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Nampt (NM_021524) Mouse Untagged Clone
CAT＃: MC200248
Nampt (untagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 1110035O14Rik; AI314458; AI480535; NAmPRTase; Pbef; Pbef1; Visfatin |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC004059 sequence for NM_021524
GCGCCGGGCAGCTCGTGGCGCGGCGTCTCCGCTCCGGCCCGAGATGAATGCTGCGGCAGAAGCCGAGTTC AACATCCTGCTGGCCACCGACTCGTACAAGGTTACTCACTATAAACAATACCCACCCAACACAAGCAAAG TTTATTCCTACTTTGAATGCCGTGAAAAGAAGACAGAAAACTCCAAAGTAAGGAAGGTGAAATACGAGGA AACAGTATTTTATGGGTTGCAGTACATTCTTAATAAGTACTTAAAAGGTAAAGTAGTGACCAAAGAGAAA ATCCAGGAGGCCAAAGAAGTGTACAGAGAACATTTCCAAGATGATGTCTTTAACGAAAGAGGATGGAACT ACATCCTTGAGAAATACGATGGTCATCTCCCAATTGAAGTAAAGGCTGTTCCCGAGGGCTCTGTCATCCC CAGAGGGAACGTGCTGTTCACAGTGGAAAACACAGACCCAGAGTGCTACTGGCTTACCAATTGGATTGAG ACTATTCTTGTTCAGTCCTGGTATCCAATTACAGTGGCCACAAATTCCAGAGAACAGAAGAAAATACTGG CCAAATATTTGTTAGAAACCTCTGGTAACTTAGATGGTCTGGAATACAAGTTACATGACTTTGGTTACAG AGGAGTCTCTTCGCAAGAGACTGCTGGCATAGGGGCATCTGCTCATTTGGTTAACTTTAAAGGAACAGAT ACTGTGGCGGGAATTGCTCTAATTAAAAAATACTATGGGACAAAAGATCCTGTTCCAGGCTATTCTGTTC CAGCAGCAGAGCACAGTACCATAACGGCTTGGGGGAAAGACCATGAGAAAGATGCTTTTGAACACATAGT AACACAGTTCTCATCAGTGCCTGTGTCTGTGGTCAGCGATAGCTATGACATTTATAATGCGTGTGAGAAA ATATGGGGTGAAGACCTGAGACATCTGATAGTATCGAGAAGTACAGAGGCACCACTAATCATCAGACCTG ACTCTGGAAATCCTCTTGACACTGTATTGAAGGTCTTAGATATTTTAGGCAAGAAGTTTCCTGTTACTGA GAACTCAAAAGGCTACAAGTTGCTGCCACCTTATCTTAGAGTCATTCAAGGAGATGGCGTGGATATCAAT ACTTTACAAGAGATTGTAGAGGGAATGAAACAAAAGAAGTGGAGTATCGAGAATGTCTCCTTCGGTTCTG GTGGCGCTTTGCTACAGAAGTTAACCCGAGACCTCTTGAATTGCTCCTTCAAGTGCAGCTATGTTGTAAC CAATGGCCTTGGGGTTAATGTGTTTAAGGACCCAGTTGCTGATCCCAACAAAAGGTCAAAAAAGGGCCGG TTATCTTTACATAGGACACCAGCGGGGAACTTTGTTACACTTGAAGAAGGAAAAGGAGACCTTGAGGAAT ATGGCCATGATCTTCTCCATACGGTTTTCAAGAATGGGAAGGTGACAAAAAGCTACTCATTTGATGAAGT CAGAAAAAATGCACAGCTGAACATCGAGCAGGACGTGGCACCTCATTAGGCTTCATGTGGCCGGGTTGTT ATGTGTGCAGTGTGTGTATACATACATGCACGTATGTGTGCGCCTGTGCGTATGTACTAACATGTTCATT GTACAGATGTGTGGGTTCGTGTTTATGATACACTGCAGCCAGATTATTTGTTGGTTTATGGACATACTGC CCTTTTTATTTTTCTCCCAGTGTTTAGATGATCTCAGATTAGAAAACACTTACAACCATGTACAAGATTA ATGCTGAAGCAAGCTTTTCAGGGTCCTTTGCTAATAGATAGTAATCCAATCTGGTGTTGATCTTTTCACA AATAACAAACCAAGAAACTTTTATATATAACTACAGATCACATAAAACAGATTTGCATAAAATTACCATG CCTGCTTTATGTTTATATTTAACTTGTATTTTTGTACAAACGAGATTGTGTAAGATATATTTAAAGTTTC AGTGATTTACCAGTCTGTTTCCAACTTTTCATGATTTTTATGAGCACAGACTTTCAAGAAAATACTTGAA ATAAATTACATTGCCTTTTGTCCATTAATCAGCAAATAAAACATGGCCTTAACCAAGTTGTTTGTGGTGT TGTACATTTGCAAATTATGTCAGGACAGACAGATACCCAACAGAGTTCCGAACATCACTGCCCTTGTAGA GTATGCAGCAGTCATTCTCCGTGGAAGAGAAGAATGGTTCTTACGCGAATGTTTAGGCATTGTACAATTC TGTGCCCTGGTCAGTGTATATGTACCAGTGATGCCAAATCCCAAAGGCCTGTTCTGCAATTTTATATGTC GGATATTGCCTGTGGCTCTAATATGCACCTCAAGATTTTAAGAAGATAATGTTTTTAGAGAGAATTTCTG CTTCCACTATAGAATATATACATAAATGTAAAATATTGAAAGTGGAAGTAGTGTATTTTAAAGTAATTAC ACTTCTGAATTTATTTTTCATATTCTATAGTTGGTATGTCTTAAATGAATTGCTGGAGTGGGTAGTGAGT GTACTTATTTTAAATGTTTTGATTCTGTTATATTTTCATTAAGTTTTTTAAAAATTAAATTGGATATTAA ACTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_021524 |
| Insert Size | 1476 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC004059, AAH04059 |
| RefSeq Size | 2633 bp |
| RefSeq ORF | 1476 bp |
| Locus ID | 59027 |
| UniProt ID | Q99KQ4 |
| Gene Summary | The secreted form behaves both as a cytokine with immunomodulating properties and an adipokine with anti-diabetic properties, it has no enzymatic activity, partly because of lack of activation by ATP, which has a low level in extracellular space and plasma (By similarity). Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Plays a role in the modulation of circadian clock function. NAMPT-dependent oscillatory production of NAD regulates oscillation of clock target gene expression by releasing the core clock component: CLOCK-ARNTL/BMAL1 heterodimer from NAD-dependent SIRT1-mediated suppression.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG207867 | Nampt (tGFP-tagged) - Mouse pre-B-cell colony-enhancing factor 1 (Pbef1) |
CNY 5,256.00 |
|
| MR207867 | Nampt (Myc-DDK-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 3,656.00 |
|
| MR207867L3 | Lenti ORF clone of Nampt (Myc-DDK-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 5,230.00 |
|
| MR207867L4 | Lenti ORF clone of Nampt (mGFP-tagged) - Mouse nicotinamide phosphoribosyltransferase (Nampt) |
CNY 5,230.00 |
