Atp5d (NM_025313) Mouse Untagged Clone

CAT#: MC200271

Atp5d (untagged) - Mouse ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (Atp5d), nuclear gene encoding mitochondrial protein, (10ug)



  "NM_025313" in other vectors (4)

CNY 2,400.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 0610008F14Rik; 1500000I11Rik; AA960090; AI876556; AU020773; C85518
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC008273 sequence for NM_025313
CTCGCTTGCCCAGTGTGTCGGCCGCCCGCGAAGCTAGAGTCCACTGACTTTTCCGCCACCATGTTGCCCG CCTCACTGCTTCGTCACCCGGGCCTGCGCCGCCTGATGCTTCAGGCGCGTACATACGCCGAGGCCGCCGC TGCACCTGCCCCCGCCGCCGGGCCCGGACAGATGTCCTTCACCTTTGCCTCCCCGACGCAGGTGTTCTTT GACAGTGCCAACGTCAAGCAAGTGGACGTGCCTACGCTGACTGGAGCCTTTGGCATCTTGGCATCCCATG TCCCCACACTACAGGTCCTACGGCCTGGGCTGGTAGTGGTTCACACAGAAGACGGCACCACGACTAAGTA CTTTGTGAGCAGCGGCTCCGTCACTGTGAATGCCGACTCCTCTGTGCAGTTACTAGCTGAAGAAGCTGTG ACACTGGACATGCTGGACCTGGGGGCAGCCCGGGCCAACCTGGAGAAGGCGCAGTCAGAACTGTCAGGTG CGGCGGACGAGGCAGCACGGGCTGAGATCCAGATCCGTATTGAGGCCAATGAAGCCCTAGTGAAGGCCCT GGAGTAGGTGGTACCTACTGTCTGACACCCACGGGGAAACTGAGCCAGGTCCAGGCCGATGAGAAGTTCC CAGTGGGCTGAAGTGGCCACCAGGGGTCAGCAGTGCTCCAGTTGCTGGGCTTAAAGCTTCCTGGTGCCTG TCTGCCAGGTCATGGAGGATTCCCCAATCTGGCATCCCCACGATGCCTCTGGAGAGATGGCCTTGATTGC CCCTCAAAGCCACCTGAACCGTCGTCAACTTACCCAGCCTGTCTCCATTAAACACCAGGAACCAAAAAAA AAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN NM_025313
Insert Size 507 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC008273, AAH08273
RefSeq Size 849 bp
RefSeq ORF 507 bp
Locus ID 66043
UniProt ID Q9D3D9
Gene Summary Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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