Dusp6 (BC003869) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MKP3, PYST1, MKP-3
Vector	PCMV6-Kan/Neo
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >BC003869 GGCGTCTGCTAGGCACCCCGCCTTCTCCTGCAGCTCGACCCCCATGATAGATACGCTCAGACCCGTGCCT TTCGCGTCGGAAATGGCGATCTGCAAGACGGTGTCGTGGCTCAACGAGCAGCTGGAGCTGGGCAACGAAC GGCTTCTGCTGATGGACTGCCGACCACAGGAGCTGTACGAGTCGTCGCACATCGAATCTGCCATTAATGT GGCCATCCCGGGCATCATGCTGCGGCGTCTGCAGAAGGGCAACCTGCCCGTGCGTGCGCTCTTCACGCGC TGCGAGGACCGGGACCGCTTTACCAGGCGCTGCGGCACCGACACCGTGGTGCTGTACGACGAGAATAGCA GCGACTGGAATGAGAACACTGGTGGAGAGTCGGTCCTCGGGCTGCTGCTCAAGAAACTCAAAGACGAGGG CTGCCGGGCGTTCTACCTGGAAGGTGGCTTCAGTAAGTTCCAGGCCGAGTTCGCCCTGCACTGCGAGACC AATCTAGACGGCTCGTGCAGCAGCAGTTCCCCGCCTTTGCCAGTGCTGGGGCTCGGGGGCCTGCGGATCA GCTCCGACTCTTCCTCGGACATTGAGTCTGACCTTGACCGAGACCCCAATAGTGCAACGGACTCTGATGG CAGCCCGCTGTCCAACAGCCAGCCTTCCTTCCCGGTGGAGATTTTGCCCTTCCTTTACCTGGGCTGTGCC AAGGACTCGACCAACTTGGACGTGTTGGAAGAGTTTGGCATCAAGTACATCTTGAATGTCACCCCCAATT TGCCCAATCTGTTTGAGAATGCGGGCGAGTTCAAATACAAGCAAATTCCTATCTCGGATCACTGGAGCCA AAACCTGTCCCAGTTTTTCCCTGAGGCCATTTCTTTCATAGATGAAGCCCGAGGCAAAAACTGTGGTGTC CTGGTGCATTGCTTGGCAGGTATCAGCCGCTCTGTCACCGTGACAGTGGCGTACCTCATGCAGAAGCTCA ACCTGTCCATGAACGATGCTTACGACATTGTTAAGATGAAGAAGTCCAACATCTCCCCCAACTTCAACTT CATGGGCCAGCTGCTTGACTTCGAAAGGACCCTGGGACTCAGCAGCCCTTGTGACAACCGCGTCCCCACT CCGCAGCTGTACTTCACCACGCCCTCCAACCAGAACGTCTACCAGGTGGACTCCCTGCAGTCTACGTGAA AGGCACCCACCTCTCCTAGCCGGGAGTTGTCCCCATTCCTTCAGTTCCTCTTGAGCAGCATCGACCAGGC TGCTTTCTTTCTGTGTGTGGCCCCGGGTGTCAAAAGTGTCACCAGCTGTCTGTGTTAGACAAGGTTGCCA AGTGCAAAATTGGTTATTACGGAGGGAGAGATTTGCTCCATTCATTGTTTTTTTGGAAGGACAGGACATG CTGTCTCTAGATCCAGCAATAGGTTTGCTTCTGTACCCCAGCCTACCCAAGCAGGGACTGGACATCCATC CAGATAGAGGGTAGCATAGGAATAGGGACAGGAGCATCTGTTCTTTAAGGCCTTGTATGGCTGTTTCCTG TTGCATCTGGAACTAACTATATATATTGTCTTCAGTGAAGACTGATTCAACTTTGGGTATAGTGGAGCCA AAGAGATTTTTAGCTCTGTATTTGCGGTATCGGTTTAGAAGACAAAAAAAATTAAAACCTGATACTTTTA TCTGATTATTGTAAATATTTGATCTTCAATCACTTGACAGTGTTTGTTTGGCTTGTATTTGTTTTTTATC TTTGGGCTTAAAAGAGATCCAAAGAGAGAAAGAGCAGTATGCCACTTCAAAAGTATATGGAAAAAAATGT TCTTTTTAATCCAAAGGGTATATTTGCAGCATGCTTGACCTTGATGTACCAATTCTGACGGCATTTTCGT GGATATTATCACTAAGACTTTGTTATGATGAGGTCTTCAGTCTCTTTCATATATCTTCCTTGTAACTTTT TTTTTCCTCTTAATGTAGTTTTGACTCTGCCTTACCTTTGTAAATATTTGGCTTACAGTGTCTCAAGGGG TATTTTGGAAAAACACCAAAATTGTGGGTTCACTTTTTTTTTTTTTAAATAACTTCAGCTGTGCTAAACA GCATATTACCTCTGTACAAAATTCTTCAGGGAGTGTCACCTCAAATGCAATACTTTGGTTTGGTTTCTTT CCTTTTAAAAAAAAATACGAAACTGGAAGTGTGTGTATGTGTGCGAGTATGAGCGCCGATTTGGTGGATG CAACAGGTTGAGAGGAAGGGAGAATTAACTTGCTCCATGATGTTCGTGGTGTAAAGTTTTGAGCTGGAAT TTATTATAAGAATGTAAAACCTTAAATTATTAATAAATAACTATTTTGGCTATTGAAAAAAAAAAAAAAA AAA
Restriction Sites	RsrII-NotI
ACCN	BC003869
Insert Size	1146 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC003869, AAH03869
RefSeq Size	2383 bp
RefSeq ORF	1146 bp
Locus ID	67603
Gene Summary	Inactivates MAP kinases. Has a specificity for the ERK family (By similarity). Plays an important role in alleviating acute postoperative pain (PubMed:24155322, PubMed:28405172). Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (PubMed:24155322). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (PubMed:28405172).[UniProtKB/Swiss-Prot Function]

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