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Hmces (NM_173737) Mouse Untagged Clone
CAT＃: MC201841
8430410A17Rik (untagged) - Mouse RIKEN cDNA 8430410A17 gene (8430410A17Rik), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 8430410A17Rik; C85376 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC024401 sequence for NM_173737
CTAGCTGCGAGGAAGGGTGGACTAGACCCTCGGGCGGTCTGAGCGGCAGCGCTGAAGTATGTGCGGGCGA ACGTCCTGTCACTTGCCCAGAGAGGTTCTCACCAGGGCCTGCGCCTATCAGGATCGGCAGGGCCGGCGGC GGCTCCCGCAGTGGAGGGACCCCGACAAGTACTGCCCCTCCTACAACAAGAGCCCGCAGTCCAGCAGCCC CGTGCTGCTCTCCAGACTGCACTTTGAGAAGGATGCAGACTCATCAGATCGGATAATTATTCCCATGCGA TGGGGCTTAGTCCCATCTTGGTTCAAAGAAAGTGATCCTTCTAAGCTGCAGTTCAACACTACCAACTGTC GTAGTGATACCATAATGGAGAAGCAGTCATTCAAGGTTCCTCTGGGGAAAGGACGGCGGTGTGTTGTTTT AGCAGATGGATTCTACGAGTGGCAGCGGTGTCAGGGAACAAACCAGAGGCAACCATACTTCATCTATTTT CCTCAAATCAAGACAGAGAAGTCAGGTGGGAACGATGCTTCAGACAGCTCTGACAACAAGGAAAAGGTCT GGGACAACTGGAGGCTGCTGACAATGGCAGGGATCTTTGACTGCTGGGAAGCGCCAGGGGGAGAGTGCCT GTATTCCTACAGCATCATCACTGTGGATTCCTGCAGAGGTTTGAGTGACATCCACAGCAGGATGCCTGCC ATACTAGATGGAGAAGAAGCAGTCTCCAAATGGCTCGACTTTGGTGAGGTCGCCACTCAGGAAGCTCTGA AGCTAATCCACCCCATAGACAATATCACCTTCCATCCAGTTTCTCCAGTGGTGAACAATTCCCGAAACAA CACTCCGGAGTGTCTGGCGCCTGCTGACTTGCTGGTTAAGAAGGAGCCCAAGGCAAATGGCAGCAGTCAA AGGATGATGCAGTGGCTGGCTACAAAGTCACCCAAAAAGGAAGTCCCTGACTCACCCAAAAAGGATGCAT CAGGTCTACCCCAGTGGTCCAGCCAGTTTCTCCAGAAGAGCCCATTGCCTGCTAAAAGAGGTGCTACCAG CAGCTTCCTGGATCGATGGCTGAAGCAGGAGAAGGAGGATGAGCCCATGGCCAAGAAGCCTAACAGCTAG GCTCCATACCAGGCCTCAGCCTGATACAGCATTACTGTTGTCACCAGTTGACTGGAATTGTAACCTTTTA CTTGGGAATGTGGTGGCCCAATGTCAGCTGACCTGAAAGAACATGGCTACCGTGAGACAGCCCTGTGGGC CCTGCTACCCCTCCTATTTCTGCCTCCCTGCTGGGAGCTTTCCTGTTCCCTGGTGTTCCACGAAGGAGGC TCTTCTCAGTCTCTGGGCTCTCCTTTAGAGTTAAGGTCTTAAAATGTATTTTGTCCTAAATAAACTGCGT TTGGTAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_173737 |
| Insert Size | 1062 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | BC024401, AAH24401 |
| RefSeq Size | 1422 bp |
| RefSeq ORF | 1062 bp |
| Locus ID | 232210 |
| UniProt ID | Q8R1M0 |
| Gene Summary | Sensor of abasic sites in single-stranded DNA (ssDNA) required to preserve genome integrity by promoting error-free repair of abasic sites (By similarity). Acts as an enzyme that recognizes and binds abasic sites in ssDNA at replication forks and chemically modifies the lesion by forming a covalent cross-link with DNA (By similarity). The HMCES DNA-protein cross-link is then degraded by the proteasome (By similarity). Promotes error-free repair of abasic sites by acting as a 'suicide' enzyme that is degraded, thereby protecting abasic sites from translesion synthesis (TLS) polymerases and endonucleases that are error-prone and would generate mutations and double-strand breaks (By similarity). Acts as a protease: mediates autocatalytic processing of its N-terminal methionine in order to expose the catalytic cysteine (PubMed:29020633). Specifically binds 5-hydroxymethylcytosine (5hmC)-containing DNA in stem cells (PubMed:23434322). May act as an endonuclease that specifically cleaves 5hmC-containing DNA; additional experiments are however required to confirm this activity in vivo (PubMed:29020633).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205366 | 8430410A17Rik (tGFP-tagged) - Mouse RIKEN cDNA 8430410A17 gene (8430410A17Rik) |
CNY 3,140.00 |
|
| MR205366 | 8430410A17Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 8430410A17 gene (8430410A17Rik) |
CNY 3,656.00 |
|
| MR205366L3 | Lenti ORF clone of 8430410A17Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 8430410A17 gene (8430410A17Rik) |
CNY 4,750.00 |
|
| MR205366L4 | Lenti ORF clone of 8430410A17Rik (mGFP-tagged) - Mouse RIKEN cDNA 8430410A17 gene (8430410A17Rik) |
CNY 4,750.00 |
