Bcat1 (NM_007532) Mouse Untagged Clone
CAT#: MC202068
Bcat1 (untagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2, (10ug)
CNY 3,656.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | BCATc; Eca39 |
Vector | PCMV6-Kan/Neo |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC053706 sequence for NM_007532
AAGAGGTAATCCCGGACTGTAGCTCGCGGCTGAGCCAGTCGCTGCTGATGCAATCCGCAGCGGAGCGCGC CCAGCTCTCCATCTTCCGCGCTTGCCGACCGACCCTGAGCGCCACGTGTCACTGCACAGCCGCCTCCTCC AGAGGAGACTCACATTTGCACCCAGGAGCCGGAGCCACGACCCAAGCCACGAGAAATGAAGGACTGCAGT AATGGATGCTCCGCGCCGTTTGCTGGAGAAAGAGGATCAGAAGAAGTGGCGGAGACTTTTAGGGCCAAAG ATCTCATCATCACACCAGCCACTGTCTTAAAAGAGAAGCCGGACCCGGATTCGCTGGTCTTTGGAGCTAC GTTTACTGACCACATGCTGACGGTGGAGTGGTCCTCTGCGTCTGGATGGGAGAAACCTCACATTAAGCCT TTTGGAAACTTGCCCATACATCCCGCTGCCTCTGTTTTGCACTACGCTGTGGAACTGTTTGAAGGCTTGA AAGCCTTTCGGGGAGTTGATAACAAGATCCGATTGTTCCGGCCGGACCTCAACATGGATAGAATGTGCCG ATCTGCTGTGAGGACCACGCTGCCGATGTTTGACAAGGAGGAGCTCCTAAAGTGTATTCTTCAGCTTCTA CAGATCGACCAAGAATGGGTTCCCTACTCCACCTCTGCCAGCCTCTACATCCGCCCCACATTTATCGGAA CTGAGCCATCTCTTGGCGTCAAGAAGCCTTCCAAAGCCCTACTCTTTGTGATCCTGAGCCCCGTGGGACC TTATTTTTCTAGTGGATCTTTTACTCCGGTGTCCCTGTGGGCCAATCCAAAGTATATCAGAGCCTGGAAA GGTGGGACTGGAGACTGCAAGATGGGCGGCAATTATGGAGCCTCCCTTCTGGCACAGTGCGAGGCCGCGG AGAATGGCTGTCAGCAGGTCCTGTGGCTGTACGGCAAGGACAACCAGATAACTGAAGTAGGCACAATGAA TCTTTTCCCCTACTGGATAAACGAAGACGGAGAAGAGGAGCTGGCAACGCCCCCACTAGATGGCATCATT CTCCCAGGAGTGACCAGGCAGAGCATCCTGGAGCTGGCACAACAGTGGGGTGAGTTTAAGGTATGTGAGA GACACCTCACCATGGATGACCTGGCCACCGCCCTTGAGGGGAACAGAGTGAAGGAGATGTTCGGCTCAGG GACAGCCTGCGTTGTCTGCCCAGTCTCTGATATTCTGTACAAGGGCCAGATGCTGCATATTCCAACGATG GAGAATGGCCCCAAGCTTGCAAGTCGAATCCTGGGAAAGCTGACTGATATCCAGTATGGAAGGGTGGAGA GTGACTGGACAATCGAGCTACCCTGACGGCAAAGCTCAGGGTATCGGTGACGGTGCCACAGAAGAGCGAC TCCTGCCCTCGAGCTGGGACCACCTGTGACCATCTAGTCTTGCACATTGTAGTCGCGTTCCTCATGTGTT ACCAAATGAGGTTTTTTCCTTGAGCCACAGAAAGTTGCCAGTGTTCAATGCCACCTATATACATGGTAGC AATTGCTTACTGTAGTCTCCCTCACCCTGTACCAGATCCTTAGTAGGAGAATGGGTTCCCAAACCAATAA GATGTCTATGTGCTGGAAGGGCTACTGAGGAAATGTCACAGGCGGCCTCCCTGTGACTCATGGGCACTCT CAGAGCAGGACACTTTGTTTCTTTTGCTGGTGAGGTTCTTTTGTTTTTTGTTTTGGTTGTTTTTTTTTTG TTTGTTTGTTTCTAATTGGGGCAGATTTGGGCTCCCCATGCAGCCCTGCTGGCCTGGTGTTCCCTAATGT TCACCCCGGCTGGTCTCAGACTCAGAGCAGCCTTCCCGTCTCCCTCAGCCTTCTGGATGCTGGGGTGATT GACAGGCGTCCTACCATGATTTGTTTTTGTTTTTTAATCAAAAGTTCGGTCACCTGGATTTCTTTTCTCC GTTGGTCCCTGGGTCCACGCTAAGGTGACTGTCCCTTCTTCGTATCACGGTCTGCACTAATCTCACCCAA GGTGTCTGAGGCTGGTTGTCCGCACTGCCCTTTTTACGTAACTATAACTGGCTGCTCACTGCGCTGCTAT GAGACTAGGGGTCCTGTGCCTTCTAGATCTTTGCATCCTCAAAGCCCAGCACATAGTAGGTATTTTCAGT ACATGTTTACTCTAAGAATGAACATAGAAGTGTACTTTCTGTCTCTCCATGCTGAAACCCTTTCGGAAGC CAGGCCTGATTTGGGCAGCATGTAGCAGTATTTACCTCAGGCTGTCTTTCCTGTCCATGGACTCCTCCAG ACAGCACAGTCAGTTGGAAGAATTGTCTTATGTTTTAAGATTATCTTGGAATTGAAGCGATTGCCCTGGA AAGATGAAATACTAACCACATGGAAGTGACAGATACTATACATTATGTGTTAAATGCCAAATAAAGGGTC AGGTTGGGAGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG |
Restriction Sites | RsrII-NotI |
ACCN | NM_007532 |
Insert Size | 1161 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC053706, AAH53706 |
RefSeq Size | 2493 bp |
RefSeq ORF | 1161 bp |
Locus ID | 12035 |
UniProt ID | P24288 |
Gene Summary | Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5'-terminal exon, compared to variant 1. The resulting protein (isoform 2) has a shorter and unique N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR206032 | Bcat1 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2 |
CNY 3,656.00 |
|
MR206032L3 | Lenti ORF clone of Bcat1 (Myc-DDK-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2 |
CNY 6,056.00 |
|
MR206032L4 | Lenti ORF clone of Bcat1 (mGFP-tagged) - Mouse branched chain aminotransferase 1, cytosolic (Bcat1), transcript variant 2 |
CNY 4,750.00 |