Ptger2 (BC005440) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	EP2; Ptgerep2
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC005440 CCTGCCTGGCCCCACGGCAGCAGCTCGCAAGCAATCTAAATTGCTTTCCTGGATTCCACTACTGGACTTG CCCCCTGAAGGCGCTGGAGGGAGCAGCTGCTCTGGCAAGCACCCCCTGCTAGGGCAGGTGAGGCACAGAA GCACCGAGAGCGACCGGATATTGTAGTGAAGAGGCCACTGTACGTACAGGCAGGAGACCCAAACAAGTCT GTCCTTGGTGCGAGTTGGGGGCCGGAAGGGAGCTCTGGATTTCGGTCCCTCCCCTTTTCCCTGCTCTGTC TTGGAGCCCTGGGGCCATCAGACCCTCCGACTGTCTGGTACTTGCCTGGAAGAGATATCATCTCTCCTCC ACACCCTCCACCATGGACAATTTTCTTAATGACTCCAAGCTAATGGAGGACTGCAAGAGTCGTCAGTGGC TCCTTTCGGGGGAAAGCCCAGCCATCAGCTCGGTGATGTTCTCGGCCGGGGTTCTGGGGAATCTCATCGC ACTGGCACTGTTGGCGCGCCGCTGGCGTGGGGACACCGGGTGTAGCGCCGGCAGCAGGACCTCTATCTCC TTGTTTCACGTGCTGGTAACGGAATTGGTGCTCACTGACCTGCTGGGAACCTGCCTCATCAGCCCGGTGG TGCTGGCTTCATATTCAAGAAACCAGACCCTGGTGGCCCTGGCTCCCGAAAGTCACGCGTGTACCTATTT CGCTTTCACTATGACCTTCTTTAGTCTGGCCACGATGCTCATGCTCTTCGCTATGGCCCTGGAACGCTAC CTCTCCATCGGGTACCCTTACTTCTACAGGCGCCACTTATCGCGCCGCGGGGGTCTGGCGGTGCTGCCTG TCATCTATGGGGCCTCCTTGCTCTTCTGTTCCCTGCCGCTGCTCAACTACGGGGAGTACGTCCAGTACTG TCCTGGGACATGGTGCTTTATCCGGCACGGGAGGACTGCATACCTTCAGCTGTACGCCACGATGCTCCTG CTGCTTATCGTGGCTGTGCTCGCCTGCAACATCAGCGTTATCCTCAACCTCATTCGCATGCACCGTCGGA GCAGAAGAAGCCGCTGCGGATTGTCTGGCAGTAGCCTAAGAGGCCCTGGATCTCGCAGGAGAGGAGAGAG GACTTCGATGGCAGAGGAGACGGACCACCTCATTCTCCTGGCCATTATGACCATCACCTTCGCCATATGC TCCTTGCCTTTCACAATCTTTGCCTACATGGATGAAACCTCTTCCCTAAAGGAAAAGTGGGACCTCCGAG CTCTTCGGTTTTTATCAGTTAACTCTATAATTGACCCTTGGGTCTTTGCCATACTTAGGCCACCGGTCCT GAGGTTAATGCGCTCAGTCCTCTGTTGTCGGACTTCACTGAGAACACAAGAAGCTCAGCAAACATCCTGT TCTACCCAGTCCAGTGCCAGTAAACAGACTGACCTTTGTGGACAGTTGTGAGGATGCGCTTCATGAGGGA ACCTCTGAAGAAGTCTTTAAATGGCCTCATTGGAGAAGTGTAAGGGGCTGGAATATAAACAGAATAACCT TGCCCTGAGAAGCAGATGAAACAGACTTTATGAGGTAGTTTGGGCTGCAGATGTGATGACAGAGCCCTTT GTGGAAAGTGTCAGAGGATATAAAGTTCACATTATGTGACCTTTGAAGGACAATCGGCTGCGTCTAAGAC CCAGTTGATTTAAGCTTTCCTGTTGAGTGACAAAGCATGTGGTTTTGTAATTTGTTTTAAAATGCTACAT GGTTACTGTTTTAATTTTGATGTCACTGCTGTTATAAATGCAATCAGACCAAATGAAATTCATTCTCTAG GAAGTCAACTCATGCAAGCGATGAAGCAACCAGAGCAGACAAGTTGCTGTCTCGTGATTGCTTAATGGAC TCTTTATTTGGACCATGAAGTTGAATGCCATTGGCCCCTTTTACTGTGCGCGTTCATGTGGGAGTCCTCT CTGGTAACGAAAGGATGCACTGATTTGGTTAATGCTAGTTAATCCTTTAGGAGCTCTGTCATGTTGTCAG ATTATTTATTCTGTCTTGTCTATTGTGATAAGGCTACTGAAGAAAACTTCAGAAATTTGCGTCTCACAAA GGAATAATTAAAAAAATCAATTTTAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC005440
Insert Size	1089 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC005440, AAH05440
RefSeq Size	2139 bp
RefSeq ORF	1089 bp
Locus ID	19217
Gene Summary	Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.[UniProtKB/Swiss-Prot Function]

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