Tnfrsf1a (BC004599) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	p55, CD120a, TNF-R1, TNF-R55, TNFRp55, FPF, TNF-R, TNFAR, p55-R, TNFR60, TNF-R-I
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC004599 GAACTCTGGCTCATGATCGGGCCTACTGGGTGCGAGGTCCTGGAGGACCGTACCCTGATCTCTATCTGCC TCTGACTTTCAGCTTCTCGAACTCGAGGCCCAGGCTGCCATCGCCCGGGCCACCTGGTCCGATCATCTTA CTTCATTCACGAGCGTTGTCAATTGCTGCCCTGTCCCCAGCCCCAATGGGGGAGTGAGAGGCCACTGCCG GCCGGACATGGGTCTCCCCACCGTGCCTGGCCTGCTGCTGTCACTGGTGCTCCTGGCTCTGCTGATGGGG ATACATCCATCAGGGGTCACTGGACTAGTCCCTTCTCTTGGTGACCGGGAGAAGAGGGATAGCTTGTGTC CCCAAGGAAAGTATGTCCATTCTAAGAACAATTCCATCTGCTGCACCAAGTGCCACAAAGGAACCTACTT GGTGAGTGACTGTCCGAGCCCAGGGCGGGATACAGTCTGCAGGGAGTGTGAAAAGGGCACCTTTACGGCT TCCCAGAATTACCTCAGGCAGTGTCTCAGTTGCAAGACATGTCGGAAAGAAATGTCCCAGGTGGAGATCT CTCCTTGCCAAGCTGACAAGGACACGGTGTGTGGCTGTAAGGAGAACCAGTTCCAACGCTACCTGAGTGA GACACACTTCCAGTGCGTGGACTGCAGCCCCTGCTTCAACGGCACCGTGACAATCCCCTGTAAGGAGACT CAGAACACCGTGTGTAACTGCCATGCAGGGTTCTTTCTGAGAGAAAGTGAGTGCGTCCCTTGCAGCCACT GCAAGAAAAATGAGGAGTGTATGAAGTTGTGCCTACCTCCTCCGCTTGCAAATGTCACAAACCCCCAGGA CTCAGGTACTGCGGTGCTGTTGCCCCTGGTTATCTTGCTAGGTCTTTGCCTTCTATCCTTTATCTTCATC AGTTTAATGTGCCGATATCCCCGGTGGAGGCCCGAAGTCTACTCCATCATTTGTAGGGATCCCGTGCCTG TCAAAGAGGAGAAGGCTGGAAAGCCCCTAACTCCAGCCCCCTCCCCAGCCTTCAGCCCCACCTCCGGCTT CAACCCCACTCTGGGCTTCAGCACCCCAGGCTTTAGTTCTCCTGTCTCCAGTACCCCCATCAGCCCCATC TTCGGTCCTAGTAACTGGCACTTCATGCCACCTGTCAGTGAGGTAGTCCCAACCCAGGGAGCTGACCCTC TGCTCTACGAATCACTCTGCTCCGTGCCAGCCCCCACCTCTGTTCAGAAATGGGAAGACTCCGCCCACCC GCAACGTCCTGACAATGCAGACCTTGCGATTCTGTATGCTGTGGTGGATGGCGTGCCTCCAGCGCGCTGG AAGGAGTTCATGCGTTTCATGGGGCTGAGCGAGCACGAGATCGAGAGGCTGGAGATGCAGAACGGGCGCT GCCTGCGCGAGGCTCAGTACAGCATGCTGGAAGCCTGGCGGCGCCGCACGCCGCGCCACGAGGACACGCT GGAAGTAGTGGGCCTCGTGCTTTCCAAGATGAACCTGGCTGGGTGCCTGGAGAATATCCTCGAGGCTCTG AGAAATCCCGCCCCCTCGTCCACGACCCGCCTCCCGCGATAAAGCCACACCCACAACCTTAGGAAGAGGG ACTTGAACTTCAAGGACCATCCTGCTAGATGCCCTACTCCCTGTGGGTGAAAAGTGGGCAAAGGTCTCTA AGGGGGAAGGCTCGAGCTGGTAGCCACTTCCTTGGTGCTACCGACTTGGTGTACATAGCTTTTCTCAGCC GCCGAGGACTGCCTGAGCCAGCCACTTGTGAGTGGCAGGGAGATGTACCATCAGCTCCTGGCCAGCTGAG GGTGCCAAAGACAGGATTGTAGAGGAAAGGCACAATGTATCTGGTGCCCACTTGGGATGCACAGGGCCCA AGCCAAGCTTCTCAGGGCCTCCTCAGTGGGTTTCTGGGCCTTTTTCACTTTTGATAAGCAATCTTTGTAT CAATTATATCACACTAATGGATGAACTGTGTAAGGTAAGGACAAGCATAGAAAGGCGGGGTCTCCAGCTG GAGCCCTCGACTCTTGTAAATACACTAAACGTCTAAAAATGAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC004599
Insert Size	1365 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC004599, AAH04599
RefSeq Size	2086 bp
RefSeq ORF	1365 bp
Locus ID	21937
Gene Summary	This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mice lacking a functional copy of this gene exhibit impaired immune function. [provided by RefSeq, Sep 2016]

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