Rela (BC003818) Mouse Untagged Clone

CAT#: MC200064

Rela (untagged) - Mouse v-rel reticuloendotheliosis viral oncogene homolog A (avian) (cDNA clone MGC:6177 IMAGE:3489295), (10ug)



CNY 4,096.00


货期*
现货

规格
    • 10 ug

Cited in 1 publication.

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

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DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

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Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

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Anti-NF-kB p65 (Ser316) Antibody
    • 100 ul

CNY 5,325.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms p65
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC003818
CGACGACGGGCGCGCGCCGCGCATTTCCGCCTCTGGCGAATGGCTTTACTTTAGCGCGCCGTGGGCTCAG CTGCGACCCCGGCCCCGCCCCCGGGACCCTGACCATGGACGATCTGTTTCCCCTCATCTTTCCCTCAGAG CCAGCCCAGGCTTCTGGGCCTTATGTGGAGATCATCGAACAGCCGAAGCAACGGGGCATGCGATTCCGCT ATAAATGCGAGGGGCGCTCAGCGGGCAGTATTCCTGGCGAGAGAAGCACAGATACCACCAAGACACACCC CACCATCAAGATCAATGGCTACACAGGACCAGGAACAGTTCGAATCTCCCTGGTCACCAAGGATCCACCT CACCGGCCTCATCCACATGAACTTGTGGGGAAGGACTGCCGGGATGGCTACTATGAGGCTGACCTCTGCC CAGACCGCAGTATCCATAGCTTCCAGAACCTGGGGATCCAGTGTGTGAAGAAGCGAGACCTGGAGCAAGC CATTAGCCAGCGAATCCAGACCAACAATAACCCCTTTCACGTTCCTATAGAGGAGCAGCGCGGGGACTAT GACTTGAATGCAGTGCGCCTCTGCTTCCAGGTGACAGTGCGGGACCCAGCAGGCAGGCCCCTCCTCCTGA CCCCTGTCCTCTCACATCCGATTTTTGATAACCGGGCCCCCAACACTGCCGAGCTCAAGATCTGCCGAGT AAACCGGAACTCTGGGAGCTGCCTCGGTGGGGATGAGATCTTCTTGCTGTGCGACAAGGTGCAGAAAGAA GACATTGAGGTGTATTTCACGGGACCAGGCTGGGAGGCACGAGGCTCCTTTTCTCAAGCTGATGTGCATC GGCAAGTGGCCATTGTGTTCCGGACTCCTCCGTACGCCGACCCCAGCCTCCAGGCTCCTGTTCGAGTCTC CATGCAGCTACGGCGGCCTTCTGATCGCGAGCTCAGTGAGCCCATGGAGTTCCAGTACTTGCCAGACACA GATGATCGCCACCGGATTGAAGAGAAGCGCAAAAGGACCTATGAGACCTTCAAGAGTATCATGAAGAAGA GTCCTTTCAATGGACCAACTGAACCCCGGCCTCCAACCCGGCGTATTGCTGTGCCTACCCGAAACTCAAC TTCTGTCCCCAAGCCAGCCCCGCAGCCCTACACCTTCCCAGCATCCCTCAGCACCATCAACTTTGATGAG TTTTCCCCCATGCTGTTACCATCAGGGCAGATCTCAAACCAGGCCCTGGCCTTAGCACCGTCCTCTGCCC CAGTCCTTGCCCAGACCATGGTCCCTTCCTCAGCCATGGTACCTCTGGCTCAGCCCCCAGCTCCTGCCCC AGTTCTAACCCCGGGTCCTCCCCAGTCCCTGTCTGCACCTGTTCCAAAGAGCACCCAGGCTGGGGAAGGC ACGCTGTCGGAAGCCCTGCTGCACCTGCAGTTTGATGCTGATGAAGACTTGGGGGCCTTGCTTGGCAACA GCACAGACCCAGGAGTGTTCACAGACCTGGCATCTGTGGACAACTCAGAGTTTCAGCAGCTCCTGAACCA GGGTGTGTCCATGTCTCACTCCACAGCTGAGCCCATGCTGATGGAGTACCCTGAAGCTATAACTCGCCTG GTGACAGGGTCCCAGAGGCCCCCTGACCCAGCTCCCACACCCCTGGGGACCTCGGGGCTTCCCAATGGTC TCTCCGGAGATGAAGACTTCTCCTCCATTGCGGACATGGACTTCTCTGCTCTTTTGAGTCAGATCAGCTC CTAAGGTGCTGACAGCGACCCTGCTCAGAGCACCAGGTTTCAGGGCACTGAAGCCTTCCCGAAGTGCGTA CACATTCTGGGGAGTGTGCTCCAGCTGCCCCCGACTTGTTTGGGTGATCTCTCTGGGGCGGCACGTTTTA CTCTTTATCTCGCTTTCGGAGGTGCTTTCGCAGGAGCATTAACCTCCTGGAGACGGAGCTGGGAGGACTC GGTGCATCCCTGTGTTGATAGCTCCTGCTTCGGTAGGGAACTCTGAGATCCTGCTTCCATCTCCAGCTTC TAGCACTCTCCTAGAGAGGGACAGACTGGAGCCATGGCCTTAGGCCATATAGCCTTACTATCAAGTGTCT TCCTCCACGCGGATTCCTGTACACCTTGATCCAAAGCAGTGCTCCCAAGAGCAGCTCCTACGTGGTGCTG CCCGACACCAGCACATGAGGGGCCGCTCTTCTGTCCTGTGGAGCTCCTGCCCTGCCAGCTCTCCATGCTG AGCTGTGGCCAAGGGGAACAGGTGGGATGTTGCTGGCCGCCTTCAGAATCAGGGGGAGTTTAGTCTGAGA CATCCCTGCTCCCCCTTTTTTCAAGTGCCTTAATAGCAGGGCAAACTGTAGAGTCAGGAGGGCAGGCTAG ATGCTCAGCCACAAGACAGCCTTTACTGAAAAAGCTATTGGACTCTTGCTCTTTCTAGCTCTGAACTAAT AAATGTCTTATCACGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites RsrII-NotI     
ACCN BC003818
Insert Size 1650 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC003818, AAH03818
RefSeq Size 2497 bp
RefSeq ORF 1650 bp
Locus ID 19697
Gene Summary NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric RELA-NFKB1 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. The NF-kappa-B heterodimeric RELA-NFKB1 and RELA-REL complexes, for instance, function as transcriptional activators. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The inhibitory effect of I-kappa-B on NF-kappa-B through retention in the cytoplasm is exerted primarily through the interaction with RELA. RELA shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Beside its activity as a direct transcriptional activator, it is also able to modulate promoters accessibility to transcription factors and thereby indirectly regulate gene expression (PubMed:29813070). Associates with chromatin at the NF-kappa-B promoter region via association with DDX1. Essential for cytokine gene expression in T-cells (By similarity). The NF-kappa-B homodimeric RELA-RELA complex appears to be involved in invasin-mediated activation of IL-8 expression (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

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