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Klhl25 (NM_182782) Mouse Untagged Clone
CAT＃: MC202166
Klhl25 (untagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3, (10ug)
CN¥ 4,392.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2810402K13Rik; BC027373; Enc-1 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC027373 sequence for NM_182782
CGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGCGGACGCGTGGGGGGATCGCGG CCGGGACTGGTGAGCCGGCCGGGGGCGGCGGCAGCGGCGGGGACTGCAGAGCCTGTGGGGCCCGCACGGT GAGGACCAGGTTGGTACACGATGTCAGTCAGTGTTCACGAGACCCGCAAGTCGAGGAGCAGCACGGGCTC CATGAACATCTCAGTCTTCCACAAGGCTTCACACCCTGACTGTGTGCTGGCCCACCTCAACACTCTGCGC AAGCACTGCATGTTCACGGATGTCACACTCTGGGCTGGTGACCGAGCCTTTCCTTGCCATCGTGCGGTGC TGGCTGCCTCCAGCCGATACTTTGAGGCCATGTTCAGCCACGGCCTGCGGGAAAGCCGGGATGATACGGT CAACTTCCAGGACAACCTGCACCCCGAGGTGTTGGAGCTGCTGTTGGACTTTGCCTACTCTTCTCGCATC GTCATCAATGAGGAGAACGCGGAGTCACTACTAGAGGCTGGTGACATGCTGCAGTTCCATGATGTCCGGG ATGCAGCTGCTGAGTTCCTGGAGAAGAATCTTTCCCCCTCCAACTGCCTTGGCATGATGGTGCTGTCTGA TGCCCACCAGTGCCGACGGCTCTACGAGTTCTCATGCCGAATGTCCCTGGTGCATTTTGAGACGGTGCGG CAGAGCGAGGACTTCAACAGTCTCTCTAGGGACACGCTGTTGGACCTCATCTCCAGAGACGAGCTGGAGA CTGAGGATGAGCGCGTGGTCTTTGAGGCCATCCTGCAGTGGGTGAAGCATGACTTAGAGCAGAGGAAGGC CCACCTGCCCCTGCTCCTGCGTAACGTGCGACTGGCCCTGCTGCCCTCAGACTGCCTGAAGAATGCCGTA TCTGGAGAGGCCCTCCTCATGGCAGACGAGTGCACCAAGCTCATCTTAGACGAGGCCTTCCGCTGCAAGA CCAAGATCTTGCTGAATGATGGTGTGGTCACCAGTCCCTTTGCCCGGCCTCGCAAGGCAGGTCACACACT GCTCATCTTGGGAGGCCAGACCTTCATGTGTGACAAGATCTACCAAGTGGACCACAAAGCCAAGGAGATT ATCCCCAAGGCAGATCTGCCAAGCCCCCGGAAGGAGTTCAGTGCTTCAGCGATTGGTTGCAAGGTCTATG TGACTGGAGGCAGGGGCTCTGAGAATGGAGTCTCTAAGGATGTCTGGGTGTATGACACTGTCCATGAGGA ATGGTCTAAGGCCGCCCCTATGCTGATTGCCCGCTTTGGCCATGGCTCAGCCGAGCTGGAGAACTGTCTC TATGTGGTGGGGGGGCATACATCCCTAGCAGGTATTTTCCCTGCCTCCCCTTCTGTCTCCCTGAAGCAAG TAGAGAAATATGACCCTGGGGATAATAAGTGGACTATGGTGGCCCCCATGAGAGACGGTGTCAGCAATGC TGCAGTGGTGAGTGCCAAGCTGAAACTCTTTGTGTTTGGAGGGACCAGCATCCACCGAGATATGGTGTCC AAAGTCCAGTGTTTTGACCCCTCGGAGAACCGGTGGACAATCAAAGCAGAGTGTCCCCAGCCCTGGCGAT ACACAGCCGCCGCTGTCCTGGGCAGCCAGATCTTCATCATGGGAGGTGATACAGAGTACACAGCAGCTTC AGCCTACCGCTTCGACTGTGAGACCAACCAGTGGACGCGGATTGGGGACATGACAGCCAAACGCATGTCC TGTCATGCCGTGGCTTCAGGCAACAAGCTGTATGTGGTGGGAGGCTACTTTGGGACCCAGAGGTGTAAGA CCCTGGACTGCTATGACCCTACTTCAGACACATGGAACTGCATCACCACTGTGCCCTACTCTCTCATTCC CACGGCCTTTGTTAGCACCTGGAAGCATCTGCCTGCATGAAAGGCACCTTTGGAGCCCAGCCAGGTGAGC CCTCAGGAGGGCTGGAACCCTCTGACTTATCTCTGCTCTTGGCAATGATTATCACATCTCCCAGGGAGGG TCTGATGAGCATCAGGGTAATGGGATTTAGGGACAGACGGTCTGTTCATATCTTACCAGTTATGGGTTTG TCAGTGGGAGAGGTAGTGTCTGTCTTCTGTGTGCAGTGGAGGGATGAAGGCCCATGTAAACCAGCAGCCA TTCTGACGAAGTGAGGTCTAATGAAGTGACGTGGCTGTTCTGATCCTCAGACGGGGCCTACCACAGGACA GGTGTCTGTGAGCCCCGCTGCTGTTGTCAGTGCTGTGAGTAAATAAAGTCATAAGAAGTCACAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_182782 |
| Insert Size | 1770 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC027373, AAH27373 |
| RefSeq Size | 2375 bp |
| RefSeq ORF | 1770 bp |
| Locus ID | 207952 |
| UniProt ID | Q8R2P1 |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for translational homeostasis. The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1): ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 3' UTR, compared to variant 1. Variants 1-3 encode the same the protein. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209189 | Klhl25 (tGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25) |
CN¥ 5,040.00 |
|
| MG216374 | Klhl25 (tGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25) transcript variant 3, (10ug) |
CN¥ 5,040.00 |
|
| MR216374 | Klhl25 (Myc-DDK-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 |
CN¥ 4,392.00 |
|
| MR216374L3 | Lenti ORF clone of Klhl25 (Myc-DDK-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 |
CN¥ 6,460.00 |
|
| MR216374L4 | Lenti ORF clone of Klhl25 (mGFP-tagged) - Mouse kelch-like 25 (Drosophila) (Klhl25), transcript variant 3 |
CN¥ 6,460.00 |
