United States
Germany
Japan
United Kingdom
China
Nov (NM_010930) Mouse Untagged Clone
CAT＃: MC200106
Nov (untagged) - Mouse nephroblastoma overexpressed gene (Nov), (10ug)
CNY 3,656.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | C130088N23Rik; CCN3 |
| Vector | PCMV6-Kan/Neo |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC003774 sequence for NM_010930
GTGAAGGGGCAGTCCCATCTCCAGCCTGTAACAGCCCTAGCAGGCAGAACATGAGCCTCTTCCTGCGAAA GCGATGCCTCTGCCTAGGCTTCCTGCTCTTCCATCTCTTAAGTCAAGTCTCTGCATCTCTGCGCTGCCCT TCTCGGTGCCCACCCAAGTGCCCCAGTATATCACCGACCTGCGCCCCCGGGGTGCGCTCGGTGCTGGACG GCTGCTCCTGCTGTCCGGTGTGCGCCCGCCAGCGCGGGGAGAGTTGTTCTGAGATGAGACCCTGTGACCA GAGCAGTGGTCTCTACTGTGACCGCAGCGCAGACCCCAACAACCAGACTGGCATTTGCATGGTTCCAGAG GGAGACAACTGTGTGTTCGATGGGGTCATTTACCGCAACGGAGAGAAGTTTGAGCCGAACTGTCAGTACT TCTGCACCTGCAGAGATGGGCAGATTGGCTGTCTGCCCCGCTGCCAGCTAGACGTGCTACTGCCGGGTCC TGACTGCCCAGCTCCGAGAAAAGTCGCAGTGCCAGGGGAGTGCTGCGAAAAGTGGACCTGTGGCTCAGAC GAGCAGGGGACGCAGGGGACGCTGGGAGGCTTGGCCCTTCCAGCCTATAGACCGGAAGCCACCGTAGGAG TTGAAGTCTCTGACTCCAGCATTAACTGCATTGAGCAGACGACGGAGTGGAGCGCATGTTCCAAGAGCTG TGGAATGGGCGTGTCCACCCGGGTCACCAACAGGAATCGCCAGTGTGAGATGGTAAAACAGACTCGTCTC TGCATCGTTCGGCCTTGTGAACAAGAGCCCGAGGAAGTAACAGACAAGAAAGGTAAAAAGTGTCTCCGCA CCAAGAAGTCCCTGAAAGCCATCCACCTACAGTTCGAGAATTGCACTAGCTTGTACACCTATAAGCCCCG GTTCTGTGGGGTCTGCAGTGATGGCCGCTGCTGTACCCCCCACAACACCAAAACCATCCAGGTGGAGTTT CAGTGTCTCCCGGGGGAAATCATCAAGAAACCAGTCATGGTCATTGGAACCTGTACCTGCTACTCCAACT GCCCTCAGAACAATGAGGCCTTCCTCCAAGATCTGGAGCTGAAGACAAGCAGAGGAGAAATTTAACAGGT GCTCAGGGAAGCATATTGGTTGAGGCAAATGGTTGCTGCGGCATGGCCCACCATCTGTATATAGGAAACA TAATGAAGTTGTCCCACATATGTTGCTGTGATCATATGAGGCCATTTATATTTGTCTTTTTTTTTTTAAT GAAAGATGTTCCGAATGTAAACTTAGAATCAAGGTAAGCTCAGGATATGGCTTAAAGGTGACTTCCTTTC CTGTGCTGTTTATTACAAGAGCAAATTTATATAAATTTAAGTCACATAAATGATCTAGTTAGTAGAATGT GTCACATCACATTCATTTTCTGTGCACTAGTTAAATCCCAATAAATGTTACAGAAATGATTGACACAGTG AAATCTAAGATCATGTTGAATATATATATATATAATACCAAGGTTGACTTGAGGGATCTCTACTGAGTTC CTTGTTGGGGTAGGTCAACTTGTGCTTCCAAGTGTAAGATCAAAGAAAACATACATCTCTTGAACATGAA GTTCAGAGACTGATTTTCTACTTAGTGCACCAGGAAATATGCTATAGTTACAGCAGTAGAGTGTCTCTTA ATTACGTGGGCTAGTGTCATGAACTTTCTCCATTATAGACAAACCGACTCCTTTCCAATAGAAAGAAGCT GAACCAAAAACTCCCAATGCAGAGATGACGGGGCCTCTCAGCTCATGGTTTGTACCCCAGAGGCTGCTGA GGCCCACTTTTTCAAAATTAAACAGGGACTCTTTCATAGTTAAAGTACACTGAAGACCAAGCCACCTAAT TTACAATCTCTGATGATTTTCAAATGTGCTTTTAGTGCAATTTTGAAAAGGAAGAGCAGAATCTACAAAA TTTTATGAAAGTTTAAAGTAGCTGAAGACCTGCTCAAAATACATCTTTTGGTCATAGTTATGAAAACACA AGTTATACATGTGTACCTATGTATGCTTAATAAACAAAGTTCACAAAATGTGCTAGAAAATACAATTTGT ACATCACCATACACATCCCACTTTTCTTTTTAAAGTATTTATGAAAATATAAATTATACATTTTGTAAAA TATTCTCTTTAATTTCCCTAGTTGTTACAAACAACTTTTAAAAAGTATGATGTTATCAGTGAGTGACTCT GGTATTAGTTTTGTGTTATCAGTTTAATGCTGTTTACTGAAAATAAAAATCCATGAATTAAAAAAAAAAA AAAA |
| Restriction Sites | RsrII-NotI |
| ACCN | NM_010930 |
| Insert Size | 1065 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC003774, AAH03774 |
| RefSeq Size | 2314 bp |
| RefSeq ORF | 1065 bp |
| Locus ID | 18133 |
| UniProt ID | Q64299 |
| Gene Summary | Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival. Acts by binding to integrins or membrane receptors such as NOTCH1. Essential regulator of hematopoietic stem and progenitor cell function. Inhibits myogenic differentiation through the activation of Notch-signaling pathway. Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling. Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival. Plays also a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts (By similarity). Involved in bone regeneration as a negative regulator (PubMed:23653360). Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification (By similarity). Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion (PubMed:23705021). Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway (By similarity). Contributes to the control and coordination of inflammatory processes in atherosclerosis (PubMed:24722330). Attenuates inflammatory pain through regulation of IL1B- and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG205398 | Nov (tGFP-tagged) - Mouse nephroblastoma overexpressed gene (Nov) |
CNY 3,140.00 |
|
| MR205398 | Nov (Myc-DDK-tagged) - Mouse nephroblastoma overexpressed gene (Nov) |
CNY 3,656.00 |
|
| MR205398L3 | Lenti ORF clone of Nov (Myc-DDK-tagged) - Mouse nephroblastoma overexpressed gene (Nov) |
CNY 4,750.00 |
|
| MR205398L4 | Lenti ORF clone of Nov (mGFP-tagged) - Mouse nephroblastoma overexpressed gene (Nov) |
CNY 4,750.00 |
