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Xrcc5 (NM_009533) Mouse Untagged Clone
CAT＃: MC202035
Xrcc5 (untagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 5 (Xrcc5), (10ug)
CNY 5,368.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI314015; CTC85; CTCBF; Ku80; Ku86 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC051660 sequence for NM_009533
CGGAACTGAGTTGCGACACTCTAGGTTTCCGCCCGGAAAAAGTAATCAAATCACCTGAGGACCAGCATGG CGTGGTCCGGTAATAAGGCAGCTGTTGTGCTGTGTGTGGATGTGGGGGTTGCCATGGGTAACTCCTTTCC TGGTGAAGAATCTCCAATTGAACAAGCAAAGAAAGTGATGACTATGTTTGTCCAACGACAGGTATTTTCG GAGAGCAAAGATGAGATTGCGTTAGTCCTCTATGGCACAGATGGCACTGATAATGCCCTTGCTGGCAAGG ACCAGTATCAGAACATCACAGTGTGCAGACACCTGATGCTACCAGATTTTGATTTGCTGGAAGACATCGG AAACAAAATCCAGCCAAGTTCCCAGCAAGCCGACTTCCTGGACGCCCTGATTGTGTGCATGGATTTGATT CAGCGTGAAACCATAGGGAAGAAGTTTGGGAAGAAGCATATTGAAGTGTTCACTGACCTCAGCAGCCCGT TCAGCCAGGACCAACTGGACGTTATAATTTGTAACTTGAAGAAGTCTGGCATCTCTCTGCAGTTCTTTTT GCCTTTTCCAATCGACAAGAATGGCGAGCCTGGCGAGAGAGGGGACCTGGACTCTGGCTTGGACCACCTC AAACCCTCCTTCCCTCAAAAAGGACTTACTGAGCAGCAAAAGGAAGGCATCCGCATGGTGACAAGGGTGA TGCTGTCCTTAGAAGGCGAAGACGGGCTGGATGAAATTTATTCCTTCAGTGAGAGTCTACGACAACTGTG CGTCTTTAAGAAGATTGAGAGGCGTTCCATGCCCTGGCCCTGCCAACTGACCATTGGCCCCAACTTGTCT ATAAAGATTGTAGCCTATAAATCGATTGTACAGGAGAAATTTAAGAAGAGTTGGGTAGTTGTGGACGCAA GAACGCTAAAGAAGGAAGATATACAAAAAGAGACTGTTTATTGCCTAAATGACGATGATGAAACTGAAGT TTCCAAAGAGGACACTATTCAAGGGTACCGCTACGGAAGTGATATCATTCCTTTTTCTAAAGTGGATGAG GAACAAATGAAATATAAATCTGAGGGGAAGTGCTTCTCTGTTTTGGGATTTTGTAAATCTTCTCAGGTTC ATAGAAGATTCTTCATGGGACATCAAGTTCTAAAGGTCTTTGCAGCAAAGGATGATGAGGCGGCAGCTGT TGCCCTTTCCTCTCTCGTTCATGCTTTGGATGAGTTAAACATGGTCGCCATCGTCCGATATGCTTATGAC AAAAGATCTAATCCTCAAGTTGGTGTAGCCTTCCCTTACATTAAGGACGCCTATGAGTGTTTAGTTTATG TGCAGCTGCCTTTCATGGAAGACTTGCGGCAATACATGTTTTCCTCGCTGAAAAACAATAAGAAGTGCAC TCCCACAGAGGCCCAGCTGAGCGCTATTGATGATCTGATTGATTCCATGAGCTTGGTAAAGAAAAACGAG GAAGAAGACATTGTTGAAGATTTGTTTCCAACCTCCAAAATTCCAAATCCTGAATTTCAGCGATTGTACC AGTGTCTGCTGCATAGAGCCTTACATCTCCAGGAGCGGCTGCCCCCGATTCAGCAGCACATTTTGAATAT GCTGGATCCCCCCACTGAGATGAAAGCAAAATGTGAGAGTCCACTCTCTAAAGTAAAGACCCTTTTCCCT CTCACAGAAGTCATCAAGAAAAAGAACCAAGTGACTGCTCAGGACGTTTTCCAAGACAATCATGAAGAGG GGCCCGCTGCTAAAAAATATAAAACTGAGAAAGAAGAAGATCACATCAGCATCTCCAGCCTGGCAGAAGG GAACATCACCAAGGTTGGAAGTGTGAATCCTGTTGAAAACTTCCGTTTCCTAGTAAGACAGAAGATTGCC AGCTTTGAGGAAGCGAGTCTCCAGTTAATAAGTCACATCGAACAATTTTTGGATACCAATGAAACACTGT ATTTTATGAAAAGTATGGACTGCATCAAAGCTTTCCGGGAGGAGGCCATTCAGTTTTCAGAAGAACAGCG CTTCAACAGCTTCCTGGAAGCCCTTCGAGAGAAAGTGGAAATTAAGCAATTAAATCATTTCTGGGAAATT GTTGTTCAGGATGGAGTTACTCTGATCACCAAAGACGAAGGCCCAGGAAGCTCTATCACAGCTGAGGAAG CCACAAAGTTTCTGGCCCCCAAAGACAAAGCAAAAGAAGATACAACGGGACCTGAAGAAGCTGGTGATGT GGATGATTTACTGGACATGATATAGATCATGGATGTGTGGGGAATCCAAGAGTGCCAGCTCTGTGCTGCT GAGAGCTCTGGCAGCATCCCTGAGGCCGTGCAGGGGATGTGAAGCTCTGGACGTCATCCTAGCAGACAAC TTGGGAATGAGTCACTCCAGGTCTCTACAGAGTGACCATAAGGACATATATTACAAGAAATAACTTAGAC CCTATTCAAATTTATAAAGAGCCATTATTTTCTGAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_009533 |
| Insert Size | 2199 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC051660, AAH51660 |
| RefSeq Size | 2500 bp |
| RefSeq ORF | 2199 bp |
| Locus ID | 22596 |
| UniProt ID | P27641 |
| Gene Summary | Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG210341 | Xrcc5 (tGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 5 (Xrcc5) |
CNY 7,130.00 |
|
| MR210341 | Xrcc5 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 5 (Xrcc5) |
CNY 5,360.00 |
|
| MR210341L3 | Lenti ORF clone of Xrcc5 (Myc-DDK-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 5 (Xrcc5) |
CNY 8,360.00 |
|
| MR210341L4 | Lenti ORF clone of Xrcc5 (mGFP-tagged) - Mouse X-ray repair complementing defective repair in Chinese hamster cells 5 (Xrcc5) |
CNY 8,360.00 |
