Mcl1 (BC003839) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AW556805; Mcl-1
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003839 CGCCTGCCTCCGGTCTGGAGTCGCGGCCTTCCCCGCTCCTTCCCCTCAGCCTGCGGCGTCCGACCATGTT TGGCCTGCGGAGAAACGCGGTCATCGGCTTGAACCTGTACTGCGGCGGCGCCAGCCTCGGCGCGGGCGGC GGTTCTCCGGCAGGGGCGCGCCTGGTGGCCGAGGAGGCCAAGGCGCGGCGCGAGGGGGGAGGGGAGGCCG CCCTGCTGCCCGGCGCGCGGGTGGTCGCCCGGCCGCCGCCCGTGGGCGCCGAGGACCCCGACGTCACCGC GTCGGCCGAAAGGCGGCTGCATAAGTCGCCCGGCCTCCTCGCCGTGCCGCCCGAGGAGATGGCCGCGTCG GCCGCCGCCGCCATCGTGTCTCCGGAGGAGGAACTGGACGGCTGCGAGCCGGAGGCCATCGGCAAGCGCC CGGCCGTGCTGCCCCTCCTGGAGCGCGTGAGCGAGGCGGCCAAGAGCTCCGGGGCCGACGGCTCTCTGCC CTCCACGCCGCCGCCGCCCGAGGAGGAAGAGGACGACCTATACCGCCAGTCGCTGGAGATCATCTCGCGC TACTTGCGGGAGCAGGCGACCGGCTCCAAGGACTCGAAGCCTCTGGGCGAGGCGGGCGCGGCGGGCCGGA GAGCGCTGGAGACCCTGCGGCGCGTGGGCGACGGCGTGCAGCGCAACCACGAGACGGCCTTCCAGGGCAT GCTCCGGAAACTGGACATTAAAAACGAAGGCGATGTTAAATCTTTTTCTCGAGTAATGGTCCATGTTTTC AAAGATGGCGTAACAAACTGGGGCAGGATTGTGACTCTTATTTCTTTCGGTGCCTTTGTGGCCAAACACT TAAAGAGCGTAAACCAAGAAAGCTTCATCGAACCATTAGCAGAAACTATCACAGATGTTCTTGTAAGGAC GAAACGGGACTGGCTTGTCAAACAAAGAGGCTGGGATGGGTTTGTGGAGTTCTTCCACGTACAGGACCTA GAAGGCGGCATCAGAAATGTGCTGCTGGCTTTTGCGGGTGTTGCTGGAGTAGGGGCTGGTCTGGCATATC TAATAAGATAGCCTTGTGAGTGCAATAGGGGACTCTTAAAGCTCCAGCCACCAAACTACATGCATCTGTG AAAACATGTGTATTTATGAAGGTGGACTTGAAGCTGCCCAGGATTTTAACAGTCCAGTTCTACTGTAGCA ACATAGCAAAAAGAAAGTGGCTACAGGATTGTGGCTAACAAGAATAAATACATGGGAAAAGTGAAAAAAA AAAAAAAAAAAAAAAA
Restriction Sites	RsrII-NotI
ACCN	BC003839
Insert Size	996 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003839, AAH03839
RefSeq Size	1276 bp
RefSeq ORF	996 bp
Locus ID	17210
Gene Summary	Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 2 has antiapoptotic activity.[UniProtKB/Swiss-Prot Function]

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