGGCTTCCGAGGACCTGCAGGGCCAAATGGC ATCCCAGGAGAAAAGGGTCCTCCCGGAGAGCGTGGTGGCCCAGGTCCTGCAGGACCCAGAGGAGTAGCTG GAGAACCTGGCCGAGATGGAACCCCTGGAGGTCCAGGGATACGGGGTATGCCCGGAAGTCCAGGTGGACC AGGCAATGATGGAAAACCAGGACCTCCTGGAAGTCAAGGAGAAAGTGGTCGCCCTGGTCCCCCTGGCCCA TCTGGTCCCAGAGGTCAGCCTGGTGTCATGGGTTTCCCTGGTCCTAAAGGAAATGATGGTGCCCCTGGCA AGAATGGAGAACGGGGTGGCCCTGGAGGACCTGGCCTTCCAGGTCCTGCTGGAAAGAATGGGGAGACTGG ACCTCAGGGTCCCCCAGGACCTACTGGCCCAGCTGGTGACAAGGGAGATTCTGGACCCCCTGGTCCACAA GGATTACAAGGCATACCTGGTACCGGTGGTCCTCCAGGAGAAAATGGGAAACCAGGTGAACCAGGTCCAA AGGGTGAAGTCGGTGCTCCTGGAGCTCCTGGAGGCAAGGGTGATTCTGGTGCACCTGGAGAACGTGGGCC TCCTGGAACAGCAGGAATCCCTGGTGCTAGAGGTGGAGCTGGACCCCCTGGCCCTGAGGGAGGAAAGGGC CCTGCTGGTCCCCCTGGCCCTCCTGGTGCTTCTGGTTCTCCTGGTCTGCAAGGGATGCCAGGGGAAAGAG GAGGTCCTGGAAGTCCTGGCCCAAAGGGTGAAAAGGGTGAACCAGGTGGTGCAGGTGCTGACGGAGTTCC AGGAAAGGATGGGCCAAGGGGTCCTGCTGGCCCTATTGGTCCCCCTGGCCCAGCTGGTCAGCCTGGAGAT AAGGGTGAAGGTGGTTCCCCTGGACTTCCAGGTATAGCTGGACCTCGAGGTGGCCCTGGTGAGAGAGGTG AACATGGCCCTCCAGGACCTGCTGGCTTCCCGGGTGCTCCTGGACAGAATGGTGAACCAGGTGCTAAAGG AGAAAGAGGTGCCCCTGGAGAGAAAGGTGAAGGAGGCCCTCCAGGACCTGCAGGACCCACTGGCAGTTCT GGACCTGCTGGTCCGCCTGGTCCTCAGGGTGTAAAGGGTGAACGTGGTAGTCCTGGTGGTCCTGGTACTG CTGGCTTTCCCGGTGGGCGTGGTCTTCCTGGTCCTCCTGGCAATAATGGTAATCCAGGGCCCCCAGGACC CAGTGGTGCTCCTGGCAAGGACGGCCCTCCAGGTCCTGCAGGCAACAGTGGTTCTCCTGGCAACCCTGGA ATAGCTGGACCAAAAGGTGATGCTGGACAGCCTGGAGAGAAGGGGCCACCTGGTGCTCAGGGTCCTCCGG GTTCCCCAGGCCCACTTGGAATTGCAGGGCTAACTGGAGCACGAGGTCTTGCTGGACCACCAGGCATGCC AGGTCCACGGGGTAGCCCTGGACCTCAGGGTATCAAGGGTGAAAGTGGAAAACCAGGAGCCAGTGGCCAT AATGGGGAACGTGGTCCTCCTGGACCCCAAGGTCTTCCTGGTCAGCCTGGTACAGCTGGTGAACCTGGAA GGGATGGAAACCCTGGATCAGATGGTCAGCCAGGTCGAGATGGATCTCCTGGTGGCAAGGGTGATCGTGG TGAAAATGGTTCTCCTGGTGCCCCAGGCGCTCCTGGTCATCCAGGCCCACCTGGCCCTGTTGGTCCATCT GGAAAGAGTGGTGACAGAGGAGAAACTGGCCCTGCTGGTCCTTCTGGTGCTCCAGGCCCTGCTGGAGCTC GAGGTGCTCCGGGTCCCCAAGGTCCACGAGGTGACAAAGGTGAAACTGGTGAACGTGGCTCTAATGGCAT CAAAGGACATCGAGGATTCCCTGGCAATCCAGGTCCTCCAGGTTCTCCTGGTGCTGCTGGTCACCAGGGT GCAATTGGTAGTCCAGGACCTGCAGGTCCCAGAGGACCAGTTGGACCACATGGACCTCCTGGAAAAGATG GAACAAGTGGGCATCCAGGTCCTATTGGACCACCAGGTCCTAGAGGAAACAGAGGTGAAAGAGGATCTGA GGGCTCGCCAGGCCACCCTGGACAGCCAGGACCCCCTGGACCCCCTGGTGCCCCTGGTCCCTGCTGTGGT GGTGGTGCTGCTGCCATTGCTGGAGTTGGAGGTGAAAAGTCTGGTGGCTTTTCACCCTATTATGGAGACG ATCCAATGGATTTCAAGATCAACACTGAAGAGATTATGTCTTCACTCAAGTCTGTTAATGGACAAATAGA GAGTCTTATAAGCCCTGATGGTTCTCGAAAAAACCCTGCTCGGAACTGCAGAGACCTAAAATTCTGCCAC CCCGAACTCAAGAGTGGAGAATACTGGGTTGATCCTAACCAAGGCTGCAAGATGGATGCTATAAAAGTAT TCTGTAACATGGAAACTGGGGAAACATGCATAAATGCCAGCCCCATGACTGTCCCACGTAAGCACTGGTG GACAGATTCTGGTGCAGAGAAGAAACATGTTTGGTTTGGAGAATCTATGAATGGTGGTTTTCAGTTCAGC TATGGCCCTCCTGATCTTCCTGAAGATGTCGTTGATGTGCAGCTGGCATTCCTCAGACTTCTTTCCAGCC GGGCCTCCCAGAACATTACATACCACTGCAAAAACAGTATTGCCTATATGGATCAGGCCAGTGGCAATGT AAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAATTCAAGGCTGAAGGAAACAGCAAATTCACT TACACAGTTCTAGAGGATGGCTGTACTAAACACACTGGGGAATGGAGCAAGACAGTCTTTGAATATCAAA CACGCAAGGCAATGAGACTACCCATCATAGATATCGCACCCTATGACATTGGGGGTCCTGATCAAGAATT TGGTGTGGACATTGGCCCTGTTTGCTTTTTATAAGCCAAACTCTCTGAAACCCCAGCAAAACAAAAACCA CATCCATGTGTTCATCTTGTTTTAATCTTATCAACCAGTGCAAGTGACCAACTAAATTCCAGTTATTTAT TTCCAAACTTTTGGAAAAAGCATAATTTGACAAAAAAAGAATACAATTTTTTGCTGTTTCAACCACCCAA TACAGGTCAAATGCTTTTGTTTTATTTTTTTACCAATTCCAACTTCAAAATGTCTCAATGGTGCTATAAT AAATAAACGTCAACACTTTTATGATAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | AscI-NotI |
| ACCN | NM_009930 |
| Insert Size | 4395 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC058724, AAH58724 |
| RefSeq Size | 4812 bp |
| RefSeq ORF | 4395 bp |
| Locus ID | 12825 |
| UniProt ID | P08121 |
| Gene Summary | This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The Tsk2/+ Mouse Fibrotic Phenotype Is Due to a Gain-of-Function Mutation in the PIIINP Segment of the Col3a1 Gene
,Long, KB;Li, Z;Burgwin, CM;Choe, SG;,
Journal of Investigative Dermatology
[COL3A1]
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