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Gba (NM_008094) Mouse Untagged Clone
CAT＃: MC200608
Gba (untagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 1, (10ug)
CNY 3,840.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | betaGC; GBA1; GC; GCase; GLUC |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC200608 representing NM_008094.
Blue=ORF Red=Cloning site Green=Tag(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCG CGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGAC ATGGCTGCCAGGCTCATCGGATTCTTCCTATTTCAGGCGGTATCTTGGGCATATGGTGCCCAACCCTGC ATCCCCAAAAGCTTTGGCTACAGCTCAGTGGTCTGTGTCTGTAATGCATCGTACTGTGACTCTCTTGAC CCCGTGACCTTACCGGCTCTGGGTACCTTCAGCCGTTACGAGAGCACTCGACGTGGACGTCGGATGGAG CTGAGTGTCGGGGCCATCCAGGCCAATCGCACTGGCACAGGGTTACTACTCACTTTGCAGCCAGAAAAG AAGTTCCAGAAAGTGAAAGGATTTGGAGGCGCCATGACAGACGCCACTGCGCTCAACATCCTTGCTTTG TCCCCACCTACTCAGAAGCTGCTACTCAGATCCTACTTCTCTACCAACGGAATTGAATATAACATCATC CGGGTACCCATGGCCAGTTGTGACTTCTCCATCCGTGTCTATACCTATGCTGACACCCCTAACGACTTC CAGTTATCCAACTTCAGCCTCCCAGAAGAAGACACCAAGCTCAAGATACCCCTGATTCACCAAGCCCTG AAGATGTCCTCACGCCCCATTTCACTCTTTGCCAGTCCCTGGACATCACCCACTTGGCTCAAGACCAAT GGAAGAGTGAATGGGAAGGGGTCGCTCAAGGGTCAGCCAGGGGATATCTTTCACCAGACCTGGGCCAAT TACTTTGTCAAGTTTCTGGATGCTTATGCTAAGTATGGCCTAAGATTCTGGGCAGTGACAGCGGAGAAT GAACCTACGGCAGGGCTCTTCACGGGGTACCCCTTCCAGTGCCTGGGCTTCACTCCTGAACACCAGAGA GACTTCATTTCCCGTGACCTAGGGCCAGCCCTTGCCAACAGTTCCCATGATGTGAAGCTACTCATGCTA GATGACCAACGCTTGCTGCTACCCCGCTGGGCAGAGGTGGTGCTCTCTGATCCAGAGGCAGCTAAGTAC GTTCATGGCATTGCTGTTCACTGGTATATGGATTTCCTGGCTCCAGCCAAAGCTACTTTAGGAGAGACA CACCGCTTGTTCCCCAACACAATGCTCTTTGCTTCGGAGGCCTGCGTGGGCTCCAAGTTCTGGGAACAG AGTGTTCGGTTGGGCTCCTGGGATCGAGGGATGCAGTACAGTCACAGCATCATTACGAACCTCCTTTAC CACGTAACTGGATGGACGGACTGGAACCTTGCCCTGAATCCTGAAGGAGGGCCCAACTGGGTCCGCAAC TTTGTCGATAGCCCCATTATTGTCGACATCCCCAAAGACGCATTTTACAAACAGCCCATGTTCTACCAT CTTGGCCACTTCAGCAAGTTCATTCCCGAGGGATCCCAGAGAGTGGCGTTGGTGGCCAGTGAGAGCACT GACTTGGAAACAGTAGCACTGTTACGCCCTGACGGCTCTGCAGTTGTGGTCGTGTTAAACCGATCTTCG GAGGATGTCCCTCTTACCATCAGTGATCCTGACCTGGGCTTCCTGGAGACCGTGTCACCTGGCTACTCC ATTCACACTTACCTGTGGCGTCGCCAGTGA ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAG GATGACGACGATAAGGTTTAAACGGCCGGCCGCGGT |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_008094 |
| Insert Size | 1548 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC006663 |
| RefSeq Size | 2238 bp |
| RefSeq ORF | 1548 bp |
| Locus ID | 14466 |
| UniProt ID | P17439 |
| MW | 57.6 kDa |
| Gene Summary | Glucosylceramidase that catalyzes, within the lysosomal compartment, the hydrolysis of glucosylceramide/GlcCer into free ceramide and glucose (PubMed:24211208). Thereby, plays a central role in the degradation of complex lipids and the turnover of cellular membranes (PubMed:27378698). Through the production of ceramides, participates to the PKC-activated salvage pathway of ceramide formation (By similarity). Also plays a role in cholesterol metabolism (PubMed:24211208). May either catalyze the glucosylation of cholesterol, through a transglucosylation reaction that transfers glucose from glucosylceramide to cholesterol (PubMed:24211208). The short chain saturated C8:0-GlcCer and the mono-unsaturated C18:0-GlcCer being the most effective glucose donors for that transglucosylation reaction (By similarity). Under specific conditions, may alternatively catalyze the reverse reaction, transferring glucose from cholesteryl-beta-D-glucoside to ceramide (By similarity). Finally, may also hydrolyze cholesteryl-beta-D-glucoside to produce D-glucose and cholesterol (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Gut-seeded α-synuclein fibrils promote gut dysfunction and brain pathology specifically in aged mice
,null,
Nature neuroscience
,PubMed ID 32066981
[Gba]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG208259 | Gba (tGFP-tagged) - Mouse glucosidase, beta, acid (Gba) |
CNY 5,040.00 |
|
| MR208259 | Gba (Myc-DDK-tagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 1 |
CNY 3,840.00 |
|
| MR208259L3 | Lenti ORF clone of Gba (Myc-DDK-tagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 1 |
CNY 6,460.00 |
|
| MR208259L4 | Lenti ORF clone of Gba (mGFP-tagged) - Mouse glucosidase, beta, acid (Gba), transcript variant 1 |
CNY 6,240.00 |
