P4hb (NM_011032) Mouse Untagged Clone

CAT#: MC200577

P4hb (untagged) - Mouse prolyl 4-hydroxylase, beta polypeptide (P4hb), (10ug)



  "NM_011032" in other vectors (4)

CNY 3,800.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
P4HB Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms ERp59; PDI; Pdia1; Thbp
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC008549 sequence for NM_011032
CTTGACTGTTCCGGCGGCGCCAACCCAACTGCCCCGCCCGCTGCAGACGTCCGACATGCTGAGCCGTGCT TTGCTGTGCCTAGCCCTGGCCTGGGCGGCTAGGGTGGGCGCCGACGCCCTGGAGGAGGAGGACAACGTCC TGGTGTTGAAGAAGAGCAACTTCGAGGAGGCGCTGGCGGCGCACAAGTACCTGCTGGTGGAGTTCTATGC CCCGTGGTGTGGCCACTGCAAAGCTCTGGCTCCCGAGTATGCCAAAGCTGCCGCAAAACTGAAGGCAGAA GGCTCTGAGATTCGACTAGCAAAGGTGGATGCCACAGAAGAGTCTGACCTGGCTCAGCAGTATGGTGTCC GTGGCTACCCCACAATCAAGTTCTTCAAGAATGGAGACACAGCCTCCCCAAAGGAATATACAGCTGGCAG GGAAGCTGATGACATTGTCAACTGGCTGAAGAAACGCACGGGCCCAGCAGCTACAACCCTGTCTGACACT GCAGCTGCAGAGTCCTTGGTGGACTCAAGCGAAGTGACGGTCATCGGTTTCTTCAAGGACGTAGAGTCAG ACTCTGCCAAGCAGTTCTTGCTGGCAGCAGAGGCTATTGATGACATACCTTTTGGAATCACGTCCAACAG TGGTGTGTTCTCCAAGTACCAGCTGGACAAAGATGGGGTGGTCCTCTTTAAGAAGTTTGATGAAGGCCGC AACAACTTTGAGGGTGAGATCACCAAGGAGAAGCTTCTAGACTTCATCAAGCACAACCAGCTGCCTTTGG TCATCGAGTTCACTGAACAGACAGCTCCGAAGATTTTTGGAGGTGAGATCAAGACACACATTCTGCTGTT CCTGCCCAAGAGTGTATCTGACTATGACGGCAAGCTGAGCAGCTTCAAGAGAGCAGCCGAGGGCTTCAAG GGCAAGATCCTATTTATCTTCATCGATAGCGACCACACTGATAACCAGCGCATACTTGAGTTCTTTGGCC TGAAGAAGGAGGAGTGTCCCGCCGTGCGGCTTATTACCCTAGAGGAAGAGATGACCAAGTACAAACCAGA GTCAGATGAGCTGACGGCTGAGAAAATCACAGAGTTTTGCCACCGCTTCTTAGAGGGCAAGATCAAGCCC CACCTGATGAGCCAGGAAGTGCCTGAAGACTGGGACAAACAGCCGGTAAAAGTACTGGTTGGGGCGAACT TTGAAGAGGTCGCTTTTGATGAGAAAAAGAACGTGTTTGTTGAATTCTATGCCCCTTGGTGTGGTCACTG CAAGCAGCTAGCCCCCATTTGGGATAAACTGGGAGAGACATACAAGGATCATGAGAATATCATCATTGCT AAGATGGACTCAACAGCCAATGAGGTGGAAGCTGTCAAAGTACATAGCTTTCCCACACTGAAGTTCTTCC CAGCAAGTGCAGACAGAACGGTCATTGATTACAATGGCGAGCGGACACTGGACGGTTTTAAGAAATTCTT GGAGAGTGGTGGCCAGGATGGAGCAGGGGACGATGAGGACCTCGACCTAGAAGAAGCTTTAGAGCCAGAC ATGGAGGAAGACGACGATCAGAAAGCTGTGAAGGATGAACTGTAGTGCAGAAGCCAGATCTGGGCGCCTG AACCCAAAACCTCGGTGGGCCACGCTCCCAGCAGCCCACATCTCCGGAGCCTGAACCTCACCCCAGGAGG GAGCGCCATCAGAACCCAGGGAATCTTTCTGAAGCCACACTCATCTGACACACGTACACTTAGACCTGTC TCTTCTTTTGCTTTTCAATTTTGGAAAGGGATCTCTGTCCAGGTCAGCCCCTCTTGAAGGGCTACCTTTT GTTTTAATTGGTGGTGTACTTTTTTGTATGTGGAATTTGTCCCATGTGCTTGCTAAAATGTTTGGGGATC TCCTCACACTGGTAATGTCTTTCCTGTTAGAGAGGTTTATGCTACCACTTCGCATTTCATCTGTGAGGCA TTTCATCTTTTAGACATGTCTTCATGTCCAGCTACTTGTTTCACCATGGCAGACCTCCCTGAGCCTTCTT CCTGCCCTGGGTAGGAAGTGTGGCTCTGGGTCTGTGGTCACTCTCCACCTTGTCCTAGTGTTGCCATGGT AGCATGGCTTTGTAGTTTGCATTTAACCTGGGGATCTATGCATCCTGTCAAGAGGGTGGGTCCCCGTCCC CGTGTGTGGAAAAGAGACAGTGTGGCTTGCTGCCAGGCTCAGGCCAGGTCTGGACAGCTCTCACTCTCCT CAAGCCAGGACTACCGACCAGCCGGCTATGGGCACATTACTCTGTGGCTGCTGGATTCTTTTCCAGCATG GCCTGTGGCCTGTGTGAGGCAGAACTGGGACCCTTGATTCCCAGATTGGGAGTCAGCCAAGGACACTGGG GCTGAATGAAATGCCCATTCTTCAGGCATTTCTACCATAATATTGGAATTGAACACATTGGCTAAATAAA GTTGAAATTTTAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_011032
Insert Size 1530 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008549, AAH08549
RefSeq Size 2476 bp
RefSeq ORF 1530 bp
Locus ID 18453
UniProt ID P09103
Gene Summary This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP (By similarity). Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration (PubMed:21670307).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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